FastQCFastQC Report
Thu 2 Feb 2017
SRR4062204_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062204_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15288
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGGGGTGAAATGGAATTT540.35321821036106754No Hit
CCTCTAGAGTATACATGAGAGAATT520.3401360544217687No Hit
GTACATGGGAGATCAAGCGGTGGTA500.3270538984824699No Hit
CTCTTACCCAATCCAGCAAAAGCAA390.25510204081632654No Hit
ATATAAGCCTCGTCCGACATGAAGG380.24856096284667714No Hit
GTGGTGGAGAACCTGGCACAGAACT380.24856096284667714No Hit
GTGGTACAGCAGGCTGCGCAGCAAG380.24856096284667714No Hit
GTACATGGGCAAGGCAATAATTATG370.24201988487702772No Hit
GTATCAACGCAGAGTACTTTTTTTT350.22893772893772896No Hit
CATATAGTCAGAGGCATTTCAACTG340.22239665096807953No Hit
GTGCATGGTCTCCATGGCCACGCGC340.22239665096807953No Hit
GTCCCAGCAGGCAGCGAGACGCATT330.2158555729984301No Hit
GTGTACCGGCATTGTGTGTTTTCAT320.20931449502878074No Hit
GGCATGTACCGGTCCAGCCAGCTCC310.20277341705913135No Hit
CTTCTACTGTTCGCAGTCATAGCCA300.19623233908948193No Hit
TATCAACGCAGAGTACTTTTTTTTT300.19623233908948193No Hit
GCCCAGTGCATGGTCTCCATGGCCA290.18969126111983253No Hit
GGCTAAACCTGGTGTAACTGGAACT290.18969126111983253No Hit
GATTATGAATGAACATATAGTCAGA280.18315018315018314No Hit
TGTTTGGACTGGTCATTAGGCTAGC280.18315018315018314No Hit
GGGCATGTACCGGTCCAGCCAGCTC270.17660910518053377No Hit
ATGTAGTAGTTTATTCTCAGTCGAC260.17006802721088435No Hit
AAAGTACACAGTGGTGGAGAACCTG260.17006802721088435No Hit
CCCTATACTCTGACCTTAAACACAC250.16352694924123495No Hit
GTACTGGAGCTGCGCAACGAGAGCG240.15698587127158556No Hit
AGTTTATTCTCAGTCGACCCATTCG240.15698587127158556No Hit
CACCTAGGCAGGGGGGACTAATGTC240.15698587127158556No Hit
CTCCATGGCCACGCGCTCGTGTGTA230.15044479330193616No Hit
GTCTAGGTCTGGTGAATAGCCTGGG230.15044479330193616No Hit
CCCCTATACTGGGCCCTATTCAGTG230.15044479330193616No Hit
CCTATCAGCCATCCCATATATTGGA220.14390371533228677No Hit
ACTCTCCACTGGGCGGGGGTCTTCG220.14390371533228677No Hit
GTATACATGAGAGAATTTAACGTTA220.14390371533228677No Hit
GTGTAAAAGGAGGGAAGCCTCTCTT220.14390371533228677No Hit
GTACCGGCATTGTGTGTTTTCATTC220.14390371533228677No Hit
GTACATGGGAGGCCAAGCCCAAGAA210.13736263736263737No Hit
AGCAAAGGGCGCCTTCGTGTTCCTC210.13736263736263737No Hit
AGGATACACAGAGAGCTACAGTCTC210.13736263736263737No Hit
CTCAAGGGCCGGGCTTTGGGCTCTT200.13082155939298795No Hit
GGGTAAGAGTCCTCCTCTAGCACCC200.13082155939298795No Hit
GGTATCAACGCAGAGTACTTTTTTT200.13082155939298795No Hit
GTCTCAAATAATGATTAAGTATGTC200.13082155939298795No Hit
GTTATATTCAGTCATATTCCCTGGA200.13082155939298795No Hit
GGCTTATATTATGGATCATATACAT190.12428048142333857No Hit
GTACATGCCCTGGCCATCCAGGCGA190.12428048142333857No Hit
GAGCAGGGAGGAGTCCACGAGACTT190.12428048142333857No Hit
TGCTGGGACTGCGTGGCCACCGCTC190.12428048142333857No Hit
GTTAGAAAGGAGTCTATGGCCAAGC190.12428048142333857No Hit
GTACAGCAGGCTGCGCAGCAAGTAC180.11773940345368916No Hit
GGTTAGTGGGAGGGGGTGCTAGAGG180.11773940345368916No Hit
ATTAAGAGGTCCCTCGAGTTTTCTT180.11773940345368916No Hit
CGCCATAGCAATGGAGATGTTACTG180.11773940345368916No Hit
GTCCACGAGACTTCACACGCTTGTG180.11773940345368916No Hit
GTTTTAAATAGCAATAAAGTACACA180.11773940345368916No Hit
GTACATGGGGAGTTTGTGCGTCTGT180.11773940345368916No Hit
AACATATAGTCAGAGGCATTTCAAC170.11119832548403977No Hit
GCATTGGTCTCACCTTACTTTTTTT170.11119832548403977No Hit
GATTAAATGTCCACATAACCTAATC170.11119832548403977No Hit
GTTTTACCTTGTCTTGTAATCAGGG170.11119832548403977No Hit
AACTAAGATGAAGAGAGGAACACGA170.11119832548403977No Hit
CTACACATCAGATACAATAACAGCC170.11119832548403977No Hit
GTACATGGGGGAATAGGGCAAGCCA170.11119832548403977No Hit
CTCGTGGACCAACCGGTGCCGTTGC160.10465724751439037No Hit
CTCTCATCCAGACACATTGAAACCC160.10465724751439037No Hit
CTAAAAACCCTCCTCTCAAGGAAGG160.10465724751439037No Hit
ATTCTCAGTCGACCCATTCGGATCG160.10465724751439037No Hit
GCCCTGAGCCCCGAGGTACTGGAGC160.10465724751439037No Hit
ACCATGATTGGTTCATGGAGCAGGG160.10465724751439037No Hit
TCACACGCTTGTGCTTTAACTTTCT160.10465724751439037No Hit
CTATAAATGTATATGATCCATAATA160.10465724751439037No Hit
GCTTTCACTGTGTACCGGCATTGTG160.10465724751439037No Hit
GTTTAGAAATCTCTCAGTACATGTT160.10465724751439037No Hit
GGGCTCATCCCCTCGAAACGAGAGC160.10465724751439037No Hit
GGTTATGAGAATTAAGAATATGATT160.10465724751439037No Hit
CACCACCGCCACGCGAAAATGCGTC160.10465724751439037No Hit
ACCCTAGTCGAATGAATTTGAGGGG160.10465724751439037No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)