FastQCFastQC Report
Thu 2 Feb 2017
SRR4062207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698133
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7030.10069714509985919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACG250.002352396335.199149
TACACCG703.587047E-828.2850235
CTAGCGG1300.027.07626329
GTTATAG1550.025.562411
CGCAATA1500.023.46609136
GGTATCA4800.022.9292451
GTATCAA8600.022.779941
TCTAGCG1550.022.70912428
GACGGAC3100.021.9994627
ACGGACC3150.021.6502678
AAGACGG3050.021.6388175
AGCGAAA3050.021.63881717
GAGCGAA3100.021.29132816
CGGACCA3100.021.2898039
CGCAAGA3000.021.2661462
AGACGGA3450.021.0429656
GCGCAAG3150.020.9638821
GTATTAC956.821683E-720.8535461
AGCGGCG1800.020.77727131
TAGCGGC1700.020.70537830
Produced by FastQC (version 0.11.5)