FastQCFastQC Report
Thu 2 Feb 2017
SRR4062204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15288
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT490.3205128205128205No Hit
CCTCTAGAGTATACATGAGAGAATTTAACGTTAACATCTTAAGAACTGGT490.3205128205128205No Hit
CATATAGTCAGAGGCATTTCAACTGAAACAAACTCATTTGATTAAATGTC490.3205128205128205No Hit
GTCCCAGCAGGCAGCGAGACGCATTTTCGCGTGGCGGTGGTGAGCTCTCG470.3074306645735217No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG440.28780743066457354No Hit
GTACATGGGAGATCAAGCGGTGGTACAGCAGGCTGCGCAGCAAGTACAAA430.2812663526949241No Hit
GGCATGTACCGGTCCAGCCAGCTCCTCCGACAGTGCCTCGTGGACCAACC360.23547880690737832No Hit
CTCTTACCCAATCCAGCAAAAGCAAACAGACTTGCAACTGGACCTCGGCC360.23547880690737832No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.22239665096807953No Hit
GTGCATGGTCTCCATGGCCACGCGCTCGTGTGTATCGCGGGGCAGCGCGG330.2158555729984301No Hit
CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC330.2158555729984301No Hit
GTACTGGAGCTGCGCAACGAGAGCGGTGGCCACGCAGTCCCAGCAGGCAG320.20931449502878074No Hit
AGTATGTCGTGCTTAAAAACAAGAAACCGTAAAAATGTCACCTCTCGGCT310.20277341705913135No Hit
TAATTATGTTGCATCTGTGAACTTTGGCAGGTTTGTGTAAAAGGAGGGAA280.18315018315018314No Hit
GGGCATGTACCGGTCCAGCCAGCTCCTCCGACAGTGCCTCGTGGACCAAC280.18315018315018314No Hit
GTGTACCGGCATTGTGTGTTTTCATTCTCGCCATAGCAATGGAGATGTTA260.17006802721088435No Hit
GTGGTACAGCAGGCTGCGCAGCAAGTACAAAGCGAATGCTAAGTGCGCGA260.17006802721088435No Hit
GTCTAGGTCTGGTGAATAGCCTGGGGAAAGGGCAACCTTTCAGCAGTATC250.16352694924123495No Hit
GTACATGCCCTGGCCATCCAGGCGAAGACCCCCGCCCAGTGGAGAGTAAA250.16352694924123495No Hit
CCCTATACTCTGACCTTAAACACACACACTCTCATCCAGACACATTGAAA250.16352694924123495No Hit
CTCTTAAAACAGCAATAACAGAAAAGGAGGAAGCGGGATGTTTTACCTTG230.15044479330193616No Hit
AGTTTATTCTCAGTCGACCCATTCGGATCGTTCTCCCAATCCAGGTATTT230.15044479330193616No Hit
CACCTAGGCAGGGGGGACTAATGTCCAACTGTGGGTTTACTCTCCACTGG230.15044479330193616No Hit
GTGGTGGAGAACCTGGCACAGAACTTACAAGCATCTCAGTCACAAGAAGG230.15044479330193616No Hit
GCCCAGTGCATGGTCTCCATGGCCACGCGCTCGTGTGTATCGCGGGGCAG230.15044479330193616No Hit
CATTTATAGAAACCTGAAACATTGGAGTACTTCTACTGTTCGCAGTCATA220.14390371533228677No Hit
ATGTAGTAGTTTATTCTCAGTCGACCCATTCGGATCGTTCTCCCAATCCA220.14390371533228677No Hit
GGGTAAGAGTCCTCCTCTAGCACCCCCTCCCACTAACCACTGCTTTTCCC220.14390371533228677No Hit
GAATATAACATATCTTTGTAATTGAATTAGTTTTTGCCACTTCTAACTGA220.14390371533228677No Hit
CTTAAGAACTGGTAACAGTTCGTGTTACTAAGCTTTCACTGTGTACCGGC210.13736263736263737No Hit
ACCTAGGATATCTTTGATTGTATAGTAGGGGTGAAATGGAATTTTATCTG210.13736263736263737No Hit
GTCATAGCCACAGCATTTATAGGCTACGTCCTTCCATGAGGACAAATATC210.13736263736263737No Hit
GTCTAACACAAGTATTTATTTTTTCAGTTAAACATGTATTTCATTCTAAA210.13736263736263737No Hit
ACCCTAGTCGAATGAATTTGAGGGGGCTTCTCAGTAGACAAAGCCACCTT210.13736263736263737No Hit
GATTATGAATGAACATATAGTCAGAGGCATTTCAACTGAAACAAACTCAT200.13082155939298795No Hit
GGCTAAACCTGGTGTAACTGGAACTTAGCTGATTTCACTCACGTGGGAAA200.13082155939298795No Hit
TGCTGGGACTGCGTGGCCACCGCTCTCGTTGCGCAGCTCCAGTACCTCGG200.13082155939298795No Hit
GTACCGGCATTGTGTGTTTTCATTCTCGCCATAGCAATGGAGATGTTACT200.13082155939298795No Hit
TTCATTGACCTACCTGCCCCATCCAACATTTCATCATGATGAAACTTTGG190.12428048142333857No Hit
CTACACATCAGATACAATAACAGCCTTTTCATCAGTAACACACATTTGTC190.12428048142333857No Hit
GTTATATTCAGTCATATTCCCTGGATGCAGACAACTGTGAGGATACAGCA190.12428048142333857No Hit
ATTAAGAGGTCCCTCGAGTTTTCTTGCTCCCACCTAGGCAGGGGGGACTA190.12428048142333857No Hit
TGTTTGGACTGGTCATTAGGCTAGCCTAATCTCTGCATAGCTTGCAGCTG190.12428048142333857No Hit
CCTATCAGCCATCCCATATATTGGAACAACCCTAGTCGAATGAATTTGAG180.11773940345368916No Hit
CCCATATATTGGAACAACCCTAGTCGAATGAATTTGAGGGGGCTTCTCAG180.11773940345368916No Hit
GTCATATTCCCTGGATGCAGACAACTGTGAGGATACAGCAACTTACCTTT180.11773940345368916No Hit
ATCTTAGTTAATGCGCGAGGAAAATGCTTTGATGGCCATTTCTCATTCGC180.11773940345368916No Hit
GTACAAAGCGAATGCTAAGTGCGCGATCGGCCCAGTGCATGGTCTCCATG180.11773940345368916No Hit
GTGTAAAAGGAGGGAAGCCTCTCTTAAAACAGCAATAACAGAAAAGGAGG180.11773940345368916No Hit
GTATACATGAGAGAATTTAACGTTAACATCTTAAGAACTGGTAACAGTTC180.11773940345368916No Hit
GCCATACACTACACATCAGATACAATAACAGCCTTTTCATCAGTAACACA170.11119832548403977No Hit
CTTCCAGACGAGCCACTTCTGGCAGAGACAACTAACTTCTCTGTGCTTCA170.11119832548403977No Hit
GTACATGGGGGGACCTCTAGAGTATACATGAGAGAATTTAACGTTAACAT170.11119832548403977No Hit
GTTTTAAATAGCAATAAAGTACACAGTGGTGGAGAACCTGGCACAGAACT170.11119832548403977No Hit
ACCATGATTGGTTCATGGAGCAGGGAGGAGTCCACGAGACTTCACACGCT170.11119832548403977No Hit
GTCTCAAATAATGATTAAGTATGTCGTGCTTAAAAACAAGAAACCGTAAA170.11119832548403977No Hit
ACTCTCCACTGGGCGGGGGTCTTCGCCTGGATGGCCAGGGCATGTACCGG170.11119832548403977No Hit
GTACATGTTTTCTTGCCCCATGGCATATCCCCAAATTTAGCTAAAATAAT170.11119832548403977No Hit
AGGATACACAGAGAGCTACAGTCTCCAACCAAAACCAGAACAGAAATCCA170.11119832548403977No Hit
GCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCCATACACTACACA170.11119832548403977No Hit
CTTGTGCTTTAACTTTCTTAACTGGGCACAAGCAAAGGGCGCCTTCGTGT160.10465724751439037No Hit
GTTCACAGCCATGTTGGTGGGCACAAAAGATGTCTCGCTATCCTTCTTCT160.10465724751439037No Hit
AACATATAGTCAGAGGCATTTCAACTGAAACAAACTCATTTGATTAAATG160.10465724751439037No Hit
TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCC160.10465724751439037No Hit
CTTGTATGTCTCTCCAGTGTGCTTATATAAAAAGAAGTGTTCTGCCTTTA160.10465724751439037No Hit
CGTCAGAGAGGAGGCAGCATTGGTCTCACCTTACTTTTTTTACATTACCA160.10465724751439037No Hit
GTACATGGGCAAGGCAATAATTATGTTGCATCTGTGAACTTTGGCAGGTT160.10465724751439037No Hit
GTACATGGGAGGCCAAGCCCAAGAATGCTGAGGACTGCCTCTATGAGCTG160.10465724751439037No Hit
GCTTTCACTGTGTACCGGCATTGTGTGTTTTCATTCTCGCCATAGCAATG160.10465724751439037No Hit
CCAGAGGACAACGATTTAGAAAAATAGCTTGCATTTTTGTTATAGGAAAG160.10465724751439037No Hit
GTCCACGAGACTTCACACGCTTGTGCTTTAACTTTCTTAACTGGGCACAA160.10465724751439037No Hit
TTTATAGGCTACGTCCTTCCATGAGGACAAATATCATTCTGAGGTGCCAC160.10465724751439037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA301.2418741E-436.6666641
TCAACGC450.001336001224.4444454
TATCAAC450.001336001224.4444452
ATCAACG450.001336001224.4444453
AACGCAG500.002466520322.06
CAACGCA500.002466520322.05
ACGCAGA550.004287228520.07
AGAGTAC550.004287228520.011
CGCAGAG550.004287228520.08
Produced by FastQC (version 0.11.5)