##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1259571
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28458498965124	32.0	32.0	32.0	32.0	32.0
2	31.45448410609644	32.0	32.0	32.0	32.0	32.0
3	31.569036600556856	32.0	32.0	32.0	32.0	32.0
4	31.64749902943145	32.0	32.0	32.0	32.0	32.0
5	31.618826568728558	32.0	32.0	32.0	32.0	32.0
6	35.25686047074758	36.0	36.0	36.0	36.0	36.0
7	35.264632958364395	36.0	36.0	36.0	36.0	36.0
8	35.21703659420549	36.0	36.0	36.0	36.0	36.0
9	35.292627410443714	36.0	36.0	36.0	36.0	36.0
10	35.178711640709416	36.0	36.0	36.0	36.0	36.0
11	35.29802448611472	36.0	36.0	36.0	36.0	36.0
12	35.21359970974245	36.0	36.0	36.0	36.0	36.0
13	35.24492545477786	36.0	36.0	36.0	36.0	36.0
14	35.21198804989953	36.0	36.0	36.0	36.0	36.0
15	35.190623632967096	36.0	36.0	36.0	36.0	36.0
16	35.20491103717059	36.0	36.0	36.0	36.0	36.0
17	35.18650238851164	36.0	36.0	36.0	36.0	36.0
18	35.1781233451707	36.0	36.0	36.0	36.0	36.0
19	35.17904905717899	36.0	36.0	36.0	36.0	36.0
20	35.16837399400272	36.0	36.0	36.0	36.0	36.0
21	35.16458222680579	36.0	36.0	36.0	36.0	36.0
22	35.14788765381229	36.0	36.0	36.0	36.0	36.0
23	35.119840009018944	36.0	36.0	36.0	36.0	36.0
24	35.10330342632531	36.0	36.0	36.0	36.0	36.0
25	35.08286472140117	36.0	36.0	36.0	36.0	36.0
26	35.04666747646619	36.0	36.0	36.0	36.0	36.0
27	35.035358864248224	36.0	36.0	36.0	36.0	36.0
28	35.012407399027126	36.0	36.0	36.0	36.0	36.0
29	34.995736643666774	36.0	36.0	36.0	36.0	36.0
30	34.97626017112176	36.0	36.0	36.0	36.0	36.0
31	34.972513657427804	36.0	36.0	36.0	36.0	36.0
32	34.94692399237518	36.0	36.0	36.0	36.0	36.0
33	34.934748418310676	36.0	36.0	36.0	36.0	36.0
34	34.91717497465407	36.0	36.0	36.0	32.0	36.0
35	34.90773684055921	36.0	36.0	36.0	32.0	36.0
36	34.886771765942534	36.0	36.0	36.0	32.0	36.0
37	34.87908899141057	36.0	36.0	36.0	32.0	36.0
38	34.86142345290579	36.0	36.0	36.0	32.0	36.0
39	34.8499973403643	36.0	36.0	36.0	32.0	36.0
40	34.83733112305698	36.0	36.0	36.0	32.0	36.0
41	34.80506140582786	36.0	36.0	36.0	32.0	36.0
42	34.778419001390155	36.0	36.0	36.0	32.0	36.0
43	34.77159286773036	36.0	36.0	36.0	32.0	36.0
44	34.72911888254016	36.0	36.0	36.0	32.0	36.0
45	34.716571753398576	36.0	36.0	36.0	32.0	36.0
46	34.69773835694852	36.0	36.0	36.0	32.0	36.0
47	34.680180791714	36.0	36.0	36.0	32.0	36.0
48	34.65372972226258	36.0	36.0	36.0	32.0	36.0
49	34.64579130513484	36.0	36.0	36.0	32.0	36.0
50	34.22792125255345	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	11.0
21	27.0
22	99.0
23	245.0
24	658.0
25	1505.0
26	3162.0
27	5787.0
28	9859.0
29	15925.0
30	23607.0
31	34800.0
32	52159.0
33	88626.0
34	201615.0
35	821483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00841821033613	17.754561518454544	11.979748644946095	26.25727162626323
2	16.450194796894365	19.448407603867196	36.84078896910483	27.260608630133614
3	18.842933870026556	22.605105730946796	28.303263428247057	30.248696970779594
4	12.897724701505512	15.520760639932169	34.70165635760112	36.879858300961196
5	15.029641044450848	36.17525332037654	32.84697726448132	15.948128370691292
6	35.74617308964112	34.189606428240815	16.012963184967532	14.05125729715053
7	31.479606945539395	29.834284847777536	19.525775045630617	19.160333161052453
8	28.657058633455357	33.034263253123484	18.60697015094822	19.701707962472938
9	27.068343110471737	14.39942647139383	18.436356505508623	40.095873912625805
10	16.875110652753992	26.405815948445937	30.477281550623186	26.24179184817688
11	37.66218815771401	21.101152694052182	21.45960807290736	19.77705107532644
12	24.67165407904755	23.35279233961404	28.01644369392436	23.959109887414048
13	29.62326061809934	19.1504091472414	25.002639787673743	26.223690446985522
14	23.76436104038597	19.10547321270496	24.715319739816174	32.41484600709289
15	25.429848734211884	26.742835457469248	21.740973712478294	26.08634209584057
16	26.112230988049028	25.686845358271082	23.111507721328746	25.089415932351137
17	24.447927111691204	25.906598357694804	24.65903073347989	24.986443797134104
18	25.106048179972674	25.030625555249454	25.48554307068529	24.377783194092583
19	25.80100685074521	24.901732415242968	25.06861463148961	24.22864610252221
20	25.96932120323889	24.2187989999738	24.71587458229693	25.096005214490376
21	26.548483570993614	24.147269189271586	24.509773565761677	24.794473673973123
22	26.06339777590942	24.040963153327603	24.890061775001172	25.00557729576181
23	24.767658804195083	24.099573664028203	25.394221837611248	25.73854569416547
24	24.924238995897753	24.634771224394687	25.13685410617894	25.304135673528627
25	25.2237475259355	24.187359624378853	25.16253601436049	25.426356835325155
26	24.86536997355432	25.03034782698444	25.315049743442763	24.789232456018475
27	25.449419603054697	24.635490393936337	24.802769836348784	25.112320166660183
28	25.138441971080216	24.42199732762337	25.25483246834393	25.184728232952487
29	24.92324718969992	24.75422031291805	25.294725234069276	25.027807263312752
30	24.869735740003414	24.794629849430947	25.364593050720295	24.97104135984534
31	25.356413295722408	24.552960034551667	24.644499719347106	25.446126950378822
32	25.308500342579677	24.54736213649041	24.68161526119021	25.4625222597397
33	24.903756761241343	24.357141212589386	25.07643487573924	25.66266715043003
34	25.482767408644758	24.474717778654625	25.212911482651496	24.82960333004912
35	25.769687885300446	24.31339609024992	25.26935801055758	24.647558013892056
36	24.79883960122804	24.798918993590654	24.978345733097168	25.423895672084136
37	25.652594698578618	24.550136596511926	24.91098004126874	24.886288663640716
38	24.98564987602922	24.365915061556674	25.31028421581634	25.338150846597767
39	25.56108923837629	24.40322366796633	24.73119560100003	25.30449149265735
40	25.735211222877645	24.463507387441748	25.00789952126519	24.793381868415413
41	24.812235422640995	24.913302782885395	25.33098965032122	24.943472144152384
42	25.871230977736598	24.759688482276058	24.897837335283295	24.47124320470405
43	25.475344340806096	24.077814511698218	25.0600234377233	25.386817709772387
44	24.960222563096064	24.685194536279244	24.91798384766115	25.436599052963537
45	25.153567115796392	24.928883161962197	24.83043506911612	25.08711465312529
46	25.072565944500546	24.622323634585214	24.93450010479983	25.370610316114405
47	25.178217420886867	24.621434361446127	25.236888842664523	24.96345937500248
48	25.766664152471385	25.115778692591988	24.32261624514198	24.794940909794644
49	25.12822385741825	25.07860162157527	24.473448531023028	25.319725989983453
50	25.199694176734404	25.165078666921787	24.579314374725598	25.05591278161821
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	226.0
1	141.5
2	57.0
3	151.5
4	246.0
5	257.0
6	268.0
7	206.5
8	145.0
9	195.0
10	245.0
11	443.0
12	641.0
13	1190.5
14	1740.0
15	2110.0
16	2480.0
17	2433.5
18	2387.0
19	2196.5
20	2006.0
21	2368.5
22	2731.0
23	3473.5
24	4216.0
25	5354.5
26	6493.0
27	7981.0
28	9469.0
29	11218.5
30	12968.0
31	15131.5
32	17295.0
33	20262.0
34	23229.0
35	26791.0
36	30353.0
37	36237.5
38	42122.0
39	47091.5
40	52061.0
41	57329.5
42	62598.0
43	65934.5
44	69271.0
45	75811.0
46	82351.0
47	87520.5
48	92690.0
49	96682.5
50	100675.0
51	99229.5
52	97784.0
53	96551.0
54	95318.0
55	95711.0
56	96104.0
57	92416.0
58	88728.0
59	81284.0
60	73840.0
61	65233.0
62	56626.0
63	48863.5
64	41101.0
65	35008.5
66	28916.0
67	24653.0
68	20390.0
69	18108.0
70	15826.0
71	12504.0
72	9182.0
73	8056.5
74	6931.0
75	5124.0
76	3317.0
77	2903.5
78	2490.0
79	2158.0
80	1826.0
81	1418.0
82	1010.0
83	821.0
84	632.0
85	496.0
86	360.0
87	239.5
88	119.0
89	83.0
90	47.0
91	28.5
92	10.0
93	14.0
94	18.0
95	15.0
96	12.0
97	12.5
98	13.0
99	10.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03143927575341128
2	0.004287173966374265
3	4.7635266293047395E-4
4	0.0
5	0.0
6	3.175684419536493E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.351368839072986E-4
17	0.0
18	1.5878422097682465E-4
19	0.0
20	3.175684419536493E-4
21	0.0
22	0.0
23	7.939211048841233E-5
24	0.0014290579887914219
25	3.175684419536493E-4
26	6.351368839072986E-4
27	1.5878422097682465E-4
28	0.0014290579887914219
29	6.351368839072986E-4
30	0.0012702737678145972
31	6.351368839072986E-4
32	7.939211048841233E-4
33	1.5878422097682465E-4
34	7.939211048841233E-4
35	1.5878422097682465E-4
36	3.175684419536493E-4
37	0.0017466264307450712
38	0.0
39	9.527053258609479E-4
40	7.939211048841233E-5
41	0.0011908816573261848
42	0.004763526629304739
43	0.005001702960769976
44	0.005001702960769976
45	0.0019054106517218958
46	0.0021435869831871327
47	0.0011114895468377726
48	0.00444595818735109
49	0.004366566076862678
50	0.0015878422097682465
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1259571.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.226813629788055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.24230188751066	49.12980462891806
2	12.37659382485717	15.155588069722315
3	3.3136238731829604	6.086478939677687
4	1.3522661054547815	3.3117977926623694
5	0.7302067087137329	2.235411503281833
6	0.4310037131072009	1.5833390409696737
7	0.2958474197832218	1.2679656383744697
8	0.20202263968982426	0.9895362007429356
9	0.15498212446625317	0.8540155485579511
>10	0.7897669648088881	8.600986889502227
>50	0.05894227446820031	2.541441710747626
>100	0.04668819420102405	5.735227022761625
>500	0.00483881774954649	1.8839276096435038
>1k	9.154520066709576E-4	0.624479404437678
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1365	0.10837023081668282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5878422097682465E-4
2	0.0	0.0	0.0	0.0	1.5878422097682465E-4
3	0.0	0.0	0.0	0.0	1.5878422097682465E-4
4	0.0	0.0	0.0	0.0	1.5878422097682465E-4
5	0.0	0.0	0.0	0.0	1.5878422097682465E-4
6	0.0	0.0	0.0	0.0	1.5878422097682465E-4
7	0.0	0.0	0.0	0.0	1.5878422097682465E-4
8	0.0	0.0	0.0	0.0	1.5878422097682465E-4
9	0.0	0.0	0.0	0.0	1.5878422097682465E-4
10	0.0	0.0	0.0	0.0	1.5878422097682465E-4
11	0.0	0.0	0.0	0.0	2.3817633146523698E-4
12	0.0	0.0	0.0	0.0	3.175684419536493E-4
13	0.0	0.0	0.0	0.0	3.175684419536493E-4
14	0.0	0.0	0.0	0.0	3.175684419536493E-4
15	0.0	0.0	0.0	7.939211048841233E-5	3.175684419536493E-4
16	0.0	0.0	0.0	7.939211048841233E-5	3.175684419536493E-4
17	0.0	0.0	0.0	1.5878422097682465E-4	4.7635266293047395E-4
18	0.0	0.0	0.0	3.9696055244206163E-4	4.7635266293047395E-4
19	0.0	0.0	0.0	4.7635266293047395E-4	4.7635266293047395E-4
20	0.0	0.0	0.0	5.557447734188863E-4	4.7635266293047395E-4
21	0.0	0.0	0.0	7.939211048841233E-4	4.7635266293047395E-4
22	0.0	0.0	0.0	0.0013496658783030094	4.7635266293047395E-4
23	0.0	0.0	0.0	0.001508450099279834	4.7635266293047395E-4
24	0.0	0.0	0.0	0.0021435869831871327	4.7635266293047395E-4
25	0.0	0.0	0.0	0.002461155425140782	4.7635266293047395E-4
26	0.0	0.0	0.0	0.002937508088071256	4.7635266293047395E-4
27	0.0	0.0	0.0	0.0035726449719785548	4.7635266293047395E-4
28	0.0	0.0	0.0	0.004604742408327915	4.7635266293047395E-4
29	0.0	0.0	0.0	0.006113192507607749	4.7635266293047395E-4
30	0.0	7.939211048841233E-5	0.0	0.009368269037632654	4.7635266293047395E-4
31	0.0	1.5878422097682465E-4	0.0	0.01532267732426358	4.7635266293047395E-4
32	0.0	1.5878422097682465E-4	0.0	0.022706143599685924	4.7635266293047395E-4
33	0.0	1.5878422097682465E-4	0.0	0.029533865101689386	4.7635266293047395E-4
34	0.0	1.5878422097682465E-4	0.0	0.03890213413932204	4.7635266293047395E-4
35	0.0	1.5878422097682465E-4	0.0	0.04787344262451263	4.7635266293047395E-4
36	0.0	1.5878422097682465E-4	0.0	0.06057618030265861	4.7635266293047395E-4
37	0.0	1.5878422097682465E-4	0.0	0.08058299214573851	4.7635266293047395E-4
38	0.0	1.5878422097682465E-4	0.0	0.10773509393277553	4.7635266293047395E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	645	0.0	27.969898	1
GTATCAA	1470	0.0	26.939651	1
TCGTACG	50	0.0025794166	22.001587	41
CGTGCGT	70	8.123976E-4	18.857008	14
GTGCGTA	70	8.123976E-4	18.857008	15
TCAACGC	2175	0.0	17.902609	4
ATCAACG	2185	0.0	17.820673	3
GTAAACG	265	0.0	17.434528	27
CAACGCA	2245	0.0	17.344397	5
TATCAAC	2270	0.0	17.250977	2
GTCCTAT	315	0.0	16.762447	1
AACGCAG	2340	0.0	16.734257	6
TAAACGC	290	0.0	16.690197	28
GTACTAG	120	6.4049855E-6	16.500536	1
CGCAATA	240	0.0	16.49988	36
TAGGACG	80	0.0019890368	16.499226	4
TACACAA	270	0.0	16.295532	5
AACGCTT	285	0.0	16.21041	30
CCTATTC	300	0.0	16.132576	3
GTTACAC	195	8.931238E-10	15.79413	3
>>END_MODULE