##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062209_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12392 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.971836668818593 32.0 14.0 32.0 14.0 32.0 2 23.879922530664945 32.0 14.0 32.0 14.0 32.0 3 24.366123305358297 32.0 14.0 32.0 14.0 32.0 4 23.95182375726275 21.0 14.0 32.0 14.0 32.0 5 24.55301807617818 27.0 14.0 32.0 14.0 32.0 6 27.825451904454486 32.0 21.0 36.0 14.0 36.0 7 27.61337959974177 32.0 21.0 36.0 14.0 36.0 8 27.2328114912847 32.0 21.0 36.0 14.0 36.0 9 27.26307295029051 32.0 14.0 36.0 14.0 36.0 10 26.154938670109747 32.0 14.0 36.0 14.0 36.0 11 28.16865719819238 32.0 21.0 36.0 14.0 36.0 12 26.594980632666235 32.0 14.0 36.0 14.0 36.0 13 27.320448676565526 32.0 21.0 36.0 14.0 36.0 14 26.57359586830213 32.0 14.0 36.0 14.0 36.0 15 26.747579083279536 32.0 14.0 36.0 14.0 36.0 16 26.864105874757907 32.0 14.0 36.0 14.0 36.0 17 26.500242091672046 32.0 14.0 36.0 14.0 36.0 18 26.40380890897353 32.0 14.0 36.0 14.0 36.0 19 26.50338928340865 32.0 14.0 36.0 14.0 36.0 20 26.501856036152358 32.0 14.0 36.0 14.0 36.0 21 26.558263395739186 32.0 14.0 36.0 14.0 36.0 22 26.18140735958683 32.0 14.0 36.0 14.0 36.0 23 26.170916720464817 32.0 14.0 36.0 14.0 36.0 24 26.03066494512589 32.0 14.0 36.0 14.0 36.0 25 25.165348612007747 27.0 14.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 0.0 6 0.0 7 0.0 8 1.0 9 1.0 10 0.0 11 0.0 12 1.0 13 0.0 14 57.0 15 127.0 16 242.0 17 445.0 18 623.0 19 797.0 20 839.0 21 784.0 22 664.0 23 614.0 24 556.0 25 476.0 26 459.0 27 510.0 28 467.0 29 509.0 30 528.0 31 539.0 32 584.0 33 674.0 34 980.0 35 913.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.990316333118145 26.396061975468044 13.718528082633958 24.89509360877986 2 14.122014202711428 21.32827630729503 41.59134925758554 22.958360232408005 3 14.606197546804388 26.694641704325374 30.92317624273725 27.77598450613299 4 9.627955774352353 18.602211282382374 40.190460818335886 31.579372124929385 5 11.59092743562838 37.05706675276455 38.61490031479538 12.737105496811688 6 26.570321330534476 43.419990311642174 18.359438075246246 11.650250282577103 7 26.626836751170675 32.464072339738415 24.132084611658325 16.777006297432585 8 24.24511545293073 35.031487162925885 22.129823994832876 18.59357338931051 9 23.476225074675064 14.628239283119399 22.927262452571245 38.968273189634296 10 14.258033263361861 26.376554174067497 34.70046827062813 24.664944291942515 11 30.143733850129202 25.920542635658915 25.35529715762274 18.580426356589147 12 19.418651594670973 29.931368591037543 30.536939846588616 20.113039967702868 13 23.63768466941148 23.04835714862356 29.603616694922096 23.710341487042868 14 19.336347489100596 22.17019215243016 29.000484417891165 29.49297594057807 15 22.559547840129188 29.051271699636654 24.263221639079532 24.125958821154622 16 21.92621296520546 29.91846290465811 26.996044239928956 21.159279890207475 17 18.280177634234963 30.246265643924104 28.905934598304402 22.567622123536534 18 19.102212175036332 28.556434684321008 30.46181172291297 21.879541417729694 19 20.713593800452053 28.245075879883757 29.65773329028092 21.383597029383274 20 19.80301929442157 30.33018487123597 29.143456849923304 20.723338984419147 21 21.76475337046904 28.320012916767578 28.49761847097764 21.417615241785743 22 19.491320145337102 28.60718611223254 30.536939846588616 21.364553895841745 23 20.502058277504236 28.743239970941964 29.534264266688194 21.220437484865606 24 18.690456967544 29.84014209591474 30.058130146940094 21.411270789601165 25 19.833710041976104 27.663868259606073 30.586051017113334 21.91637068130449 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 3.0 2 4.5 3 6.0 4 6.0 5 6.0 6 20.0 7 34.0 8 34.0 9 34.0 10 45.5 11 57.0 12 57.0 13 57.0 14 74.0 15 91.0 16 91.0 17 91.0 18 188.0 19 285.0 20 285.0 21 285.0 22 369.0 23 453.0 24 453.0 25 453.0 26 652.0 27 851.0 28 851.0 29 851.0 30 1078.0 31 1305.0 32 1305.0 33 1305.0 34 1394.0 35 1483.0 36 1483.0 37 1483.0 38 1520.0 39 1557.0 40 1557.0 41 1557.0 42 1418.5 43 1280.0 44 1280.0 45 1280.0 46 1414.5 47 1549.0 48 1549.0 49 1549.0 50 1333.0 51 1117.0 52 1117.0 53 1117.0 54 980.0 55 843.0 56 843.0 57 843.0 58 676.0 59 509.0 60 509.0 61 509.0 62 443.5 63 378.0 64 378.0 65 378.0 66 337.0 67 296.0 68 296.0 69 296.0 70 221.0 71 146.0 72 146.0 73 146.0 74 110.0 75 74.0 76 74.0 77 74.0 78 56.5 79 39.0 80 39.0 81 39.0 82 25.5 83 12.0 84 12.0 85 12.0 86 10.0 87 8.0 88 8.0 89 8.0 90 5.0 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 7.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.008069722401549385 5 0.02420916720464816 6 0.04841833440929632 7 0.04841833440929632 8 0.04841833440929632 9 0.040348612007746934 10 0.04841833440929632 11 0.06455777921239508 12 0.05648805681084571 13 0.040348612007746934 14 0.04841833440929632 15 0.05648805681084571 16 0.040348612007746934 17 0.05648805681084571 18 0.04841833440929632 19 0.03227888960619754 20 0.040348612007746934 21 0.040348612007746934 22 0.05648805681084571 23 0.02420916720464816 24 0.04841833440929632 25 0.03227888960619754 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 12392.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.64460942543577 #Duplication Level Percentage of deduplicated Percentage of total 1 77.60178033774055 47.83731439638477 2 11.899463280534102 14.670755326016785 3 4.084304228302134 7.553260167850226 4 2.1206964262338004 5.229180116204002 5 1.1650739625605446 3.591026468689477 6 0.798533839507789 2.9535183989670752 7 0.5367194658986778 2.3160103292446736 8 0.37963084173321116 1.8721755971594578 9 0.32726796701138894 1.815687540348612 >10 1.0210760570755335 9.506132989025177 >50 0.06545359340227778 2.654938670109748 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 81 0.6536475145255003 No Hit ATCCTATTCCCATCCTCAAAACGCC 76 0.6132989025177534 No Hit TATCAACGCAGAGTACTTTTTTTTT 63 0.5083925112976113 No Hit CTCCGAGGTCACCCCAACCGAAATT 55 0.4438347320852163 No Hit GGTATCAACGCAGAGTACTTTTTTT 54 0.43576500968366694 No Hit ATCCTAGCCCTAGCCCTACACAAAT 44 0.35506778566817304 No Hit CCATTGGGATGTCCTGATCCAACAT 36 0.2905100064557779 No Hit TGCTTACACCACATGAAACACTGTC 34 0.27437056165267915 No Hit AAGAGGGACAGCTCTTCTGGAACGG 30 0.24209167204648158 No Hit ATCCTGACCGTGCAAAGGTAGCATA 30 0.24209167204648158 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 28 0.22595222724338285 No Hit CATTTACACCTACTACCCAACTATC 25 0.20174306003873468 No Hit CTTTTAGGCCTACAATGGTTAAAAG 22 0.17753389283408652 No Hit ATTAATAAGTGTCCTGCAGTAATGT 21 0.16946417043253711 No Hit GTCCAACTGTCTCTTATCTTTAATC 21 0.16946417043253711 No Hit GTATTAGAGGCACTGCCTGCCCAGT 20 0.16139444803098774 No Hit CATCTAGCCTATCAGTTTACTCCAT 20 0.16139444803098774 No Hit CCCCTATACTATATCAATTTACCAG 19 0.15332472562943836 No Hit TCCTAACACTCCTCGTCCCCATTCT 19 0.15332472562943836 No Hit ATTCCATTCCATTCCATTCCATTCC 18 0.14525500322788895 No Hit GATTAAACCTTGTACCTTTTGCATA 17 0.13718528082633957 No Hit CCATAGGGTCTTCTCGTCTTATTAT 17 0.13718528082633957 No Hit CACTAGCATTAAGTCTATGAGTTCC 17 0.13718528082633957 No Hit GTGGTACTCCCGCTGTAAAAATTGG 16 0.12911555842479017 No Hit ATCCTATTTAAGAGTTCATATCGAC 16 0.12911555842479017 No Hit ATCCTACTCTCTACAAACACTTATT 16 0.12911555842479017 No Hit CTTCTACACCATTGGGATGTCCTGA 16 0.12911555842479017 No Hit CGTCTATGTGGCAAAATAGTGAGAA 16 0.12911555842479017 No Hit ACCAATACCACACCCATTAATTAAT 16 0.12911555842479017 No Hit CCAGAAGAGCTGTCCCTCTTTTGGC 16 0.12911555842479017 No Hit CTCCTAGGCCTTTTACCACATACAT 16 0.12911555842479017 No Hit GTATTGGAATTAGTGAAATTGGAGT 16 0.12911555842479017 No Hit AGCATGAACGGCTAAACGAGGGTCC 15 0.12104583602324079 No Hit GTTATATAATTTAAGCTCCATAGGG 15 0.12104583602324079 No Hit GTTATAGATTAACCCAATTTTAAGT 14 0.11297611362169142 No Hit ACCTATAACTTCTCTGTTAACCCAA 14 0.11297611362169142 No Hit GCTATCACCAAGCTCGTTAGGCTTT 13 0.10490639122014203 No Hit ATCCTGTCCGTGTCCGGGCCGGGTG 13 0.10490639122014203 No Hit TACTAGAGTAGCTCCTCCGATTAGG 13 0.10490639122014203 No Hit TACTAACAGTGTTGCATCTATAAAG 13 0.10490639122014203 No Hit TCTAGGAGCTATAGAACTAGTACCG 13 0.10490639122014203 No Hit CTCCTGGTGGTGCCCTTCCGTCAAT 13 0.10490639122014203 No Hit GTTAGTATGAGTAACAAGAATTCCA 13 0.10490639122014203 No Hit GCTGTAAGCCGGACTGCTAATGCCA 13 0.10490639122014203 No Hit GAGTAGGATCCATTTATTAATAGAA 13 0.10490639122014203 No Hit ACCTTGTACCTTTTGCATAATGAAC 13 0.10490639122014203 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 35 0.0020107967 16.285715 1 AACGCAG 40 0.00489731 14.25 6 ACGCAGA 40 0.00489731 14.25 7 TCAACGC 40 0.00489731 14.25 4 CAGAGTA 40 0.00489731 14.25 10 AGAGTAC 40 0.00489731 14.25 11 GCAGAGT 40 0.00489731 14.25 9 CGCAGAG 40 0.00489731 14.25 8 TATCAAC 40 0.00489731 14.25 2 CAACGCA 40 0.00489731 14.25 5 >>END_MODULE