FastQCFastQC Report
Thu 2 Feb 2017
SRR4062203_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062203_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269012
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC18320.14436427709115437No Hit
GTATCAACGCAGAGTACTTTTTTTT17990.14176382886844255No Hit
GGGTAGGCACACGCTGAGCCAGTCA17410.13719334411337322No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC14010.11040084727331184No Hit
GAATAGGACCGCGGTTCTATTTTGT13840.10906122243130875No Hit
GATTAAGAGGGACGGCCGGGGGCAT13600.10716998736024562No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13280.10464834059882808No Hit
TCGTAGTTCCGACCATAAACGATGC13010.10252070114388201No Hit
ATCAGATACCGTCGTAGTTCCGACC12940.10196909091482194No Hit
GTATCTGATCGTCTTCGAACCTCCG12770.10062946607281885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5550.013.85979751
GGTATAG500.001503416113.2951391
CGGACCA3100.013.1798849
CGGACAT1550.012.8728665
CGACCCG1700.012.8548375
ACGGTAT2250.012.6690359
GTCTTAC751.483638E-512.6620381
GTTCAAA3250.012.5646371
AAGACGG3300.012.3806145
CGGTCCA2150.012.37391910
CTAGACC701.08949476E-412.2156054
CGGTAAT701.0943217E-412.2098221
CGCATCG1800.012.13780413
TTAACGA1103.8073267E-812.09173912
GAATTTC2200.012.08935218
GTATAAT550.003076317312.0864911
GTATCAA13600.012.0801431
GACCCGG2050.012.051516
CAAGACG3550.012.0435544
TTTCGTC1501.4551915E-1112.03130917
Produced by FastQC (version 0.11.5)