FastQCFastQC Report
Thu 2 Feb 2017
SRR4062208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49290
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.15013187259078922No Hit
CATCTACACCGCGCAATGCGGTTCGACAGCAATCTACACTCTCCTTCGCT710.14404544532359506No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT700.14201663623453034No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG670.13593020896733618No Hit
ATATTATACTATGAACTCGAAGGCCGATTCGGCCTGCTCGAGCCGCTCGT660.13390139987827146No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA570.11564211807668899No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.11361330898762426No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT540.10955569080949483No Hit
CTCCTGGAGCGTTCCAGGTTTGTCTCCTAGGTGCCTGCTTCTGAGCTGGT510.10346926354230068No Hit
CTCCAGGAGCACCTCGACGCTTACAAGAAACAGCGCGTGCACACACCAGA500.10144045445323595No Hit
ATCCAGAACTGTGTTTCTAAAATGCTTGGCTGGTTTGCCTTAATTCTAGC500.10144045445323595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATTC250.002320982335.23089640
GGTATCA451.0117474E-634.2174841
CTCCAAT300.00569620329.3292711
TTTGCCT300.00569620329.32927134
TGAGGCT300.00569620329.32927133
GTATCAA1250.028.1561011
AAGACGG500.002547829421.9969545
TCAACGC1600.021.9969544
TATCAAC1650.021.330382
CAACGCA1650.021.330385
ATCAACG1700.020.7030163
AACGCAG1700.020.7030166
AGACGGA550.00442742319.9972326
GAGTACT1102.6912421E-617.99750912
AGTACTT1102.6912421E-617.99750913
GTACTTT1154.1129388E-617.21500814
ACTTTTT1154.1129388E-617.21500816
ACGCAGA2055.456968E-1217.1683567
GCAGAGT2055.456968E-1217.1683569
CAGAGTA2055.456968E-1217.16835610
Produced by FastQC (version 0.11.5)