##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1045925
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300115209025503	32.0	32.0	32.0	32.0	32.0
2	31.464446303511245	32.0	32.0	32.0	32.0	32.0
3	31.583702464325835	32.0	32.0	32.0	32.0	32.0
4	31.657799555417455	32.0	32.0	32.0	32.0	32.0
5	31.631683916150777	32.0	32.0	32.0	32.0	32.0
6	35.279648158328754	36.0	36.0	36.0	36.0	36.0
7	35.27682960059278	36.0	36.0	36.0	36.0	36.0
8	35.232422974878695	36.0	36.0	36.0	36.0	36.0
9	35.30046705069675	36.0	36.0	36.0	36.0	36.0
10	35.19107488586658	36.0	36.0	36.0	36.0	36.0
11	35.31135406458398	36.0	36.0	36.0	36.0	36.0
12	35.23124220187872	36.0	36.0	36.0	36.0	36.0
13	35.26877548581399	36.0	36.0	36.0	36.0	36.0
14	35.23180055931353	36.0	36.0	36.0	36.0	36.0
15	35.204877022731075	36.0	36.0	36.0	36.0	36.0
16	35.2159542988264	36.0	36.0	36.0	36.0	36.0
17	35.2000936969668	36.0	36.0	36.0	36.0	36.0
18	35.19703420417334	36.0	36.0	36.0	36.0	36.0
19	35.18537849272175	36.0	36.0	36.0	36.0	36.0
20	35.18675717666181	36.0	36.0	36.0	36.0	36.0
21	35.18399694050721	36.0	36.0	36.0	36.0	36.0
22	35.164989841527834	36.0	36.0	36.0	36.0	36.0
23	35.135297463967305	36.0	36.0	36.0	36.0	36.0
24	35.112413413963715	36.0	36.0	36.0	36.0	36.0
25	35.099814996295144	36.0	36.0	36.0	36.0	36.0
26	35.05859884790974	36.0	36.0	36.0	36.0	36.0
27	35.05072639051557	36.0	36.0	36.0	36.0	36.0
28	35.03144871764228	36.0	36.0	36.0	36.0	36.0
29	35.00468293615699	36.0	36.0	36.0	36.0	36.0
30	34.988693261945166	36.0	36.0	36.0	36.0	36.0
31	34.996265506608985	36.0	36.0	36.0	36.0	36.0
32	34.97175323278438	36.0	36.0	36.0	36.0	36.0
33	34.95304921480986	36.0	36.0	36.0	36.0	36.0
34	34.94075674642063	36.0	36.0	36.0	36.0	36.0
35	34.927160169228195	36.0	36.0	36.0	36.0	36.0
36	34.90854697994598	36.0	36.0	36.0	32.0	36.0
37	34.88532925400961	36.0	36.0	36.0	32.0	36.0
38	34.87504840213209	36.0	36.0	36.0	32.0	36.0
39	34.86736429476301	36.0	36.0	36.0	32.0	36.0
40	34.84855415063222	36.0	36.0	36.0	32.0	36.0
41	34.83070105409087	36.0	36.0	36.0	32.0	36.0
42	34.786960824150874	36.0	36.0	36.0	32.0	36.0
43	34.793959413915914	36.0	36.0	36.0	32.0	36.0
44	34.74533451251285	36.0	36.0	36.0	32.0	36.0
45	34.735081387288766	36.0	36.0	36.0	32.0	36.0
46	34.7163515548438	36.0	36.0	36.0	32.0	36.0
47	34.69260893467505	36.0	36.0	36.0	32.0	36.0
48	34.66394817984081	36.0	36.0	36.0	32.0	36.0
49	34.65631283313813	36.0	36.0	36.0	32.0	36.0
50	34.23592226976122	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	11.0
21	37.0
22	88.0
23	213.0
24	538.0
25	1144.0
26	2478.0
27	4642.0
28	8253.0
29	12583.0
30	19307.0
31	28345.0
32	42829.0
33	71892.0
34	166716.0
35	686845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.14342987647426	17.6751988309239	12.0552365504604	26.126134742141442
2	16.4204463422633	19.538875912031887	36.49389652149054	27.54678122421427
3	18.815743430198427	22.697007998684416	28.18135577987651	30.305892791240645
4	12.932476037956834	15.524153261467122	34.481917919544905	37.06145278103114
5	15.241532614671224	36.067308841456125	32.54736238258001	16.143796161292638
6	35.97313382890743	33.9780577001219	15.998279035303678	14.050529435666995
7	31.564500322680882	29.74955183211033	19.447379114181228	19.23856873102756
8	28.86507158735091	32.70588235294118	18.492530535172218	19.936515524535697
9	27.213136697181923	14.286205989913233	18.3855438965509	40.11511341635394
10	17.213184501756817	26.115830484977415	29.972129932834573	26.6988550804312
11	37.66570260773956	21.001410234959486	21.431651409039844	19.901235748261108
12	24.875134330983894	23.314791514488626	27.69130453073072	24.11876962379676
13	29.662356287496717	18.94084183856395	24.881516361115757	26.515285512823578
14	23.946076439515263	19.153667806009032	24.449649831488873	32.45060592298683
15	25.566651528551283	26.59817864569639	21.635203288954752	26.199966536797568
16	26.336149365723443	25.505394314648594	22.878797150057938	25.279659169570024
17	24.64383201472381	25.830628391137033	24.38855558476946	25.136984009369694
18	25.48705784269014	24.538230825787366	25.297273317443064	24.67743801407943
19	26.019456461983413	24.68714295958123	24.761144441523054	24.532256136912302
20	26.240459383721365	23.994213723189947	24.316799611443674	25.448527281645017
21	27.078327795970075	23.7743624064823	24.20881038315367	24.93849941439396
22	26.506967516791356	23.570906135717188	24.596601094724765	25.32552525276669
23	24.969859215526927	23.75093816478237	25.119009489208118	26.16019313048259
24	25.25164448523788	24.37289658864923	24.88278262199786	25.492676304115037
25	25.431866942531194	23.877069462025922	24.933169140396434	25.757894455046447
26	25.200350314269375	24.840283846343596	25.151971760692522	24.807394078694507
27	25.77608782324444	24.26086532513512	24.53497833006189	25.428068521558554
28	25.33471013141729	24.05725185365784	25.181254511643026	25.426783503281847
29	25.138491309992983	24.42762980512738	25.13485812409051	25.299020760789126
30	25.14473056451374	24.42946757318275	25.21509997523685	25.210701887066655
31	25.654281067654377	24.222239007035917	24.512223221874734	25.61125670343497
32	25.649910030843838	24.174454113818154	24.2997990274535	25.875836827884513
33	25.150656693956808	24.052868237658483	24.812390228325086	25.984084840059623
34	25.81679599508947	24.171157148375205	24.88957048175953	25.122476374775793
35	26.113681408669663	23.878143993391483	25.159117831809795	24.849056766129056
36	24.95673673562656	24.57649805626041	24.685875237350395	25.780889970762637
37	26.13545207260698	24.271229222539333	24.567527643524027	25.02579106132966
38	25.251141334225686	24.01252479862323	25.008867748643542	25.727466118507543
39	25.848251676042818	24.073443756477573	24.369547841318038	25.708756726161564
40	25.9928560638365	24.20916664913827	24.78932463415054	25.00865265287469
41	25.087053046426377	24.67803280193209	25.04297676482005	25.19193738682148
42	26.23206156794015	24.571528556635677	24.561202215573843	24.635207659850327
43	25.740879733431495	23.870119563814377	24.772035166390186	25.61696553636394
44	25.25784383560334	24.424769453620616	24.602322435066952	25.71506427570909
45	25.433477929555476	24.601276793883933	24.508342567795612	25.45690270876498
46	25.382899528731688	24.401162251494167	24.674419471823818	25.54151874795033
47	25.524013855783966	24.273914660532338	24.925782829803893	25.276288653879803
48	26.14564997690964	24.920570116771252	23.894268140752718	25.03951176556639
49	25.42401727143129	24.95417773779053	24.167765412964474	25.45403957781371
50	25.62637022220905	24.975451928326322	24.124133407654792	25.274044441809835
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	199.0
1	129.0
2	59.0
3	141.0
4	223.0
5	226.5
6	230.0
7	179.0
8	128.0
9	175.5
10	223.0
11	380.0
12	537.0
13	994.5
14	1452.0
15	1873.5
16	2295.0
17	2155.5
18	2016.0
19	1886.5
20	1757.0
21	2010.5
22	2264.0
23	2679.5
24	3095.0
25	3969.5
26	4844.0
27	6126.0
28	7408.0
29	8606.5
30	9805.0
31	11289.5
32	12774.0
33	14621.5
34	16469.0
35	19461.0
36	22453.0
37	27556.5
38	32660.0
39	36390.5
40	40121.0
41	44692.0
42	49263.0
43	52055.5
44	54848.0
45	60281.5
46	65715.0
47	70811.5
48	75908.0
49	79844.5
50	83781.0
51	82294.5
52	80808.0
53	81215.0
54	81622.0
55	82589.0
56	83556.0
57	81169.0
58	78782.0
59	72334.5
60	65887.0
61	58019.0
62	50151.0
63	43232.0
64	36313.0
65	30633.0
66	24953.0
67	21450.0
68	17947.0
69	15693.5
70	13440.0
71	10493.5
72	7547.0
73	6772.5
74	5998.0
75	4384.5
76	2771.0
77	2492.0
78	2213.0
79	1865.0
80	1517.0
81	1160.0
82	803.0
83	704.0
84	605.0
85	445.0
86	285.0
87	194.5
88	104.0
89	69.5
90	35.0
91	26.0
92	17.0
93	14.0
94	11.0
95	11.5
96	12.0
97	13.0
98	14.0
99	10.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029925663886033894
2	0.0043980208905992305
3	2.8682744938690635E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	9.560914979563545E-5
13	0.0
14	0.0
15	0.0
16	8.60482348160719E-4
17	0.0
18	1.912182995912709E-4
19	0.0
20	1.912182995912709E-4
21	0.0
22	0.0
23	0.0
24	4.780457489781772E-4
25	9.560914979563545E-5
26	6.692640485694481E-4
27	5.736548987738127E-4
28	0.0019121829959127088
29	0.0010517006477519899
30	0.0015297463967301672
31	5.736548987738127E-4
32	0.0010517006477519899
33	3.824365991825418E-4
34	8.60482348160719E-4
35	1.912182995912709E-4
36	2.8682744938690635E-4
37	0.0019121829959127088
38	0.0
39	8.60482348160719E-4
40	2.8682744938690635E-4
41	0.0010517006477519899
42	0.005354112388555585
43	0.0038243659918254175
44	0.0038243659918254175
45	0.0022946195950952508
46	0.0022946195950952508
47	7.648731983650836E-4
48	0.0032507110930516054
49	0.003441929392642876
50	0.001720964696321438
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1045925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.48020103712975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.48369566542077	46.482225016878445
2	12.475263292252814	14.591118106441394
3	3.5137816767702676	6.164599765743243
4	1.4927096871233598	3.4917585037218055
5	0.7902789519513213	2.3107835992762733
6	0.4979857902253755	1.7473385475608327
7	0.31168514805296954	1.2759187082897645
8	0.23484711058214214	1.098712499186617
9	0.17096571938602828	0.8998298676137202
>10	0.8896951859438984	9.337594852323521
>50	0.0745768899839888	3.0775418366105383
>100	0.058260889485674434	6.84068421816436
>500	0.0054310990290748935	2.1448418606299047
>1k	8.228937922840749E-4	0.5370526175595634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1232	0.11779047254822286	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1122	0.10727346607070298	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1093	0.10450080072662955	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1080	0.10325788177928628	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1053	0.10067643473480412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.912182995912709E-4
2	0.0	0.0	0.0	0.0	1.912182995912709E-4
3	0.0	0.0	0.0	0.0	1.912182995912709E-4
4	0.0	0.0	0.0	0.0	1.912182995912709E-4
5	0.0	0.0	0.0	0.0	1.912182995912709E-4
6	0.0	0.0	0.0	0.0	2.8682744938690635E-4
7	0.0	0.0	0.0	0.0	2.8682744938690635E-4
8	0.0	0.0	0.0	0.0	2.8682744938690635E-4
9	0.0	0.0	0.0	0.0	2.8682744938690635E-4
10	0.0	0.0	0.0	0.0	2.8682744938690635E-4
11	0.0	0.0	0.0	0.0	2.8682744938690635E-4
12	0.0	0.0	0.0	9.560914979563545E-5	0.0010517006477519899
13	0.0	0.0	0.0	9.560914979563545E-5	0.0012429189473432607
14	0.0	0.0	0.0	9.560914979563545E-5	0.0012429189473432607
15	0.0	9.560914979563545E-5	0.0	9.560914979563545E-5	0.0018165738461170735
16	0.0	9.560914979563545E-5	0.0	9.560914979563545E-5	0.0021034012955039798
17	0.0	9.560914979563545E-5	0.0	9.560914979563545E-5	0.0021034012955039798
18	0.0	9.560914979563545E-5	0.0	9.560914979563545E-5	0.0022946195950952508
19	0.0	9.560914979563545E-5	0.0	9.560914979563545E-5	0.0022946195950952508
20	0.0	9.560914979563545E-5	0.0	1.912182995912709E-4	0.0024858378946865213
21	0.0	9.560914979563545E-5	0.0	1.912182995912709E-4	0.0026770561942777923
22	0.0	9.560914979563545E-5	0.0	2.8682744938690635E-4	0.002772665344073428
23	0.0	9.560914979563545E-5	0.0	6.692640485694481E-4	0.002772665344073428
24	0.0	9.560914979563545E-5	0.0	7.648731983650836E-4	0.002772665344073428
25	0.0	9.560914979563545E-5	0.0	9.560914979563544E-4	0.002772665344073428
26	0.0	9.560914979563545E-5	0.0	0.0012429189473432607	0.002772665344073428
27	0.0	9.560914979563545E-5	0.0	0.0013385280971388962	0.002772665344073428
28	0.0	9.560914979563545E-5	0.0	0.0019121829959127088	0.002772665344073428
29	0.0	9.560914979563545E-5	0.0	0.0030594927934603344	0.002772665344073428
30	0.0	9.560914979563545E-5	0.0	0.00525850323875995	0.002772665344073428
31	0.0	9.560914979563545E-5	0.0	0.011664316275067524	0.002772665344073428
32	0.0	9.560914979563545E-5	0.0	0.016635992064440566	0.0028682744938690634
33	0.0	9.560914979563545E-5	0.0	0.02285058680115687	0.0028682744938690634
34	0.0	9.560914979563545E-5	0.0	0.028873963238281904	0.002963883643664699
35	0.0	9.560914979563545E-5	0.0	0.03537538542438511	0.002963883643664699
36	0.0	9.560914979563545E-5	0.0	0.04608361020149628	0.002963883643664699
37	0.0	1.912182995912709E-4	0.0	0.05985132777206779	0.002963883643664699
38	0.0	1.912182995912709E-4	0.0	0.07964242177976433	0.002963883643664699
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1345	0.0	27.156872	1
GGTATCA	620	0.0	23.778095	1
CGGATCG	75	2.0625976E-6	23.468273	26
ATACGAA	220	0.0	21.001436	40
CGAATGC	235	0.0	19.660921	43
TACGAAT	255	0.0	18.118887	41
GTATTAA	135	5.512993E-8	17.928867	1
TCAACGC	2020	0.0	17.642242	4
CGCAATA	280	0.0	17.285246	36
TAATACC	140	8.3760824E-8	17.28442	4
CAACGCA	2075	0.0	17.280632	5
AACGCAG	2075	0.0	17.280632	6
ATCAACG	2065	0.0	17.257788	3
GTCCTAA	90	2.2122369E-4	17.113918	1
TCGATTT	90	2.2138536E-4	17.112284	30
TCTAGCG	270	0.0	17.112282	28
TATCAAC	2155	0.0	16.84409	2
GTTATAG	170	2.4610927E-9	16.82629	1
CAATACG	275	0.0	16.801151	38
CTAGCGG	250	0.0	16.721144	29
>>END_MODULE