##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062209_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12392
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.83795997417689	32.0	32.0	32.0	21.0	32.0
2	28.78010006455778	32.0	32.0	32.0	14.0	32.0
3	30.357569399612654	32.0	32.0	32.0	21.0	32.0
4	30.984828921885086	32.0	32.0	32.0	32.0	32.0
5	30.682779212395094	32.0	32.0	32.0	32.0	32.0
6	34.09796642995481	36.0	36.0	36.0	32.0	36.0
7	34.089816010329244	36.0	36.0	36.0	32.0	36.0
8	33.9452872821175	36.0	36.0	36.0	32.0	36.0
9	34.391623628147194	36.0	36.0	36.0	32.0	36.0
10	34.04979018721756	36.0	36.0	36.0	32.0	36.0
11	34.51299225306649	36.0	36.0	36.0	32.0	36.0
12	34.249677211103936	36.0	36.0	36.0	32.0	36.0
13	34.44795029051001	36.0	36.0	36.0	32.0	36.0
14	34.27743705616527	36.0	36.0	36.0	32.0	36.0
15	34.27872821174952	36.0	36.0	36.0	32.0	36.0
16	34.243867010974824	36.0	36.0	36.0	32.0	36.0
17	34.19932214331827	36.0	36.0	36.0	32.0	36.0
18	34.2249838605552	36.0	36.0	36.0	32.0	36.0
19	34.22449967721111	36.0	36.0	36.0	32.0	36.0
20	34.23950936087799	36.0	36.0	36.0	32.0	36.0
21	34.270012911555845	36.0	36.0	36.0	32.0	36.0
22	34.234021949644934	36.0	36.0	36.0	32.0	36.0
23	34.253712072304715	36.0	36.0	36.0	32.0	36.0
24	34.16268560361524	36.0	36.0	36.0	32.0	36.0
25	34.216429954809556	36.0	36.0	36.0	32.0	36.0
26	34.074241446094256	36.0	36.0	36.0	32.0	36.0
27	34.03244028405423	36.0	36.0	36.0	32.0	36.0
28	34.037201420271145	36.0	36.0	36.0	32.0	36.0
29	33.93963847643641	36.0	36.0	36.0	32.0	36.0
30	33.873143963847646	36.0	36.0	36.0	32.0	36.0
31	33.93253712072305	36.0	36.0	36.0	32.0	36.0
32	33.934715945771465	36.0	36.0	36.0	32.0	36.0
33	33.80108134280181	36.0	36.0	36.0	32.0	36.0
34	33.84602969657844	36.0	36.0	36.0	32.0	36.0
35	33.74354422207876	36.0	36.0	36.0	32.0	36.0
36	33.63129438347321	36.0	36.0	36.0	27.0	36.0
37	33.57755003227889	36.0	36.0	36.0	27.0	36.0
38	33.747256294383476	36.0	36.0	36.0	32.0	36.0
39	33.63742737249839	36.0	36.0	36.0	27.0	36.0
40	33.59619109102647	36.0	36.0	36.0	27.0	36.0
41	33.465945771465464	36.0	36.0	36.0	21.0	36.0
42	33.46901226597805	36.0	36.0	36.0	21.0	36.0
43	33.41228211749516	36.0	36.0	36.0	21.0	36.0
44	33.36499354422208	36.0	36.0	36.0	21.0	36.0
45	33.4083279535184	36.0	36.0	36.0	21.0	36.0
46	33.45698837959974	36.0	36.0	36.0	21.0	36.0
47	33.29381859264041	36.0	36.0	36.0	21.0	36.0
48	33.248789541639766	36.0	36.0	36.0	21.0	36.0
49	33.271061975468044	36.0	36.0	36.0	21.0	36.0
50	32.52275661717237	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	4.0
24	7.0
25	22.0
26	61.0
27	125.0
28	214.0
29	355.0
30	579.0
31	935.0
32	1437.0
33	2369.0
34	3364.0
35	2919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.80163021547898	24.71955451537406	15.55968041320313	21.91913485594383
2	17.34990316333118	24.75790832795352	38.02453195610071	19.86765655261459
3	18.25371207230471	27.5984506132989	32.56939961265333	21.578437701743063
4	12.22562943834732	21.070045190445448	42.019044544867654	24.68528082633957
5	16.15558424790187	36.789864428663655	35.07908327953518	11.97546804389929
6	27.784054228534536	44.79502905100065	17.34990316333118	10.071013557133634
7	26.533247256294384	34.36894770819884	23.01484828921885	16.082956746287927
8	24.176888315041964	36.96739832149774	19.7546804389929	19.101032924467397
9	24.273724983860557	14.703034215622981	22.74854744996772	38.27469335054874
10	14.509360877985797	27.041639767591995	33.63460296965785	24.814396384764365
11	32.14977404777276	26.226597805035507	24.89509360877986	16.72853453841188
12	20.166236281471917	28.615235635894127	29.72078760490639	21.497740477727568
13	24.168818592640413	23.224661071659135	28.58295674628793	24.023563589412525
14	20.004841833440928	21.97385409941898	28.486120077469334	29.535183989670756
15	23.983214977404778	29.42220787604906	22.942220787604906	23.65235635894125
16	23.474822466107163	30.059715945771465	24.967721110393803	21.497740477727568
17	19.908005164622335	30.737572627501613	26.993221433182697	22.36120077469335
18	20.198515171078117	30.14848289218851	28.53453841187863	21.118463524854743
19	21.263718528082634	29.510974822466107	28.873466752743703	20.351839896707553
20	21.683344092963203	29.89025177533893	27.953518398967077	20.472885732730795
21	23.781471917366044	27.832472562943835	26.912524209167206	21.473531310522915
22	21.13460296965784	28.244028405422856	29.32537120723047	21.29599741768883
23	22.385409941898	29.09134925758554	28.14719173660426	20.376049063912202
24	21.005487411233055	28.841187863137506	29.002582311168496	21.150742414460943
25	22.554874112330538	28.203679793415105	28.05842479018722	21.18302130406714
26	21.58650742414461	28.591026468689478	29.373789541639766	20.448676565526146
27	21.279857972885733	29.938670109748223	28.227888960619758	20.55358295674629
28	21.457391865719817	28.106843124596516	30.600387346675273	19.835377663008394
29	21.417043253712073	28.647514525500323	29.172046481601033	20.76339573918657
30	21.102324080051645	28.647514525500323	28.29244673983215	21.95771465461588
31	22.449967721110394	27.203034215622985	29.42220787604906	20.92479018721756
32	20.432537120723048	30.39864428663654	28.881536475145253	20.28728211749516
33	20.997417688831504	29.438347320852166	28.849257585539057	20.714977404777276
34	21.255648805681084	28.001936733376372	30.204970948999353	20.53744351194319
35	23.31342801807618	29.147837314396384	27.679147837314392	19.85958683021304
36	22.00613298902518	27.75984506132989	28.461910910264688	21.772111039380246
37	22.76468689477082	27.89703034215623	27.76791478373144	21.57036797934151
38	20.892511297611364	28.13105229180116	30.527759845061333	20.448676565526146
39	21.61071659134926	28.623305358295674	27.348289218850873	22.417688831504197
40	22.030342156229825	28.9057456423499	29.519044544867658	19.544867656552615
41	22.320852162685604	28.05035506778567	28.913815364751454	20.714977404777276
42	20.852162685603616	29.73692704970949	28.357004519044544	21.05390574564235
43	20.424467398321497	28.591026468689478	29.397998708844415	21.58650742414461
44	20.836023240800515	28.841187863137506	29.13169786959329	21.19109102646869
45	21.755971594577147	28.615235635894127	28.59909619109103	21.0296965784377
46	22.007908966185134	28.141392946493422	28.343152287950936	21.50754579937051
47	21.247579083279536	29.71271788250484	28.83311814073596	20.206584893479665
48	21.392834086507424	31.34280180761782	26.081342801807615	21.18302130406714
49	20.989347966429957	28.42156229825694	29.002582311168496	21.58650742414461
50	20.480955455132342	28.623305358295674	28.938024531956103	21.95771465461588
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	2.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	1.0
9	1.0
10	1.0
11	2.5
12	4.0
13	5.5
14	7.0
15	10.5
16	14.0
17	31.5
18	49.0
19	55.0
20	61.0
21	80.0
22	99.0
23	114.0
24	129.0
25	216.5
26	304.0
27	380.5
28	457.0
29	503.5
30	550.0
31	549.0
32	548.0
33	613.5
34	679.0
35	822.5
36	966.0
37	989.5
38	1013.0
39	1047.0
40	1081.0
41	1123.5
42	1166.0
43	958.0
44	750.0
45	719.5
46	689.0
47	641.0
48	593.0
49	513.0
50	433.0
51	432.5
52	432.0
53	383.0
54	334.0
55	336.0
56	338.0
57	342.5
58	347.0
59	303.0
60	259.0
61	263.5
62	268.0
63	236.0
64	204.0
65	197.0
66	190.0
67	146.0
68	102.0
69	113.5
70	125.0
71	97.5
72	70.0
73	58.0
74	46.0
75	40.5
76	35.0
77	28.5
78	22.0
79	14.5
80	7.0
81	5.0
82	3.0
83	3.5
84	4.0
85	2.0
86	0.0
87	1.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008069722401549385
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.008069722401549385
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	12392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.64912846998064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.08534148348419	56.35087153001936
2	8.992594334077818	12.346675274370561
3	3.691077935817562	7.601678502259522
4	1.95133419536852	5.358295674628793
5	1.0344422240507818	3.5506778566817303
6	0.6700364405783472	2.75984506132989
7	0.29387563183260845	1.4122014202711426
8	0.35265075819913017	1.9367333763718526
9	0.1528153285529564	0.9441575209812783
>10	0.7640766427647819	7.230471271788251
>50	0.011755025273304338	0.5083925112976113
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC	63	0.5083925112976113	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	29	0.23402194964493223	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	27	0.21788250484183347	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	22	0.17753389283408652	No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT	22	0.17753389283408652	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	21	0.16946417043253711	No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC	21	0.16946417043253711	No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA	21	0.16946417043253711	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	19	0.15332472562943836	No Hit
GAGTAGGATCCATTTATTAATAGAACTGATAAAAGGATAATAGCTATGGT	19	0.15332472562943836	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	18	0.14525500322788895	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	18	0.14525500322788895	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	17	0.13718528082633957	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	16	0.12911555842479017	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	16	0.12911555842479017	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	16	0.12911555842479017	No Hit
GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA	15	0.12104583602324079	No Hit
CAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTT	15	0.12104583602324079	No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT	15	0.12104583602324079	No Hit
ACCTCGGAGAATAAAAAATCCTCCGAATGATTATAACCTAGACTTACAAG	15	0.12104583602324079	No Hit
AAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGAC	14	0.11297611362169142	No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC	14	0.11297611362169142	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT	14	0.11297611362169142	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	14	0.11297611362169142	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	14	0.11297611362169142	No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA	14	0.11297611362169142	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	13	0.10490639122014203	No Hit
GATAAAAGGATAATAGCTATGGTTACTTCATATGAAATTGTTTGGGCTAC	13	0.10490639122014203	No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG	13	0.10490639122014203	No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA	13	0.10490639122014203	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	13	0.10490639122014203	No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG	13	0.10490639122014203	No Hit
GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT	13	0.10490639122014203	No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC	13	0.10490639122014203	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.008069722401549387	0.0
31	0.0	0.0	0.0	0.008069722401549387	0.0
32	0.0	0.0	0.0	0.008069722401549387	0.0
33	0.0	0.0	0.0	0.008069722401549387	0.0
34	0.0	0.0	0.0	0.016139444803098774	0.0
35	0.0	0.0	0.0	0.02420916720464816	0.0
36	0.0	0.0	0.0	0.03227888960619755	0.0
37	0.0	0.0	0.0	0.04841833440929632	0.0
38	0.0	0.0	0.0	0.056488056810845705	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCA	25	4.191585E-5	44.0	5
TCATTTA	20	7.5575145E-4	44.0	38
CTTCCTT	20	7.5575145E-4	44.0	44
TTACACC	25	0.002264054	35.2	4
>>END_MODULE