##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062207_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	698133
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25898217101899	32.0	32.0	32.0	32.0	32.0
2	31.42589306049134	32.0	32.0	32.0	32.0	32.0
3	31.569895707551428	32.0	32.0	32.0	32.0	32.0
4	31.64946937044947	32.0	32.0	32.0	32.0	32.0
5	31.624597318848988	32.0	32.0	32.0	32.0	32.0
6	35.25237168275959	36.0	36.0	36.0	36.0	36.0
7	35.2577689351456	36.0	36.0	36.0	36.0	36.0
8	35.214397543161546	36.0	36.0	36.0	36.0	36.0
9	35.30172904016857	36.0	36.0	36.0	36.0	36.0
10	35.175783124418984	36.0	36.0	36.0	36.0	36.0
11	35.29827554348527	36.0	36.0	36.0	36.0	36.0
12	35.222586527208996	36.0	36.0	36.0	36.0	36.0
13	35.25545562235276	36.0	36.0	36.0	36.0	36.0
14	35.21474561437434	36.0	36.0	36.0	36.0	36.0
15	35.20317618562652	36.0	36.0	36.0	36.0	36.0
16	35.20856341126977	36.0	36.0	36.0	36.0	36.0
17	35.193408705791015	36.0	36.0	36.0	36.0	36.0
18	35.190992260786985	36.0	36.0	36.0	36.0	36.0
19	35.173445460965176	36.0	36.0	36.0	36.0	36.0
20	35.172427030379595	36.0	36.0	36.0	36.0	36.0
21	35.15745996822955	36.0	36.0	36.0	36.0	36.0
22	35.14346979730223	36.0	36.0	36.0	36.0	36.0
23	35.11461426404424	36.0	36.0	36.0	36.0	36.0
24	35.103447337398464	36.0	36.0	36.0	36.0	36.0
25	35.09138516586381	36.0	36.0	36.0	36.0	36.0
26	35.03808729855199	36.0	36.0	36.0	36.0	36.0
27	35.03320857200562	36.0	36.0	36.0	36.0	36.0
28	35.00586850929551	36.0	36.0	36.0	36.0	36.0
29	34.98829449402908	36.0	36.0	36.0	36.0	36.0
30	34.96274062392123	36.0	36.0	36.0	36.0	36.0
31	34.96626574019564	36.0	36.0	36.0	36.0	36.0
32	34.94460081388503	36.0	36.0	36.0	36.0	36.0
33	34.917187699191985	36.0	36.0	36.0	32.0	36.0
34	34.915450207911675	36.0	36.0	36.0	36.0	36.0
35	34.891686827581566	36.0	36.0	36.0	32.0	36.0
36	34.867813153081144	36.0	36.0	36.0	32.0	36.0
37	34.84801176853121	36.0	36.0	36.0	32.0	36.0
38	34.834124729815095	36.0	36.0	36.0	32.0	36.0
39	34.8127505790444	36.0	36.0	36.0	32.0	36.0
40	34.79899961755138	36.0	36.0	36.0	32.0	36.0
41	34.779024913590966	36.0	36.0	36.0	32.0	36.0
42	34.73754571120403	36.0	36.0	36.0	32.0	36.0
43	34.734072161035215	36.0	36.0	36.0	32.0	36.0
44	34.680016558449466	36.0	36.0	36.0	32.0	36.0
45	34.672907597835945	36.0	36.0	36.0	32.0	36.0
46	34.63386632633037	36.0	36.0	36.0	32.0	36.0
47	34.60614381500373	36.0	36.0	36.0	32.0	36.0
48	34.58497736104725	36.0	36.0	36.0	32.0	36.0
49	34.55327996241404	36.0	36.0	36.0	32.0	36.0
50	34.134492997752574	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	26.0
22	45.0
23	153.0
24	359.0
25	770.0
26	1741.0
27	3426.0
28	5664.0
29	8807.0
30	13226.0
31	19563.0
32	29416.0
33	50353.0
34	114611.0
35	449963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.76444526596484	18.21024139803666	11.953981484267645	26.07133185173085
2	15.852268006680973	19.850967337151303	38.09701161148371	26.199753044684016
3	18.839390202153456	23.282239917035866	28.603002579737673	29.275367301073
4	12.440770513312668	16.044673500140373	35.533967788326564	35.980588198220396
5	14.284240968411464	36.987364871736474	33.518111878395665	15.2102822814564
6	34.975713796654794	35.31791220297565	16.261228161396183	13.445145838973376
7	30.485881630004595	30.239796714952593	20.10677048642594	19.167551168616868
8	27.730962438389245	34.458620348844704	18.779229745621535	19.031187467144512
9	26.73244209914157	14.432636761190205	18.694288910565753	40.14063222910248
10	16.299616262230835	27.233922476089802	31.329273934909253	25.137187326770118
11	37.402328782624515	21.174905068231983	21.76762880425363	19.65513734488987
12	23.77555566059762	23.886995744363894	29.293415438032582	23.0440331570059
13	29.863220904899208	19.553867243061134	24.97690268186721	25.606009170172445
14	23.069672970623074	19.783766130522405	25.357775667387163	31.788785231467358
15	25.114555535979534	27.644589211511274	22.048377601402596	25.192477651106593
16	25.607944640762266	25.912758493749738	24.203193956435108	24.276102909052884
17	23.81766798016997	26.486500423271785	25.36149988612485	24.3343317104334
18	24.48691651435545	25.390184091260675	26.45660133040743	23.66629806397644
19	25.48053164654872	25.126587627285918	25.399744747777287	23.99313597838807
20	25.488949049603942	24.840502485210493	25.211923280764324	24.45862518442124
21	26.249726055064006	24.609350940293613	24.797853704093633	24.343069300548752
22	25.455178311295985	24.826501540537404	25.33027374440114	24.388046403765472
23	24.445771794199672	24.73797972592615	25.688514939130506	25.12773354074367
24	24.619340237491585	25.193015627461936	25.608124561328122	24.57951957371836
25	24.64755847764743	24.85582914356925	25.753512956039703	24.743099422743615
26	24.15291200598172	25.820373656942074	26.010596925774248	24.016117411301956
27	24.718891896924642	25.200607336742443	25.59594917851976	24.484551587813158
28	24.36332487727737	25.277209186399453	25.800758896405913	24.558707039917262
29	24.25203865750124	25.173355716906997	25.71007447026418	24.864531155327583
30	24.350185211180854	25.473484577194245	25.769134907652248	24.40719530397266
31	24.81923189310904	25.19623796072949	25.2490932946778	24.735436851483676
32	24.68075292890835	25.295684023107544	25.184099741164573	24.839463306819535
33	24.45654976422797	24.901308062085683	25.731237072645285	24.910905101041063
34	24.938764986463838	25.163364989328635	25.702089868649104	24.19578015555842
35	25.142809669231607	25.02821815931658	25.867600969444176	23.96137120200764
36	24.207708273351926	25.588390750759526	25.593404122137187	24.610496853751364
37	25.015398366483986	25.313195612253374	25.40658849548649	24.26481752577615
38	24.513667166571413	25.163113618751726	25.644970227735975	24.678248986940883
39	25.218011596727248	24.98137877294708	25.312836614489033	24.487773015836638
40	25.464775336504648	25.135754934947922	25.350040751547343	24.049428977000083
41	24.366735039992665	25.41425068182339	26.104668484862376	24.11434579332157
42	25.303358683055887	25.555327953978008	25.439442143758566	23.701871219207536
43	24.73328214795249	24.953159646820872	25.75761048307158	24.555947722155057
44	24.297417429806202	25.544081604003992	25.380925164338215	24.77757580185159
45	24.308215469502464	25.774148358023385	25.416904208719966	24.500731963754184
46	24.33328654139797	25.338549201720035	25.28483396979915	25.04333028708285
47	24.663960397174172	25.291062435184173	25.603324299702635	24.441652867939016
48	25.162045359808737	25.528321589670348	24.732884787002565	24.576748263518343
49	24.615322911026503	25.660991408329252	25.04848750497767	24.675198175666576
50	24.7528383128547	25.93887063366785	24.750689703577862	24.55760134989959
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	84.0
2	37.0
3	91.0
4	145.0
5	150.5
6	156.0
7	110.5
8	65.0
9	112.0
10	159.0
11	273.5
12	388.0
13	705.0
14	1022.0
15	1231.5
16	1441.0
17	1557.0
18	1673.0
19	1631.0
20	1589.0
21	1890.0
22	2191.0
23	2617.0
24	3043.0
25	3943.5
26	4844.0
27	6288.0
28	7732.0
29	9102.0
30	10472.0
31	11954.0
32	13436.0
33	15318.5
34	17201.0
35	19645.5
36	22090.0
37	25276.0
38	28462.0
39	30257.5
40	32053.0
41	34267.5
42	36482.0
43	37513.5
44	38545.0
45	41891.5
46	45238.0
47	47623.0
48	50008.0
49	51839.0
50	53670.0
51	51998.0
52	50326.0
53	49987.0
54	49648.0
55	49196.5
56	48745.0
57	46931.0
58	45117.0
59	41112.0
60	37107.0
61	32725.5
62	28344.0
63	24627.0
64	20910.0
65	17433.5
66	13957.0
67	12127.0
68	10297.0
69	9248.0
70	8199.0
71	6442.5
72	4686.0
73	4086.0
74	3486.0
75	2592.0
76	1698.0
77	1531.5
78	1365.0
79	1087.5
80	810.0
81	658.0
82	506.0
83	423.0
84	340.0
85	268.5
86	197.0
87	130.0
88	63.0
89	43.0
90	23.0
91	16.5
92	10.0
93	8.5
94	7.0
95	7.5
96	8.0
97	6.5
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03437740373252661
2	0.004440414648784688
3	0.0
4	1.4323918221886088E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.8647836443772176E-4
17	0.0
18	1.4323918221886088E-4
19	0.0
20	4.297175466565826E-4
21	0.0
22	0.0
23	0.0
24	4.297175466565826E-4
25	4.297175466565826E-4
26	8.594350933131652E-4
27	4.297175466565826E-4
28	0.002005348551064052
29	2.8647836443772176E-4
30	0.0015756310044074697
31	1.4323918221886088E-4
32	8.594350933131652E-4
33	1.4323918221886088E-4
34	4.297175466565826E-4
35	1.4323918221886088E-4
36	0.0
37	0.001002674275532026
38	0.0
39	7.161959110943044E-4
40	0.0
41	7.161959110943044E-4
42	0.004583653831003548
43	0.0024350660977206346
44	0.004297175466565826
45	0.0015756310044074697
46	0.001002674275532026
47	1.4323918221886088E-4
48	0.003724218737690383
49	0.002148587733282913
50	0.001002674275532026
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	698133.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.88612510404382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81733083280281	50.35333857951251
2	14.0627510085803	17.968293404823587
3	3.67045921202018	7.03474249225234
4	1.3640728482145343	3.485813145282525
5	0.6197634684769548	1.9797143241017428
6	0.3520022594114932	1.3492836229001244
7	0.20276961844552277	0.906791564791693
8	0.1475904262308403	0.7543184347474091
9	0.09932971672739573	0.5711211638456073
>10	0.5620861803772392	6.825283455864274
>50	0.05497817092573615	2.4880006540091393
>100	0.04461307231683358	5.43703304685295
>500	0.0022531854701301887	0.8462661110159966
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	703	0.10069714509985919	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.4323918221886088E-4
13	0.0	0.0	0.0	0.0	5.729567288754435E-4
14	0.0	0.0	0.0	0.0	5.729567288754435E-4
15	0.0	0.0	0.0	2.8647836443772176E-4	8.594350933131652E-4
16	0.0	0.0	0.0	2.8647836443772176E-4	8.594350933131652E-4
17	0.0	0.0	0.0	2.8647836443772176E-4	8.594350933131652E-4
18	0.0	0.0	0.0	2.8647836443772176E-4	8.594350933131652E-4
19	0.0	0.0	0.0	2.8647836443772176E-4	8.594350933131652E-4
20	0.0	0.0	0.0	2.8647836443772176E-4	0.001002674275532026
21	0.0	0.0	0.0	2.8647836443772176E-4	0.001002674275532026
22	0.0	0.0	0.0	4.297175466565826E-4	0.001002674275532026
23	0.0	0.0	0.0	5.729567288754435E-4	0.001002674275532026
24	0.0	0.0	0.0	0.001002674275532026	0.001002674275532026
25	0.0	0.0	0.0	0.0015756310044074697	0.001002674275532026
26	0.0	0.0	0.0	0.002291826915501774	0.001145913457750887
27	0.0	0.0	0.0	0.002578305279939496	0.001145913457750887
28	0.0	0.0	0.0	0.004010697102128104	0.001145913457750887
29	0.0	0.0	0.0	0.005156610559878992	0.001145913457750887
30	0.0	0.0	0.0	0.007018719928724183	0.001145913457750887
31	0.0	0.0	0.0	0.012748287217478617	0.001145913457750887
32	0.0	0.0	0.0	0.018764332870670773	0.001145913457750887
33	0.0	0.0	0.0	0.0230615083372366	0.001145913457750887
34	0.0	0.0	0.0	0.028218118897115594	0.001289152639969748
35	0.0	0.0	0.0	0.033804447003651164	0.001289152639969748
36	0.0	0.0	0.0	0.0421123195723451	0.001289152639969748
37	0.0	0.0	0.0	0.052568779874321944	0.001289152639969748
38	0.0	0.0	0.0	0.0670359372784269	0.001289152639969748
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTACG	25	0.0023523963	35.19914	9
TACACCG	70	3.587047E-8	28.285023	5
CTAGCGG	130	0.0	27.076263	29
GTTATAG	155	0.0	25.56241	1
CGCAATA	150	0.0	23.466091	36
GGTATCA	480	0.0	22.929245	1
GTATCAA	860	0.0	22.77994	1
TCTAGCG	155	0.0	22.709124	28
GACGGAC	310	0.0	21.999462	7
ACGGACC	315	0.0	21.650267	8
AAGACGG	305	0.0	21.638817	5
AGCGAAA	305	0.0	21.638817	17
GAGCGAA	310	0.0	21.291328	16
CGGACCA	310	0.0	21.289803	9
CGCAAGA	300	0.0	21.266146	2
AGACGGA	345	0.0	21.042965	6
GCGCAAG	315	0.0	20.963882	1
GTATTAC	95	6.821683E-7	20.853546	1
AGCGGCG	180	0.0	20.777271	31
TAGCGGC	170	0.0	20.705378	30
>>END_MODULE