FastQCFastQC Report
Thu 2 Feb 2017
SRR4062206_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062206_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259571
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19850.15759333931949845No Hit
GCGCAAGACGGACCAGAGCGAAAGC13440.10670299649642616No Hit
TATCAACGCAGAGTACTTTTTTTTT12750.10122494087272571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA400.005271692814.25271610
CGCTACA400.005288409514.24591618
CGTCGTA2400.013.85680610
CCGTCGT2350.013.7467539
CGTTATT802.0040843E-613.0592752
TAGGACG604.0886042E-412.6675684
GTTCTAG905.431484E-712.6610221
GGTATCA5250.012.480151
GTATTAG1301.9826984E-1012.4175411
ATACCGT2850.012.3361736
CGACCAT2700.012.31716110
TACCGTC2700.012.3171617
CTTATAC701.092287E-412.2122413
ACCGTCG2650.012.1905168
GCTAGTC550.003063511812.0932127
GTTATTC951.0413405E-611.9979913
AACCGCG907.441482E-611.6133237
CGGACCA2300.011.5669619
GTATCAA11000.011.4812451
TTAGGAC752.0764407E-411.3980913
Produced by FastQC (version 0.11.5)