##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062208_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49290
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.309900588354637	32.0	32.0	32.0	32.0	32.0
2	31.007344288902413	32.0	32.0	32.0	32.0	32.0
3	30.98092919456279	32.0	32.0	32.0	32.0	32.0
4	30.963522012578615	32.0	32.0	32.0	32.0	32.0
5	30.94457293568675	32.0	32.0	32.0	32.0	32.0
6	34.62943801988233	36.0	36.0	36.0	32.0	36.0
7	34.52834246297424	36.0	36.0	36.0	32.0	36.0
8	34.5503144654088	36.0	36.0	36.0	32.0	36.0
9	34.6023939947251	36.0	36.0	36.0	32.0	36.0
10	34.37685128829377	36.0	36.0	36.0	32.0	36.0
11	34.539257455873404	36.0	36.0	36.0	32.0	36.0
12	34.474457293568676	36.0	36.0	36.0	32.0	36.0
13	34.522073442889024	36.0	36.0	36.0	32.0	36.0
14	34.42669912761209	36.0	36.0	36.0	32.0	36.0
15	34.438506796510445	36.0	36.0	36.0	32.0	36.0
16	34.450233313045246	36.0	36.0	36.0	32.0	36.0
17	34.395556908094946	36.0	36.0	36.0	32.0	36.0
18	34.38573747210388	36.0	36.0	36.0	32.0	36.0
19	34.395009129640904	36.0	36.0	36.0	32.0	36.0
20	34.345181578413474	36.0	36.0	36.0	32.0	36.0
21	34.36555082166768	36.0	36.0	36.0	32.0	36.0
22	34.27328058429702	36.0	36.0	36.0	32.0	36.0
23	34.27370663420572	36.0	36.0	36.0	32.0	36.0
24	34.25976871576385	36.0	36.0	36.0	32.0	36.0
25	33.98295800365186	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	2.0
6	10.0
7	2.0
8	10.0
9	7.0
10	6.0
11	2.0
12	2.0
13	4.0
14	17.0
15	6.0
16	25.0
17	32.0
18	46.0
19	50.0
20	69.0
21	104.0
22	127.0
23	169.0
24	218.0
25	335.0
26	466.0
27	558.0
28	749.0
29	1043.0
30	1411.0
31	1998.0
32	2809.0
33	4192.0
34	10019.0
35	24800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82004463379996	18.587948874010955	12.706431324812334	25.88557516737675
2	16.985189693649826	19.76262933657943	37.50253601136133	25.749644958409412
3	18.592006492189086	23.992696287279365	27.741935483870968	29.67336173666058
4	13.291807004017366	16.00860284867914	34.178062735868195	36.521527411435294
5	15.12826757590518	35.94739405747686	32.476863127131026	16.44747523948693
6	32.93140896819114	34.872013478675676	16.70422020583399	15.49235734729919
7	29.41869439818012	30.200674330747045	20.709265954421742	19.671365316651094
8	27.664716873832155	32.6041920545942	19.420749045413924	20.31034202615972
9	26.7915177120572	14.604322391940203	18.949057523561912	39.65510237244069
10	17.97440065014222	25.09752133279155	29.309223892726532	27.6188541243397
11	37.24199577441898	21.94254835039818	21.203071672354948	19.61238420282789
12	23.86252843678908	24.494231394215145	26.720425739356518	24.92281442963926
13	29.118758379718034	19.130134481777922	25.573883719985375	26.17722341851867
14	23.52522954416186	19.0277890631348	24.45559437718372	32.99138701551962
15	25.579505515714196	26.11177701481015	21.833289315970177	26.475428153505476
16	25.649515529464338	26.20609803164801	23.144894269637813	24.99949216924983
17	23.888358487883156	25.970464563570257	24.69479371914928	25.446383229397306
18	24.808578914231166	25.777362552551942	25.285862258057968	24.128196275158924
19	26.02506041713206	24.84108771145996	25.066509615970432	24.067342255437545
20	26.09976034769893	23.72557780575978	24.889313132133715	25.285348714407576
21	26.72935537592916	24.18254193915269	24.58873227994638	24.49937040497177
22	27.07368592087091	23.50718986107726	24.372410431391664	25.04671378666017
23	24.847709551656923	24.378654970760234	25.80206302794022	24.971572449642625
24	25.409170287942167	24.182674735003857	24.761401941274418	25.646753035779557
25	23.932422279529717	24.64109488902877	25.491908136536235	25.934574694905272
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	19.0
2	17.5
3	16.0
4	16.0
5	16.0
6	45.5
7	75.0
8	75.0
9	75.0
10	88.5
11	102.0
12	102.0
13	102.0
14	117.0
15	132.0
16	132.0
17	132.0
18	227.0
19	322.0
20	322.0
21	322.0
22	460.5
23	599.0
24	599.0
25	599.0
26	974.5
27	1350.0
28	1350.0
29	1350.0
30	1556.0
31	1762.0
32	1762.0
33	1762.0
34	2173.0
35	2584.0
36	2584.0
37	2584.0
38	3137.5
39	3691.0
40	3691.0
41	3691.0
42	4587.5
43	5484.0
44	5484.0
45	5484.0
46	6043.5
47	6603.0
48	6603.0
49	6603.0
50	6860.5
51	7118.0
52	7118.0
53	7118.0
54	6661.5
55	6205.0
56	6205.0
57	6205.0
58	5455.5
59	4706.0
60	4706.0
61	4706.0
62	4093.5
63	3481.0
64	3481.0
65	3481.0
66	2821.5
67	2162.0
68	2162.0
69	2162.0
70	1714.0
71	1266.0
72	1266.0
73	1266.0
74	1024.5
75	783.0
76	783.0
77	783.0
78	645.0
79	507.0
80	507.0
81	507.0
82	359.0
83	211.0
84	211.0
85	211.0
86	139.0
87	67.0
88	67.0
89	67.0
90	46.5
91	26.0
92	26.0
93	26.0
94	14.0
95	2.0
96	2.0
97	2.0
98	9.5
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.008115236356258876
5	0.03651856360316494
6	0.054777845404747415
7	0.11361330898762426
8	0.10955569080949483
9	0.1176709271657537
10	0.14201663623453034
11	0.13390139987827146
12	0.1176709271657537
13	0.12984378170014202
14	0.12578616352201258
15	0.13593020896733618
16	0.12375735443294787
17	0.12375735443294787
18	0.10752688172043011
19	0.09941164536417123
20	0.10549807263136537
21	0.10549807263136537
22	0.10955569080949483
23	0.0852099817407182
24	0.08926759991884763
25	0.08723879082978292
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	49290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.493000608642724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.61721529134726	24.14079935078109
2	15.4266245803898	12.49340637046054
3	7.966330978505937	9.67741935483871
4	4.604439100155319	7.4579022114019065
5	3.0963475124004205	6.269020085209982
6	2.0993035723232625	5.1004260499087035
7	1.6133072799238437	4.572935686751876
8	1.1473520717470815	3.716778251166565
9	0.8868179768525478	3.2318928788800974
>10	3.4821383836865576	21.351186853317103
>50	0.05010271055664111	1.2761209170217083
>100	0.010020542111328223	0.7121119902617163
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	193	0.39156015418949075	No Hit
TATCAACGCAGAGTACTTTTTTTTT	158	0.3205518360722256	No Hit
GGTATCAACGCAGAGTACTTTTTTT	82	0.16636234530330696	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	75	0.15216068167985392	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	65	0.13187259078920674	No Hit
CATCTACACCGCGCAATGCGGTTCG	64	0.12984378170014202	No Hit
CTCCTGGAGCGTTCCAGGTTTGTCT	62	0.12578616352201258	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	62	0.12578616352201258	No Hit
ATATTATACTATGAACTCGAAGGCC	59	0.11969973625481842	No Hit
CTCCAGGAGCACCTCGACGCTTACA	56	0.11361330898762426	No Hit
GTATAGTAGGGGTGAAATGGAATTT	52	0.10549807263136537	No Hit
TCGTAGTTCCGACCATAAACGATGC	52	0.10549807263136537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGC	25	0.0059549324	18.997969	6
CGGTTCT	35	0.002131826	16.283974	12
CCGCGGT	40	0.0051872786	14.248476	9
GTATCAA	80	2.7396607E-5	11.87373	1
CAACGCA	115	0.0010646452	8.259986	5
ATCAACG	120	0.0016216348	7.9158196	3
TCAACGC	110	0.0066245445	7.771896	3
AACGCAG	135	0.0051400317	7.036284	6
TATCAAC	135	0.0051400317	7.036284	1
ACATGGG	200	8.363477E-4	6.17434	3
TACATGG	200	8.363477E-4	6.17434	2
GTACATG	205	0.0011262979	6.0237455	1
>>END_MODULE