FastQCFastQC Report
Thu 2 Feb 2017
SRR4062207_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062207_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698133
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11210.16057112326734305No Hit
TATCAACGCAGAGTACTTTTTTTTT8570.12275597916156376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA402.7547264E-416.6278889
AGCTCGT350.002166072816.2885428
ATCTTAA350.002173348316.2803671
TGGTCGA400.005271674614.25145210
GTTTTCG759.655232E-713.932754515
CCTAAGC759.663054E-713.9317553
GGTATCA3950.013.7043621
CGGCGCA707.241193E-613.57281110
GGCGCAA707.241193E-613.57281111
CGTTATT500.001499381113.2984932
GCGTTAT500.001501813513.2956341
ACCGGCG655.4352215E-513.156138
TCCGTCA751.4764477E-512.66614117
TTCTAGG751.4775369E-512.6652322
TTTTCGG853.936342E-612.29360716
GTATTAG701.09258224E-412.2102761
CTGGTCG550.00306154612.0930089
CCAAGAC1103.8058715E-812.08954053
CGCCAGT951.0375534E-611.99950118
GACCGGC802.8559725E-511.8770627
Produced by FastQC (version 0.11.5)