FastQCFastQC Report
Thu 2 Feb 2017
SRR4062206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259571
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13650.10837023081668282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6450.027.9698981
GTATCAA14700.026.9396511
TCGTACG500.002579416622.00158741
CGTGCGT708.123976E-418.85700814
GTGCGTA708.123976E-418.85700815
TCAACGC21750.017.9026094
ATCAACG21850.017.8206733
GTAAACG2650.017.43452827
CAACGCA22450.017.3443975
TATCAAC22700.017.2509772
GTCCTAT3150.016.7624471
AACGCAG23400.016.7342576
TAAACGC2900.016.69019728
GTACTAG1206.4049855E-616.5005361
CGCAATA2400.016.4998836
TAGGACG800.001989036816.4992264
TACACAA2700.016.2955325
AACGCTT2850.016.2104130
CCTATTC3000.016.1325763
GTTACAC1958.931238E-1015.794133
Produced by FastQC (version 0.11.5)