FastQCFastQC Report
Thu 2 Feb 2017
SRR4062209_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062209_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12392
Sequences flagged as poor quality0
Sequence length25
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT810.6536475145255003No Hit
ATCCTATTCCCATCCTCAAAACGCC760.6132989025177534No Hit
TATCAACGCAGAGTACTTTTTTTTT630.5083925112976113No Hit
CTCCGAGGTCACCCCAACCGAAATT550.4438347320852163No Hit
GGTATCAACGCAGAGTACTTTTTTT540.43576500968366694No Hit
ATCCTAGCCCTAGCCCTACACAAAT440.35506778566817304No Hit
CCATTGGGATGTCCTGATCCAACAT360.2905100064557779No Hit
TGCTTACACCACATGAAACACTGTC340.27437056165267915No Hit
AAGAGGGACAGCTCTTCTGGAACGG300.24209167204648158No Hit
ATCCTGACCGTGCAAAGGTAGCATA300.24209167204648158No Hit
ACGCAGAGTACTTTTTTTTTTTTTT280.22595222724338285No Hit
CATTTACACCTACTACCCAACTATC250.20174306003873468No Hit
CTTTTAGGCCTACAATGGTTAAAAG220.17753389283408652No Hit
ATTAATAAGTGTCCTGCAGTAATGT210.16946417043253711No Hit
GTCCAACTGTCTCTTATCTTTAATC210.16946417043253711No Hit
GTATTAGAGGCACTGCCTGCCCAGT200.16139444803098774No Hit
CATCTAGCCTATCAGTTTACTCCAT200.16139444803098774No Hit
CCCCTATACTATATCAATTTACCAG190.15332472562943836No Hit
TCCTAACACTCCTCGTCCCCATTCT190.15332472562943836No Hit
ATTCCATTCCATTCCATTCCATTCC180.14525500322788895No Hit
GATTAAACCTTGTACCTTTTGCATA170.13718528082633957No Hit
CCATAGGGTCTTCTCGTCTTATTAT170.13718528082633957No Hit
CACTAGCATTAAGTCTATGAGTTCC170.13718528082633957No Hit
GTGGTACTCCCGCTGTAAAAATTGG160.12911555842479017No Hit
ATCCTATTTAAGAGTTCATATCGAC160.12911555842479017No Hit
ATCCTACTCTCTACAAACACTTATT160.12911555842479017No Hit
CTTCTACACCATTGGGATGTCCTGA160.12911555842479017No Hit
CGTCTATGTGGCAAAATAGTGAGAA160.12911555842479017No Hit
ACCAATACCACACCCATTAATTAAT160.12911555842479017No Hit
CCAGAAGAGCTGTCCCTCTTTTGGC160.12911555842479017No Hit
CTCCTAGGCCTTTTACCACATACAT160.12911555842479017No Hit
GTATTGGAATTAGTGAAATTGGAGT160.12911555842479017No Hit
AGCATGAACGGCTAAACGAGGGTCC150.12104583602324079No Hit
GTTATATAATTTAAGCTCCATAGGG150.12104583602324079No Hit
GTTATAGATTAACCCAATTTTAAGT140.11297611362169142No Hit
ACCTATAACTTCTCTGTTAACCCAA140.11297611362169142No Hit
GCTATCACCAAGCTCGTTAGGCTTT130.10490639122014203No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG130.10490639122014203No Hit
TACTAGAGTAGCTCCTCCGATTAGG130.10490639122014203No Hit
TACTAACAGTGTTGCATCTATAAAG130.10490639122014203No Hit
TCTAGGAGCTATAGAACTAGTACCG130.10490639122014203No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT130.10490639122014203No Hit
GTTAGTATGAGTAACAAGAATTCCA130.10490639122014203No Hit
GCTGTAAGCCGGACTGCTAATGCCA130.10490639122014203No Hit
GAGTAGGATCCATTTATTAATAGAA130.10490639122014203No Hit
ACCTTGTACCTTTTGCATAATGAAC130.10490639122014203No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA350.002010796716.2857151
AACGCAG400.0048973114.256
ACGCAGA400.0048973114.257
TCAACGC400.0048973114.254
CAGAGTA400.0048973114.2510
AGAGTAC400.0048973114.2511
GCAGAGT400.0048973114.259
CGCAGAG400.0048973114.258
TATCAAC400.0048973114.252
CAACGCA400.0048973114.255
Produced by FastQC (version 0.11.5)