##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062208_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49290
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291499289916818	32.0	32.0	32.0	32.0	32.0
2	31.54386285250558	32.0	32.0	32.0	32.0	32.0
3	31.614607425441267	32.0	32.0	32.0	32.0	32.0
4	31.6856766078312	32.0	32.0	32.0	32.0	32.0
5	31.629661188882125	32.0	32.0	32.0	32.0	32.0
6	35.29819435991073	36.0	36.0	36.0	36.0	36.0
7	35.302393994725094	36.0	36.0	36.0	36.0	36.0
8	35.24211807668898	36.0	36.0	36.0	36.0	36.0
9	35.33012781497261	36.0	36.0	36.0	36.0	36.0
10	35.215784134712926	36.0	36.0	36.0	36.0	36.0
11	35.31008318117265	36.0	36.0	36.0	36.0	36.0
12	35.220247514708866	36.0	36.0	36.0	36.0	36.0
13	35.258348549401504	36.0	36.0	36.0	36.0	36.0
14	35.228626496246704	36.0	36.0	36.0	36.0	36.0
15	35.22444714952323	36.0	36.0	36.0	36.0	36.0
16	35.24434976668695	36.0	36.0	36.0	36.0	36.0
17	35.20588354635829	36.0	36.0	36.0	36.0	36.0
18	35.20180564008927	36.0	36.0	36.0	36.0	36.0
19	35.19646987218503	36.0	36.0	36.0	36.0	36.0
20	35.21022519780889	36.0	36.0	36.0	36.0	36.0
21	35.170866301481034	36.0	36.0	36.0	36.0	36.0
22	35.18689389328464	36.0	36.0	36.0	36.0	36.0
23	35.143964292960035	36.0	36.0	36.0	36.0	36.0
24	35.13304930006086	36.0	36.0	36.0	36.0	36.0
25	35.1132278352607	36.0	36.0	36.0	36.0	36.0
26	35.079772773382025	36.0	36.0	36.0	36.0	36.0
27	35.0618178129438	36.0	36.0	36.0	36.0	36.0
28	35.02937715560966	36.0	36.0	36.0	36.0	36.0
29	35.04656116859404	36.0	36.0	36.0	36.0	36.0
30	35.01442483262325	36.0	36.0	36.0	36.0	36.0
31	34.98924731182796	36.0	36.0	36.0	36.0	36.0
32	34.964698721850276	36.0	36.0	36.0	36.0	36.0
33	34.974457293568676	36.0	36.0	36.0	36.0	36.0
34	34.91625076080341	36.0	36.0	36.0	32.0	36.0
35	34.93696490160276	36.0	36.0	36.0	36.0	36.0
36	34.92432542097789	36.0	36.0	36.0	32.0	36.0
37	34.92286467843376	36.0	36.0	36.0	32.0	36.0
38	34.8943599107324	36.0	36.0	36.0	32.0	36.0
39	34.86895922093731	36.0	36.0	36.0	32.0	36.0
40	34.846175694867114	36.0	36.0	36.0	32.0	36.0
41	34.78383039156015	36.0	36.0	36.0	32.0	36.0
42	34.768553459119495	36.0	36.0	36.0	32.0	36.0
43	34.74629742341246	36.0	36.0	36.0	32.0	36.0
44	34.73323189287888	36.0	36.0	36.0	32.0	36.0
45	34.6362345303307	36.0	36.0	36.0	32.0	36.0
46	34.640210996145264	36.0	36.0	36.0	32.0	36.0
47	34.644025157232704	36.0	36.0	36.0	32.0	36.0
48	34.550050720227226	36.0	36.0	36.0	32.0	36.0
49	34.55220125786163	36.0	36.0	36.0	32.0	36.0
50	34.17561371474944	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	1.0
22	7.0
23	9.0
24	24.0
25	65.0
26	126.0
27	245.0
28	408.0
29	562.0
30	897.0
31	1414.0
32	1984.0
33	3370.0
34	7649.0
35	32527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6245509346648	19.42803791430717	13.01832795469768	25.92908319633035
2	16.67681071211199	19.82349360925137	37.679042402109964	25.820653276526677
3	18.107121119902615	24.39845810509231	27.666869547575573	29.8275512274295
4	13.067559342665854	16.4921890850071	33.6802596875634	36.75999188476364
5	14.826536822884966	36.16960843984581	32.6739703793873	16.329884357881923
6	33.274497869750455	35.02130249543518	16.65449381213228	15.049705822682085
7	30.282004463379998	29.894501927368633	20.251572327044027	19.571921282207345
8	27.770338811117874	32.46297423412457	19.269628727936702	20.497058226820855
9	26.796510448366806	14.86305538648813	19.034286873605193	39.306147291539865
10	17.869750456482045	24.71698113207547	29.22093730979915	28.192331101643337
11	37.47819030229255	21.827956989247312	21.113816189896532	19.580036518563602
12	23.578819233110167	24.32542097788598	26.701156421180766	25.394603367823088
13	29.245283018867923	19.26557110975857	25.266788395212007	26.222357476161495
14	23.77155609657131	18.94299046459728	24.65611685940353	32.62933657942788
15	26.198011767092716	25.89369040373301	22.071414079935078	25.8368837492392
16	25.71214088621977	26.038792403830545	23.214575555916248	25.034491154033432
17	24.323392168796918	25.18969364982755	24.777845404747413	25.70906877662812
18	24.958409413674172	25.69689592209373	25.029417731791437	24.315276932440657
19	26.001217285453436	24.382227632379795	25.5893690403733	24.027186041793467
20	26.764049502941774	23.268411442483263	24.719009941164536	25.248529113410427
21	27.425441265976875	24.075877459931018	24.35585311422195	24.142828159870156
22	27.20835869344695	23.033069588151754	24.968553459119498	24.7900182592818
23	25.23229864069791	23.96835057821059	25.897748021911138	24.90160275918036
24	25.522418340434168	23.751268005680664	25.177520795293162	25.548792858592005
25	24.309190505173465	23.956177723676202	25.49401501318726	26.240616757963075
26	24.816392777439646	24.520186650436194	25.325623858794888	25.337796713329276
27	25.267301020511674	23.788269187851245	24.267077846984115	26.677351944652965
28	24.707338351356288	24.36446265901114	25.934792752946905	24.99340623668567
29	25.468157195317414	24.565318833816878	25.198320111992533	24.76820385887318
30	25.111079551218324	24.244760494227922	24.782405810627118	25.861754143926635
31	24.87522824102252	24.814363968350577	25.345911949685533	24.964495840941368
32	25.529022702834304	23.67059587331859	24.68907869910122	26.11130272474589
33	25.1004260499087	23.552444714952323	25.104483668086836	26.242645567052143
34	24.938121322783527	23.64982755122743	25.713126394806245	25.6989247311828
35	25.74761614932035	23.542300669507	25.899776831000203	24.810306350172446
36	24.473524041387705	24.641915195780076	25.50821667681071	25.376344086021508
37	26.27563959504149	24.052019720424436	24.893992574408085	24.778348110125993
38	25.575167376749846	23.371880706025564	24.86305538648813	26.189896530736455
39	25.83485494015013	23.724893487522824	24.723067559342667	25.71718401298438
40	25.889632785554877	24.043416514505985	25.305335767904243	24.761614932034895
41	25.569080949482654	24.313248123351592	24.84885372286468	25.268817204301076
42	24.956886932175536	24.23055854247398	25.756253930897362	25.056300594453123
43	26.617566498265262	23.350985046766894	24.34921987542354	25.682228579544304
44	25.0684736959565	23.139037107671083	25.756253930897362	26.036235265475057
45	26.06922577503652	24.174241194611266	24.963479954552835	24.793053075799385
46	24.14485696895922	24.568878068573746	24.92189085007101	26.36437411239602
47	25.1232526527217	23.79232688835237	25.449897543062345	25.634522915863577
48	25.522936152082615	25.44786869281178	23.7030574773276	25.326137677778004
49	26.230040375758314	24.31269908900927	24.641386166737682	24.815874368494732
50	24.28281598701562	25.06390748630554	24.856968959220936	25.796307567457905
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	6.0
2	1.0
3	11.0
4	21.0
5	23.0
6	25.0
7	16.0
8	7.0
9	12.0
10	17.0
11	28.5
12	40.0
13	84.0
14	128.0
15	153.0
16	178.0
17	178.0
18	178.0
19	151.0
20	124.0
21	126.5
22	129.0
23	150.0
24	171.0
25	185.5
26	200.0
27	224.0
28	248.0
29	302.0
30	356.0
31	370.0
32	384.0
33	496.5
34	609.0
35	838.0
36	1067.0
37	1284.0
38	1501.0
39	1783.5
40	2066.0
41	2297.0
42	2528.0
43	2784.5
44	3041.0
45	3423.0
46	3805.0
47	3826.5
48	3848.0
49	3774.0
50	3700.0
51	3714.0
52	3728.0
53	3760.5
54	3793.0
55	3758.0
56	3723.0
57	3640.0
58	3557.0
59	3127.5
60	2698.0
61	2296.0
62	1894.0
63	1602.0
64	1310.0
65	1098.5
66	887.0
67	977.5
68	1068.0
69	962.5
70	857.0
71	715.5
72	574.0
73	443.5
74	313.0
75	228.0
76	143.0
77	146.5
78	150.0
79	123.5
80	97.0
81	84.5
82	72.0
83	48.0
84	24.0
85	19.0
86	14.0
87	8.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0426049908703591
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.004057618178129438
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.002028809089064719
28	0.002028809089064719
29	0.002028809089064719
30	0.002028809089064719
31	0.0
32	0.002028809089064719
33	0.0
34	0.0
35	0.0
36	0.0
37	0.002028809089064719
38	0.0
39	0.0
40	0.0
41	0.0
42	0.002028809089064719
43	0.006086427267194157
44	0.002028809089064719
45	0.004057618178129438
46	0.0
47	0.002028809089064719
48	0.002028809089064719
49	0.006086427267194157
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	49290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.23148711706228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.7008315701422	24.615540677622235
2	15.342223096983712	12.651653479407587
3	8.10411848644393	10.024345709068777
4	4.684347783299709	7.72570501115845
5	3.173744033853269	6.542909312233719
6	1.9731338877134283	4.881314668289714
7	1.5794912168479063	4.558734023128424
8	1.1661664124391085	3.8466220328667076
9	0.8217290754317769	3.0493000608642724
>10	3.409929636372583	20.95556908094948
>50	0.044284800472371204	1.148305944410631
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.15013187259078922	No Hit
CATCTACACCGCGCAATGCGGTTCGACAGCAATCTACACTCTCCTTCGCT	71	0.14404544532359506	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	70	0.14201663623453034	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	67	0.13593020896733618	No Hit
ATATTATACTATGAACTCGAAGGCCGATTCGGCCTGCTCGAGCCGCTCGT	66	0.13390139987827146	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	57	0.11564211807668899	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56	0.11361330898762426	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	54	0.10955569080949483	No Hit
CTCCTGGAGCGTTCCAGGTTTGTCTCCTAGGTGCCTGCTTCTGAGCTGGT	51	0.10346926354230068	No Hit
CTCCAGGAGCACCTCGACGCTTACAAGAAACAGCGCGTGCACACACCAGA	50	0.10144045445323595	No Hit
ATCCAGAACTGTGTTTCTAAAATGCTTGGCTGGTTTGCCTTAATTCTAGC	50	0.10144045445323595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.002028809089064719	0.0
30	0.0	0.0	0.0	0.006086427267194157	0.0
31	0.0	0.0	0.0	0.010144045445323595	0.0
32	0.0	0.0	0.0	0.016230472712517752	0.0
33	0.0	0.0	0.0	0.036518563603164945	0.0
34	0.0	0.0	0.0	0.0426049908703591	0.0
35	0.0	0.0	0.0	0.052749036315682694	0.0
36	0.0	0.0	0.0	0.07303712720632989	0.0
37	0.0	0.0	0.0	0.11969973625481842	0.0
38	0.0	0.0	0.0	0.1602759180361128	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATTC	25	0.0023209823	35.230896	40
GGTATCA	45	1.0117474E-6	34.217484	1
CTCCAAT	30	0.005696203	29.329271	1
TTTGCCT	30	0.005696203	29.329271	34
TGAGGCT	30	0.005696203	29.329271	33
GTATCAA	125	0.0	28.156101	1
AAGACGG	50	0.0025478294	21.996954	5
TCAACGC	160	0.0	21.996954	4
TATCAAC	165	0.0	21.33038	2
CAACGCA	165	0.0	21.33038	5
ATCAACG	170	0.0	20.703016	3
AACGCAG	170	0.0	20.703016	6
AGACGGA	55	0.004427423	19.997232	6
GAGTACT	110	2.6912421E-6	17.997509	12
AGTACTT	110	2.6912421E-6	17.997509	13
GTACTTT	115	4.1129388E-6	17.215008	14
ACTTTTT	115	4.1129388E-6	17.215008	16
ACGCAGA	205	5.456968E-12	17.168356	7
GCAGAGT	205	5.456968E-12	17.168356	9
CAGAGTA	205	5.456968E-12	17.168356	10
>>END_MODULE