FastQCFastQC Report
Thu 2 Feb 2017
SRR4062209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12392
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC630.5083925112976113No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA290.23402194964493223No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG270.21788250484183347No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT220.17753389283408652No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT220.17753389283408652No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC210.16946417043253711No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC210.16946417043253711No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA210.16946417043253711No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT190.15332472562943836No Hit
GAGTAGGATCCATTTATTAATAGAACTGATAAAAGGATAATAGCTATGGT190.15332472562943836No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC180.14525500322788895No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT180.14525500322788895No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA170.13718528082633957No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT160.12911555842479017No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC160.12911555842479017No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT160.12911555842479017No Hit
GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA150.12104583602324079No Hit
CAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTT150.12104583602324079No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT150.12104583602324079No Hit
ACCTCGGAGAATAAAAAATCCTCCGAATGATTATAACCTAGACTTACAAG150.12104583602324079No Hit
AAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGAC140.11297611362169142No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC140.11297611362169142No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT140.11297611362169142No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT140.11297611362169142No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT140.11297611362169142No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA140.11297611362169142No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA130.10490639122014203No Hit
GATAAAAGGATAATAGCTATGGTTACTTCATATGAAATTGTTTGGGCTAC130.10490639122014203No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG130.10490639122014203No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA130.10490639122014203No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA130.10490639122014203No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG130.10490639122014203No Hit
GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT130.10490639122014203No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC130.10490639122014203No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCA254.191585E-544.05
TCATTTA207.5575145E-444.038
CTTCCTT207.5575145E-444.044
TTACACC250.00226405435.24
Produced by FastQC (version 0.11.5)