##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062204_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15288
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.361067503924648	32.0	32.0	32.0	32.0	32.0
2	31.013867085295658	32.0	32.0	32.0	32.0	32.0
3	31.025248560962847	32.0	32.0	32.0	32.0	32.0
4	31.026949241234956	32.0	32.0	32.0	32.0	32.0
5	31.042255363683935	32.0	32.0	32.0	32.0	32.0
6	34.66163003663004	36.0	36.0	36.0	32.0	36.0
7	34.55952380952381	36.0	36.0	36.0	32.0	36.0
8	34.60374149659864	36.0	36.0	36.0	32.0	36.0
9	34.691915227629515	36.0	36.0	36.0	32.0	36.0
10	34.440345368916795	36.0	36.0	36.0	32.0	36.0
11	34.6342883307169	36.0	36.0	36.0	32.0	36.0
12	34.514063317634744	36.0	36.0	36.0	32.0	36.0
13	34.59929356357928	36.0	36.0	36.0	32.0	36.0
14	34.44289638932496	36.0	36.0	36.0	32.0	36.0
15	34.45499738356881	36.0	36.0	36.0	32.0	36.0
16	34.52008110936682	36.0	36.0	36.0	32.0	36.0
17	34.444858712715856	36.0	36.0	36.0	32.0	36.0
18	34.48914181057038	36.0	36.0	36.0	32.0	36.0
19	34.45264259549974	36.0	36.0	36.0	32.0	36.0
20	34.41875981161695	36.0	36.0	36.0	32.0	36.0
21	34.473574045002614	36.0	36.0	36.0	32.0	36.0
22	34.40953689167975	36.0	36.0	36.0	32.0	36.0
23	34.33810832025118	36.0	36.0	36.0	32.0	36.0
24	34.37114076399791	36.0	36.0	36.0	32.0	36.0
25	34.03721873364731	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	0.0
8	1.0
9	3.0
10	1.0
11	1.0
12	0.0
13	0.0
14	2.0
15	8.0
16	10.0
17	6.0
18	13.0
19	13.0
20	21.0
21	33.0
22	36.0
23	46.0
24	74.0
25	93.0
26	131.0
27	170.0
28	234.0
29	286.0
30	429.0
31	569.0
32	872.0
33	1229.0
34	3117.0
35	7886.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.61590790162219	19.538199895342753	12.029042386185242	24.816849816849818
2	17.667451596023025	20.65018315018315	38.52040816326531	23.16195709052852
3	18.66169544740973	25.549450549450547	29.34981684981685	26.439037153322868
4	14.488487702773417	17.248822605965465	35.485347985347985	32.77734170591313
5	13.144464799790631	38.39963360376865	35.01046846375294	13.445433132687779
6	31.108493652663267	36.36958513283602	18.623216856432403	13.898704358068315
7	29.128755645741965	31.4459645218302	22.046213261766052	17.37906657066178
8	25.955747577899974	34.275988478659336	21.059177795234355	18.709086148206335
9	27.125744583360607	16.488839431825618	20.468678405446095	35.91673757936768
10	16.021737707064755	28.13461664375041	32.14168794604858	23.70195770313625
11	35.029136384469325	25.679303345773587	21.60675702219603	17.684803247561057
12	24.11626080125687	25.792092170725322	28.803351662738937	21.28829536527887
13	29.90244221829372	20.552609179597983	26.844758724546587	22.700189877561712
14	23.02756498395862	20.035356511490864	24.782295554246055	32.15478295030446
15	22.857890947175495	28.768737317536164	23.807030176081692	24.56634155920665
16	24.569558101472996	28.903436988543373	24.44517184942717	22.081833060556466
17	21.603927986906708	29.505728314238954	27.00490998363339	21.88543371522095
18	22.057090480555193	28.185151237396884	27.49116145083148	22.266596831216447
19	24.397748101597276	26.970411102382823	25.471327572663	23.160513223356897
20	22.436820741128717	29.14757103574702	26.568024093230324	21.84758412989394
21	24.613773239067818	26.577638125163656	26.243781094527364	22.564807541241162
22	22.99031159989526	28.81644409531291	26.983503534956792	21.209740769835037
23	23.231000850952412	27.642861818419846	25.30601557897493	23.820121751652813
24	23.345335515548282	26.664484451718494	26.65139116202946	23.338788870703763
25	23.105118471004058	26.914517607016624	27.058515512501636	22.92184840947768
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	6.5
3	12.0
4	12.0
5	12.0
6	17.0
7	22.0
8	22.0
9	22.0
10	24.0
11	26.0
12	26.0
13	26.0
14	64.0
15	102.0
16	102.0
17	102.0
18	167.5
19	233.0
20	233.0
21	233.0
22	332.0
23	431.0
24	431.0
25	431.0
26	566.5
27	702.0
28	702.0
29	702.0
30	791.0
31	880.0
32	880.0
33	880.0
34	1122.0
35	1364.0
36	1364.0
37	1364.0
38	1422.0
39	1480.0
40	1480.0
41	1480.0
42	1697.0
43	1914.0
44	1914.0
45	1914.0
46	1964.0
47	2014.0
48	2014.0
49	2014.0
50	1808.5
51	1603.0
52	1603.0
53	1603.0
54	1447.5
55	1292.0
56	1292.0
57	1292.0
58	1158.0
59	1024.0
60	1024.0
61	1024.0
62	983.0
63	942.0
64	942.0
65	942.0
66	814.0
67	686.0
68	686.0
69	686.0
70	528.0
71	370.0
72	370.0
73	370.0
74	251.0
75	132.0
76	132.0
77	132.0
78	81.5
79	31.0
80	31.0
81	31.0
82	22.5
83	14.0
84	14.0
85	14.0
86	8.0
87	2.0
88	2.0
89	2.0
90	1.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	5.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.026164311878597593
6	0.03924646781789639
7	0.07195185766614338
8	0.07849293563579278
9	0.07195185766614338
10	0.09811616954474096
11	0.09811616954474096
12	0.07849293563579278
13	0.09811616954474096
14	0.09811616954474096
15	0.07195185766614338
16	0.08503401360544217
17	0.08503401360544217
18	0.09157509157509157
19	0.07849293563579278
20	0.09157509157509157
21	0.07849293563579278
22	0.07849293563579278
23	0.07195185766614338
24	0.08503401360544217
25	0.06541077969649398
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	15288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.715593929879645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.79224495636	30.991627420198846
2	13.263700100157392	12.127158555729984
3	5.5801974531406495	7.653061224489796
4	3.247961081699814	5.939298796441653
5	2.346544570038632	5.3636839351125065
6	1.6740592359421949	4.591836734693878
7	1.1875804836171127	3.8003663003663
8	1.0444984976391471	3.8199895342752486
9	0.7440263270854199	3.061224489795918
>10	3.0905708971240524	21.95839874411303
>50	0.028616397195593075	0.6933542647828362
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATAGTAGGGGTGAAATGGAATTT	54	0.35321821036106754	No Hit
CCTCTAGAGTATACATGAGAGAATT	52	0.3401360544217687	No Hit
GTACATGGGAGATCAAGCGGTGGTA	50	0.3270538984824699	No Hit
CTCTTACCCAATCCAGCAAAAGCAA	39	0.25510204081632654	No Hit
ATATAAGCCTCGTCCGACATGAAGG	38	0.24856096284667714	No Hit
GTGGTGGAGAACCTGGCACAGAACT	38	0.24856096284667714	No Hit
GTGGTACAGCAGGCTGCGCAGCAAG	38	0.24856096284667714	No Hit
GTACATGGGCAAGGCAATAATTATG	37	0.24201988487702772	No Hit
GTATCAACGCAGAGTACTTTTTTTT	35	0.22893772893772896	No Hit
CATATAGTCAGAGGCATTTCAACTG	34	0.22239665096807953	No Hit
GTGCATGGTCTCCATGGCCACGCGC	34	0.22239665096807953	No Hit
GTCCCAGCAGGCAGCGAGACGCATT	33	0.2158555729984301	No Hit
GTGTACCGGCATTGTGTGTTTTCAT	32	0.20931449502878074	No Hit
GGCATGTACCGGTCCAGCCAGCTCC	31	0.20277341705913135	No Hit
CTTCTACTGTTCGCAGTCATAGCCA	30	0.19623233908948193	No Hit
TATCAACGCAGAGTACTTTTTTTTT	30	0.19623233908948193	No Hit
GCCCAGTGCATGGTCTCCATGGCCA	29	0.18969126111983253	No Hit
GGCTAAACCTGGTGTAACTGGAACT	29	0.18969126111983253	No Hit
GATTATGAATGAACATATAGTCAGA	28	0.18315018315018314	No Hit
TGTTTGGACTGGTCATTAGGCTAGC	28	0.18315018315018314	No Hit
GGGCATGTACCGGTCCAGCCAGCTC	27	0.17660910518053377	No Hit
ATGTAGTAGTTTATTCTCAGTCGAC	26	0.17006802721088435	No Hit
AAAGTACACAGTGGTGGAGAACCTG	26	0.17006802721088435	No Hit
CCCTATACTCTGACCTTAAACACAC	25	0.16352694924123495	No Hit
GTACTGGAGCTGCGCAACGAGAGCG	24	0.15698587127158556	No Hit
AGTTTATTCTCAGTCGACCCATTCG	24	0.15698587127158556	No Hit
CACCTAGGCAGGGGGGACTAATGTC	24	0.15698587127158556	No Hit
CTCCATGGCCACGCGCTCGTGTGTA	23	0.15044479330193616	No Hit
GTCTAGGTCTGGTGAATAGCCTGGG	23	0.15044479330193616	No Hit
CCCCTATACTGGGCCCTATTCAGTG	23	0.15044479330193616	No Hit
CCTATCAGCCATCCCATATATTGGA	22	0.14390371533228677	No Hit
ACTCTCCACTGGGCGGGGGTCTTCG	22	0.14390371533228677	No Hit
GTATACATGAGAGAATTTAACGTTA	22	0.14390371533228677	No Hit
GTGTAAAAGGAGGGAAGCCTCTCTT	22	0.14390371533228677	No Hit
GTACCGGCATTGTGTGTTTTCATTC	22	0.14390371533228677	No Hit
GTACATGGGAGGCCAAGCCCAAGAA	21	0.13736263736263737	No Hit
AGCAAAGGGCGCCTTCGTGTTCCTC	21	0.13736263736263737	No Hit
AGGATACACAGAGAGCTACAGTCTC	21	0.13736263736263737	No Hit
CTCAAGGGCCGGGCTTTGGGCTCTT	20	0.13082155939298795	No Hit
GGGTAAGAGTCCTCCTCTAGCACCC	20	0.13082155939298795	No Hit
GGTATCAACGCAGAGTACTTTTTTT	20	0.13082155939298795	No Hit
GTCTCAAATAATGATTAAGTATGTC	20	0.13082155939298795	No Hit
GTTATATTCAGTCATATTCCCTGGA	20	0.13082155939298795	No Hit
GGCTTATATTATGGATCATATACAT	19	0.12428048142333857	No Hit
GTACATGCCCTGGCCATCCAGGCGA	19	0.12428048142333857	No Hit
GAGCAGGGAGGAGTCCACGAGACTT	19	0.12428048142333857	No Hit
TGCTGGGACTGCGTGGCCACCGCTC	19	0.12428048142333857	No Hit
GTTAGAAAGGAGTCTATGGCCAAGC	19	0.12428048142333857	No Hit
GTACAGCAGGCTGCGCAGCAAGTAC	18	0.11773940345368916	No Hit
GGTTAGTGGGAGGGGGTGCTAGAGG	18	0.11773940345368916	No Hit
ATTAAGAGGTCCCTCGAGTTTTCTT	18	0.11773940345368916	No Hit
CGCCATAGCAATGGAGATGTTACTG	18	0.11773940345368916	No Hit
GTCCACGAGACTTCACACGCTTGTG	18	0.11773940345368916	No Hit
GTTTTAAATAGCAATAAAGTACACA	18	0.11773940345368916	No Hit
GTACATGGGGAGTTTGTGCGTCTGT	18	0.11773940345368916	No Hit
AACATATAGTCAGAGGCATTTCAAC	17	0.11119832548403977	No Hit
GCATTGGTCTCACCTTACTTTTTTT	17	0.11119832548403977	No Hit
GATTAAATGTCCACATAACCTAATC	17	0.11119832548403977	No Hit
GTTTTACCTTGTCTTGTAATCAGGG	17	0.11119832548403977	No Hit
AACTAAGATGAAGAGAGGAACACGA	17	0.11119832548403977	No Hit
CTACACATCAGATACAATAACAGCC	17	0.11119832548403977	No Hit
GTACATGGGGGAATAGGGCAAGCCA	17	0.11119832548403977	No Hit
CTCGTGGACCAACCGGTGCCGTTGC	16	0.10465724751439037	No Hit
CTCTCATCCAGACACATTGAAACCC	16	0.10465724751439037	No Hit
CTAAAAACCCTCCTCTCAAGGAAGG	16	0.10465724751439037	No Hit
ATTCTCAGTCGACCCATTCGGATCG	16	0.10465724751439037	No Hit
GCCCTGAGCCCCGAGGTACTGGAGC	16	0.10465724751439037	No Hit
ACCATGATTGGTTCATGGAGCAGGG	16	0.10465724751439037	No Hit
TCACACGCTTGTGCTTTAACTTTCT	16	0.10465724751439037	No Hit
CTATAAATGTATATGATCCATAATA	16	0.10465724751439037	No Hit
GCTTTCACTGTGTACCGGCATTGTG	16	0.10465724751439037	No Hit
GTTTAGAAATCTCTCAGTACATGTT	16	0.10465724751439037	No Hit
GGGCTCATCCCCTCGAAACGAGAGC	16	0.10465724751439037	No Hit
GGTTATGAGAATTAAGAATATGATT	16	0.10465724751439037	No Hit
CACCACCGCCACGCGAAAATGCGTC	16	0.10465724751439037	No Hit
ACCCTAGTCGAATGAATTTGAGGGG	16	0.10465724751439037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.006541077969649398	0.0
5	0.0	0.0	0.0	0.006541077969649398	0.0
6	0.0	0.0	0.0	0.006541077969649398	0.0
7	0.0	0.0	0.0	0.006541077969649398	0.0
8	0.0	0.0	0.0	0.006541077969649398	0.0
9	0.0	0.0	0.0	0.006541077969649398	0.0
10	0.0	0.0	0.0	0.006541077969649398	0.0
11	0.0	0.0	0.0	0.006541077969649398	0.0
12	0.0	0.0	0.0	0.006541077969649398	0.0
13	0.0	0.0	0.0	0.006541077969649398	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE