##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062207_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	698133
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20935982112291	32.0	32.0	32.0	32.0	32.0
2	30.722285008730427	32.0	32.0	32.0	32.0	32.0
3	30.75333496626001	32.0	32.0	32.0	32.0	32.0
4	30.764086499277358	32.0	32.0	32.0	32.0	32.0
5	30.680878858326423	32.0	32.0	32.0	32.0	32.0
6	34.33012477565163	36.0	36.0	36.0	32.0	36.0
7	34.25635086724163	36.0	36.0	36.0	32.0	36.0
8	34.21008174660129	36.0	36.0	36.0	32.0	36.0
9	34.3426209619084	36.0	36.0	36.0	32.0	36.0
10	34.00776499606808	36.0	36.0	36.0	32.0	36.0
11	34.32407148781106	36.0	36.0	36.0	32.0	36.0
12	34.12853568016409	36.0	36.0	36.0	32.0	36.0
13	34.232623296707075	36.0	36.0	36.0	32.0	36.0
14	34.124439039552634	36.0	36.0	36.0	32.0	36.0
15	34.086340281866065	36.0	36.0	36.0	32.0	36.0
16	34.07064986184581	36.0	36.0	36.0	32.0	36.0
17	34.02410428958379	36.0	36.0	36.0	32.0	36.0
18	34.01540250926399	36.0	36.0	36.0	32.0	36.0
19	34.023355148660784	36.0	36.0	36.0	32.0	36.0
20	33.99605232813805	36.0	36.0	36.0	32.0	36.0
21	33.98596112775073	36.0	36.0	36.0	32.0	36.0
22	33.956993867930606	36.0	36.0	36.0	32.0	36.0
23	33.91052707721881	36.0	36.0	36.0	32.0	36.0
24	33.89013411484631	36.0	36.0	36.0	32.0	36.0
25	33.53166946699268	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	15.0
5	49.0
6	137.0
7	40.0
8	135.0
9	93.0
10	52.0
11	16.0
12	26.0
13	34.0
14	126.0
15	155.0
16	268.0
17	365.0
18	509.0
19	681.0
20	1007.0
21	1504.0
22	2301.0
23	3349.0
24	4770.0
25	6514.0
26	8920.0
27	11230.0
28	14569.0
29	19221.0
30	24888.0
31	33418.0
32	47200.0
33	66603.0
34	143624.0
35	306313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.04309387833445	17.709098774291785	11.607630130334453	25.640177217039305
2	16.479858364690866	19.353101352038514	38.26029785240878	25.90674243086183
3	19.41933367615441	22.76054998431522	28.401914259399053	29.418202080131323
4	12.65842006116909	15.630492862412526	35.52462879531276	36.18645828110563
5	14.672256698222835	36.59975727494559	33.696370157140834	15.031615869690748
6	34.85257189377609	35.09092785396505	16.39223960005504	13.664260652203824
7	30.49613761477099	30.228846504064578	20.0745088310746	19.200507050089836
8	27.747887012908794	34.33880066651944	18.86674486702593	19.046567453545833
9	26.69875343479483	14.790843224203904	18.69876490801347	39.8116384329878
10	16.295902106107178	27.23839331691441	31.275182417885055	25.190522159093355
11	37.26966011688055	21.346191914758105	21.72718691993654	19.656961048424808
12	23.862117239361304	23.979448400107863	29.1203605423052	23.038073818225634
13	29.723209496189074	19.693175331250735	24.807445719055774	25.776169453504416
14	22.92651726031955	20.049677825533884	25.30442726371841	31.719377650428154
15	24.984687942418056	27.745448338480383	22.02770370774123	25.242160011360326
16	25.331543493257264	26.18630686291804	24.145487609516554	24.336662034308137
17	23.634110590644802	26.49726492245116	25.443679202797327	24.42494528410672
18	24.170573295722615	25.658701397782075	26.38390750861514	23.78681779788017
19	25.23998308110317	25.412469800486058	25.21145036526178	24.136096753148994
20	25.297722313186956	25.161227421708997	25.098858432430728	24.44219183267332
21	26.04425633923169	24.91146724933403	24.65210571742353	24.392170694010744
22	25.300150989605662	24.978814136527298	25.286385555471114	24.434649318395927
23	24.32351452750312	24.95831774280721	25.55584705658035	25.162320673109317
24	24.454608017434193	25.38208980902678	25.619515971784136	24.543786201754887
25	24.518952987072197	25.242261885133672	25.4517275541263	24.787057573667827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	363.0
1	363.0
2	317.0
3	271.0
4	271.0
5	271.0
6	559.0
7	847.0
8	847.0
9	847.0
10	1042.5
11	1238.0
12	1238.0
13	1238.0
14	1688.0
15	2138.0
16	2138.0
17	2138.0
18	3552.5
19	4967.0
20	4967.0
21	4967.0
22	7807.0
23	10647.0
24	10647.0
25	10647.0
26	15733.0
27	20819.0
28	20819.0
29	20819.0
30	26555.5
31	32292.0
32	32292.0
33	32292.0
34	39012.0
35	45732.0
36	45732.0
37	45732.0
38	52419.0
39	59106.0
40	59106.0
41	59106.0
42	66587.5
43	74069.0
44	74069.0
45	74069.0
46	81327.0
47	88585.0
48	88585.0
49	88585.0
50	90935.0
51	93285.0
52	93285.0
53	93285.0
54	87054.0
55	80823.0
56	80823.0
57	80823.0
58	74091.0
59	67359.0
60	67359.0
61	67359.0
62	58984.5
63	50610.0
64	50610.0
65	50610.0
66	41250.0
67	31890.0
68	31890.0
69	31890.0
70	24022.0
71	16154.0
72	16154.0
73	16154.0
74	12266.0
75	8378.0
76	8378.0
77	8378.0
78	6697.0
79	5016.0
80	5016.0
81	5016.0
82	3482.5
83	1949.0
84	1949.0
85	1949.0
86	1432.5
87	916.0
88	916.0
89	916.0
90	622.0
91	328.0
92	328.0
93	328.0
94	205.5
95	83.0
96	83.0
97	83.0
98	175.5
99	268.0
100	268.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.594350933131652E-4
2	2.8647836443772176E-4
3	5.729567288754435E-4
4	0.009740264390882538
5	0.032085576817024834
6	0.0625955226296422
7	0.10972121357964743
8	0.11158332294849262
9	0.12275597916156376
10	0.13894200675229504
11	0.14481481322326833
12	0.1376528541123253
13	0.13278272191688403
14	0.12032091306384313
15	0.13894200675229504
16	0.1237586534370958
17	0.1281990680858805
18	0.11659669432615276
19	0.09854855736657628
20	0.09611349126885564
21	0.09324870762447843
22	0.10513755974864387
23	0.08508407423800336
24	0.09353518598891615
25	0.0922460333489464
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	698133.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.405924055553186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27736748704082	49.473773747204504
2	13.503927504960158	16.854501486524786
3	3.665390275740675	6.862262015455071
4	1.3627050664053755	3.4016347553684567
5	0.6215161455435676	1.939314468904601
6	0.34690204582258843	1.2989245635792288
7	0.21623305333033122	0.9445956453103121
8	0.15142292759241305	0.7559750175681326
9	0.11448983182939432	0.6430359375250414
>10	0.6149004357696719	7.201069309914253
>50	0.06903787404652031	3.045107133939047
>100	0.053336618490830046	6.477791705658979
>500	0.002539838975454139	0.9404872866843016
>1k	2.3089445231401262E-4	0.16152692636327595
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1121	0.16057112326734305	No Hit
TATCAACGCAGAGTACTTTTTTTTT	857	0.12275597916156376	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4323918221886088E-4
2	0.0	0.0	0.0	0.0	1.4323918221886088E-4
3	0.0	0.0	0.0	0.0	1.4323918221886088E-4
4	0.0	0.0	0.0	0.0	1.4323918221886088E-4
5	0.0	0.0	0.0	0.0	2.8647836443772176E-4
6	0.0	0.0	0.0	0.0	2.8647836443772176E-4
7	0.0	0.0	0.0	0.0	2.8647836443772176E-4
8	0.0	0.0	0.0	0.0	2.8647836443772176E-4
9	0.0	0.0	0.0	0.0	2.8647836443772176E-4
10	0.0	0.0	0.0	0.0	2.8647836443772176E-4
11	0.0	0.0	0.0	0.0	2.8647836443772176E-4
12	0.0	0.0	0.0	0.0	4.297175466565826E-4
13	0.0	0.0	0.0	0.0	5.729567288754435E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	40	2.7547264E-4	16.627888	9
AGCTCGT	35	0.0021660728	16.288542	8
ATCTTAA	35	0.0021733483	16.280367	1
TGGTCGA	40	0.0052716746	14.251452	10
GTTTTCG	75	9.655232E-7	13.9327545	15
CCTAAGC	75	9.663054E-7	13.931755	3
GGTATCA	395	0.0	13.704362	1
CGGCGCA	70	7.241193E-6	13.572811	10
GGCGCAA	70	7.241193E-6	13.572811	11
CGTTATT	50	0.0014993811	13.298493	2
GCGTTAT	50	0.0015018135	13.295634	1
ACCGGCG	65	5.4352215E-5	13.15613	8
TCCGTCA	75	1.4764477E-5	12.666141	17
TTCTAGG	75	1.4775369E-5	12.665232	2
TTTTCGG	85	3.936342E-6	12.293607	16
GTATTAG	70	1.09258224E-4	12.210276	1
CTGGTCG	55	0.003061546	12.093008	9
CCAAGAC	110	3.8058715E-8	12.0895405	3
CGCCAGT	95	1.0375534E-6	11.999501	18
GACCGGC	80	2.8559725E-5	11.877062	7
>>END_MODULE