##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062206_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1259571
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.239928515343717	32.0	32.0	32.0	32.0	32.0
2	30.748850997680957	32.0	32.0	32.0	32.0	32.0
3	30.778132395871292	32.0	32.0	32.0	32.0	32.0
4	30.78561907189035	32.0	32.0	32.0	32.0	32.0
5	30.695883757247508	32.0	32.0	32.0	32.0	32.0
6	34.3471936079824	36.0	36.0	36.0	32.0	36.0
7	34.28563693511521	36.0	36.0	36.0	32.0	36.0
8	34.23509115405166	36.0	36.0	36.0	32.0	36.0
9	34.37376852912619	36.0	36.0	36.0	32.0	36.0
10	34.04451674419306	36.0	36.0	36.0	32.0	36.0
11	34.366383475008554	36.0	36.0	36.0	32.0	36.0
12	34.16140574846515	36.0	36.0	36.0	32.0	36.0
13	34.26664793012859	36.0	36.0	36.0	32.0	36.0
14	34.165243563086165	36.0	36.0	36.0	32.0	36.0
15	34.11649124979854	36.0	36.0	36.0	32.0	36.0
16	34.110393935713034	36.0	36.0	36.0	32.0	36.0
17	34.04770274958696	36.0	36.0	36.0	32.0	36.0
18	34.05092289358837	36.0	36.0	36.0	32.0	36.0
19	34.054912347140416	36.0	36.0	36.0	32.0	36.0
20	34.04385461399159	36.0	36.0	36.0	32.0	36.0
21	34.03042385066026	36.0	36.0	36.0	32.0	36.0
22	33.99547464970216	36.0	36.0	36.0	32.0	36.0
23	33.96007608939869	36.0	36.0	36.0	32.0	36.0
24	33.93841871557856	36.0	36.0	36.0	32.0	36.0
25	33.58559938264695	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	18.0
5	88.0
6	276.0
7	68.0
8	231.0
9	158.0
10	100.0
11	30.0
12	56.0
13	54.0
14	211.0
15	308.0
16	479.0
17	650.0
18	883.0
19	1175.0
20	1764.0
21	2490.0
22	3727.0
23	5324.0
24	7851.0
25	11030.0
26	15352.0
27	19446.0
28	26087.0
29	34701.0
30	44661.0
31	60878.0
32	85788.0
33	120385.0
34	261071.0
35	554229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.397606469550524	17.21765185040514	11.526387744311117	25.858353935733213
2	17.058427035871738	19.006630035146888	37.12414782493404	26.81079510404733
3	19.513054883146353	22.190562312286684	27.94682617121598	30.349556633350982
4	13.294909214483761	15.164928873827666	34.36605761871036	37.174104292978214
5	15.398990263997439	35.822358258527046	33.070641016375454	15.708010461100066
6	35.6256375382523	34.012923953007274	16.232796397690404	14.128642111050027
7	31.49729334913372	29.81719078967761	19.47389796669361	19.21161789449506
8	28.555260336401478	33.07171600041011	18.57075073179998	19.802272931388437
9	26.925275044969784	14.763919568671621	18.48097120423634	39.829834182122255
10	16.898410941827816	26.4104998044285	30.406756915757978	26.28433233798571
11	37.32066546083361	21.30277672874719	21.498757726938443	19.87780008348075
12	24.62143045108747	23.416097762251884	28.056561037408166	23.90591074925248
13	29.508310100665618	19.210335734903534	24.834588970584143	26.4467651938467
14	23.536818108473103	19.388377211634076	24.581598854747337	32.49320582514548
15	25.322337781692774	26.948058659195674	21.70240777458536	26.027195784526196
16	26.05939445005286	25.89270526140076	23.04110394823653	25.00679634030985
17	24.378575738280404	26.07903131824061	24.623019705761006	24.91937323771798
18	24.989845952568874	25.215500282564683	25.38432374044109	24.410330024425352
19	25.67256555586991	25.106332590541648	24.950171499051113	24.270930354537334
20	25.78387991951403	24.418288624204916	24.65502608164939	25.14280537463166
21	26.535806750508982	24.276816323027607	24.408651026626003	24.77872589983741
22	26.08664898038985	24.230066776556836	24.62244461646795	25.06083962658537
23	24.706563817006426	24.380073355072877	25.158040771181756	25.75532205673894
24	24.857078375246942	24.86661443027634	25.04311091544539	25.23319627903133
25	24.990444435085333	24.476311337389713	24.892147272512	25.641096955012955
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	397.0
1	397.0
2	411.0
3	425.0
4	425.0
5	425.0
6	845.5
7	1266.0
8	1266.0
9	1266.0
10	1578.0
11	1890.0
12	1890.0
13	1890.0
14	2551.0
15	3212.0
16	3212.0
17	3212.0
18	5212.0
19	7212.0
20	7212.0
21	7212.0
22	11349.0
23	15486.0
24	15486.0
25	15486.0
26	22637.0
27	29788.0
28	29788.0
29	29788.0
30	37993.0
31	46198.0
32	46198.0
33	46198.0
34	58858.0
35	71518.0
36	71518.0
37	71518.0
38	85324.0
39	99130.0
40	99130.0
41	99130.0
42	114773.5
43	130417.0
44	130417.0
45	130417.0
46	146108.0
47	161799.0
48	161799.0
49	161799.0
50	167700.0
51	173601.0
52	173601.0
53	173601.0
54	164461.5
55	155322.0
56	155322.0
57	155322.0
58	143314.5
59	131307.0
60	131307.0
61	131307.0
62	115752.5
63	100198.0
64	100198.0
65	100198.0
66	81819.0
67	63440.0
68	63440.0
69	63440.0
70	48259.0
71	33078.0
72	33078.0
73	33078.0
74	25022.5
75	16967.0
76	16967.0
77	16967.0
78	13431.0
79	9895.0
80	9895.0
81	9895.0
82	6940.5
83	3986.0
84	3986.0
85	3986.0
86	2886.0
87	1786.0
88	1786.0
89	1786.0
90	1195.0
91	604.0
92	604.0
93	604.0
94	383.5
95	163.0
96	163.0
97	163.0
98	324.5
99	486.0
100	486.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.145289943957109E-4
2	0.0
3	3.175684419536493E-4
4	0.009447661148121067
5	0.0341386075100173
6	0.06533970693196335
7	0.10908475981107853
8	0.10892597559010171
9	0.11940573417457215
10	0.1364750379295808
11	0.14258823043718855
12	0.1384598406917911
13	0.13052062964294986
14	0.11972330261652579
15	0.1372689590344649
16	0.12234324226264338
17	0.1281388663282975
18	0.1152773444291747
19	0.09963709866295746
20	0.09646141424342097
21	0.0934445140448613
22	0.10678238860691458
23	0.08217083435550675
24	0.09439721937072225
25	0.09066579017776688
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1259571.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.92046426162134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.20722383465582	48.06054089308358
2	12.252054157495989	14.682975465513753
3	3.333506245235698	5.992357255006115
4	1.3836845450081927	3.316440813140848
5	0.7268549606312475	2.177674334594343
6	0.4638506270721608	1.6676486953324836
7	0.2976597978202488	1.2485139294186758
8	0.22440775624809345	1.0757293550635614
9	0.16402276510758	0.8845488211248954
>10	0.824673159872877	8.936585402280754
>50	0.06267074229425816	2.6636832503600103
>100	0.053265849774415265	6.4872326215058385
>500	0.005326572855239969	2.1822499944441844
>1k	7.989859282859954E-4	0.6238191691310334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1985	0.15759333931949845	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1344	0.10670299649642616	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1275	0.10122494087272571	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.939211048841233E-5	0.0
3	0.0	0.0	0.0	7.939211048841233E-5	0.0
4	0.0	0.0	0.0	7.939211048841233E-5	0.0
5	0.0	0.0	0.0	7.939211048841233E-5	0.0
6	0.0	0.0	0.0	7.939211048841233E-5	0.0
7	0.0	0.0	0.0	1.5878422097682465E-4	0.0
8	0.0	0.0	0.0	1.5878422097682465E-4	0.0
9	0.0	0.0	0.0	1.5878422097682465E-4	0.0
10	0.0	0.0	0.0	2.3817633146523698E-4	0.0
11	0.0	0.0	0.0	2.3817633146523698E-4	0.0
12	0.0	0.0	0.0	2.3817633146523698E-4	1.5878422097682465E-4
13	0.0	0.0	0.0	2.3817633146523698E-4	1.5878422097682465E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCCA	40	0.0052716928	14.252716	10
CGCTACA	40	0.0052884095	14.245916	18
CGTCGTA	240	0.0	13.856806	10
CCGTCGT	235	0.0	13.746753	9
CGTTATT	80	2.0040843E-6	13.059275	2
TAGGACG	60	4.0886042E-4	12.667568	4
GTTCTAG	90	5.431484E-7	12.661022	1
GGTATCA	525	0.0	12.48015	1
GTATTAG	130	1.9826984E-10	12.417541	1
ATACCGT	285	0.0	12.336173	6
CGACCAT	270	0.0	12.317161	10
TACCGTC	270	0.0	12.317161	7
CTTATAC	70	1.092287E-4	12.212241	3
ACCGTCG	265	0.0	12.190516	8
GCTAGTC	55	0.0030635118	12.093212	7
GTTATTC	95	1.0413405E-6	11.997991	3
AACCGCG	90	7.441482E-6	11.613323	7
CGGACCA	230	0.0	11.566961	9
GTATCAA	1100	0.0	11.481245	1
TTAGGAC	75	2.0764407E-4	11.398091	3
>>END_MODULE