FastQCFastQC Report
Thu 2 Feb 2017
SRR4062208_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062208_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49290
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1930.39156015418949075No Hit
TATCAACGCAGAGTACTTTTTTTTT1580.3205518360722256No Hit
GGTATCAACGCAGAGTACTTTTTTT820.16636234530330696No Hit
GCGCAAGACGGACCAGAGCGAAAGC750.15216068167985392No Hit
ACGCAGAGTACTTTTTTTTTTTTTT650.13187259078920674No Hit
CATCTACACCGCGCAATGCGGTTCG640.12984378170014202No Hit
CTCCTGGAGCGTTCCAGGTTTGTCT620.12578616352201258No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT620.12578616352201258No Hit
ATATTATACTATGAACTCGAAGGCC590.11969973625481842No Hit
CTCCAGGAGCACCTCGACGCTTACA560.11361330898762426No Hit
GTATAGTAGGGGTGAAATGGAATTT520.10549807263136537No Hit
TCGTAGTTCCGACCATAAACGATGC520.10549807263136537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC250.005954932418.9979696
CGGTTCT350.00213182616.28397412
CCGCGGT400.005187278614.2484769
GTATCAA802.7396607E-511.873731
CAACGCA1150.00106464528.2599865
ATCAACG1200.00162163487.91581963
TCAACGC1100.00662454457.7718963
AACGCAG1350.00514003177.0362846
TATCAAC1350.00514003177.0362841
ACATGGG2008.363477E-46.174343
TACATGG2008.363477E-46.174342
GTACATG2050.00112629796.02374551
Produced by FastQC (version 0.11.5)