##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062203_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1269012
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254055123198203	32.0	32.0	32.0	32.0	32.0
2	30.744080434227573	32.0	32.0	32.0	32.0	32.0
3	30.775026556092456	32.0	32.0	32.0	32.0	32.0
4	30.783271552987678	32.0	32.0	32.0	32.0	32.0
5	30.670897517123556	32.0	32.0	32.0	32.0	32.0
6	34.34587064582526	36.0	36.0	36.0	32.0	36.0
7	34.28977030950062	36.0	36.0	36.0	32.0	36.0
8	34.23196470955357	36.0	36.0	36.0	32.0	36.0
9	34.395833924344295	36.0	36.0	36.0	32.0	36.0
10	34.031678187440306	36.0	36.0	36.0	32.0	36.0
11	34.368684456884566	36.0	36.0	36.0	32.0	36.0
12	34.16890620419665	36.0	36.0	36.0	32.0	36.0
13	34.267973037292	36.0	36.0	36.0	32.0	36.0
14	34.15816083693456	36.0	36.0	36.0	32.0	36.0
15	34.09688482063211	36.0	36.0	36.0	32.0	36.0
16	34.11379876628432	36.0	36.0	36.0	32.0	36.0
17	34.028742833007094	36.0	36.0	36.0	32.0	36.0
18	34.03962373878261	36.0	36.0	36.0	32.0	36.0
19	34.04063870160408	36.0	36.0	36.0	32.0	36.0
20	34.04372771888682	36.0	36.0	36.0	32.0	36.0
21	34.02402262547557	36.0	36.0	36.0	32.0	36.0
22	33.99099772106174	36.0	36.0	36.0	32.0	36.0
23	33.944068298802534	36.0	36.0	36.0	32.0	36.0
24	33.92380607905993	36.0	36.0	36.0	32.0	36.0
25	33.55850850898179	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	19.0
5	101.0
6	228.0
7	64.0
8	220.0
9	150.0
10	90.0
11	34.0
12	51.0
13	50.0
14	182.0
15	271.0
16	404.0
17	582.0
18	828.0
19	1137.0
20	1707.0
21	2515.0
22	3790.0
23	5711.0
24	8203.0
25	11440.0
26	15540.0
27	19755.0
28	26083.0
29	34481.0
30	45258.0
31	61311.0
32	86949.0
33	123442.0
34	265438.0
35	552977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.82199240032719	17.007774613436386	11.234418858283911	25.93581412795252
2	17.501688325299504	18.806170799419075	36.61487034370914	27.077270531572275
3	19.962710972209745	21.846153603686666	27.501052004482247	30.690083419621338
4	13.366307372582211	14.967881047571987	33.731874720717606	37.933936859128195
5	15.685733541961447	35.662558479589784	32.532821485017	16.11888649343176
6	36.57859046537667	33.51256091214458	15.714623645739698	14.19422497673905
7	31.946638983266112	29.249085130400964	19.233363335954948	19.57091255037798
8	28.914535443257655	32.711847025227584	18.227284918669042	20.146332612845715
9	26.776465427670015	14.599485140797345	18.25880392668897	40.365245504843664
10	17.25667592217796	26.14386047139195	29.69109632438634	26.908367282043756
11	38.16195972194713	20.97927702781081	20.851440254852864	20.007322995389195
12	24.94046595157424	23.192003421333784	27.52761290953823	24.339917717553746
13	29.76428112686813	18.790997931266283	24.609883529187133	26.834837412678446
14	23.843138987415273	19.19297426252477	24.02174873458465	32.9421380154753
15	25.657678784473887	26.80575632665686	21.250796947297324	26.285767941571926
16	26.658135793927357	25.395151129775247	22.60213182407363	25.344581252223776
17	24.790826587055193	25.903420069128806	24.037841748527995	25.267911595288005
18	25.55889951681294	24.68474509417217	25.03114091312494	24.72521447588995
19	26.09552294371065	24.698976686369438	24.32495531777619	24.88054505214372
20	26.173838150252543	24.08288718597483	23.978461887921053	25.76481277585157
21	26.946674522219144	24.040041233763418	23.881116150968577	25.13216809304886
22	26.61279254123382	23.669829700970997	24.391096176751304	25.32628158104388
23	24.903826893139417	23.871829126532106	25.091993381839174	26.132350598489307
24	25.206246549412413	24.50477166969004	24.683334647842887	25.60564713305466
25	25.33684665472074	24.179059510890138	24.510464250561544	25.973629583827574
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	193.0
1	193.0
2	250.0
3	307.0
4	307.0
5	307.0
6	684.0
7	1061.0
8	1061.0
9	1061.0
10	1300.5
11	1540.0
12	1540.0
13	1540.0
14	2023.0
15	2506.0
16	2506.0
17	2506.0
18	4168.0
19	5830.0
20	5830.0
21	5830.0
22	9519.0
23	13208.0
24	13208.0
25	13208.0
26	20221.0
27	27234.0
28	27234.0
29	27234.0
30	34664.5
31	42095.0
32	42095.0
33	42095.0
34	54095.0
35	66095.0
36	66095.0
37	66095.0
38	79695.5
39	93296.0
40	93296.0
41	93296.0
42	109338.5
43	125381.0
44	125381.0
45	125381.0
46	142425.5
47	159470.0
48	159470.0
49	159470.0
50	168221.0
51	176972.0
52	176972.0
53	176972.0
54	169015.5
55	161059.0
56	161059.0
57	161059.0
58	151043.0
59	141027.0
60	141027.0
61	141027.0
62	125109.5
63	109192.0
64	109192.0
65	109192.0
66	89410.0
67	69628.0
68	69628.0
69	69628.0
70	52708.5
71	35789.0
72	35789.0
73	35789.0
74	27100.5
75	18412.0
76	18412.0
77	18412.0
78	14807.0
79	11202.0
80	11202.0
81	11202.0
82	7675.0
83	4148.0
84	4148.0
85	4148.0
86	3068.5
87	1989.0
88	1989.0
89	1989.0
90	1337.0
91	685.0
92	685.0
93	685.0
94	425.5
95	166.0
96	166.0
97	166.0
98	346.5
99	527.0
100	527.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.880146129429825E-4
2	2.3640438388289472E-4
3	4.7280876776578944E-4
4	0.010638197274730263
5	0.03207219474677939
6	0.06241075734508421
7	0.10598796544083113
8	0.10653957566989124
9	0.1167049641768557
10	0.13073162428724078
11	0.13955738795220218
12	0.13159844036147805
13	0.12371829423204823
14	0.11378931010896666
15	0.13207124912924384
16	0.11497133202838113
17	0.12001462555121623
18	0.10929762681519166
19	0.09219770971432895
20	0.08825763664961403
21	0.08817883518831973
22	0.09787141492751841
23	0.07769824083617806
24	0.08762722495925965
25	0.08479037235266491
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1269012.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.22727681834829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71292815027574	40.8322026220079
2	13.594740680459058	14.472220509449096
3	4.198261841513533	6.703861355824486
4	1.819177003684953	3.873193518268496
5	0.952524074819771	2.535013130328652
6	0.5946035274181849	1.8989475930632445
7	0.3922210036726176	1.461379915551697
8	0.2820403883062672	1.20097934578657
9	0.22489069302804543	1.0773287254506567
>10	1.0642340594663124	10.19372214258369
>50	0.0805930280400148	2.9956932415338624
>100	0.07336817540408203	8.023770361836966
>500	0.007738620619992307	2.8343865051055817
>1k	0.002678753291535799	1.8973010332091242
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	1832	0.14436427709115437	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1799	0.14176382886844255	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1741	0.13719334411337322	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1401	0.11040084727331184	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1384	0.10906122243130875	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1360	0.10716998736024562	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1328	0.10464834059882808	No Hit
TCGTAGTTCCGACCATAAACGATGC	1301	0.10252070114388201	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1294	0.10196909091482194	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1277	0.10062946607281885	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3640438388289472E-4
2	0.0	0.0	0.0	0.0	2.3640438388289472E-4
3	0.0	0.0	0.0	0.0	2.3640438388289472E-4
4	0.0	0.0	0.0	0.0	2.3640438388289472E-4
5	0.0	0.0	0.0	0.0	2.3640438388289472E-4
6	0.0	0.0	0.0	0.0	3.15205845177193E-4
7	0.0	0.0	0.0	0.0	3.15205845177193E-4
8	0.0	0.0	0.0	0.0	3.940073064714912E-4
9	0.0	0.0	0.0	0.0	3.940073064714912E-4
10	0.0	0.0	0.0	0.0	3.940073064714912E-4
11	0.0	0.0	0.0	0.0	3.940073064714912E-4
12	0.0	0.0	0.0	7.880146129429824E-5	0.0011820219194144737
13	0.0	0.0	0.0	1.576029225885965E-4	0.0014972277645916665
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	555	0.0	13.8597975	1
GGTATAG	50	0.0015034161	13.295139	1
CGGACCA	310	0.0	13.179884	9
CGGACAT	155	0.0	12.872866	5
CGACCCG	170	0.0	12.854837	5
ACGGTAT	225	0.0	12.669035	9
GTCTTAC	75	1.483638E-5	12.662038	1
GTTCAAA	325	0.0	12.564637	1
AAGACGG	330	0.0	12.380614	5
CGGTCCA	215	0.0	12.373919	10
CTAGACC	70	1.08949476E-4	12.215605	4
CGGTAAT	70	1.0943217E-4	12.209822	1
CGCATCG	180	0.0	12.137804	13
TTAACGA	110	3.8073267E-8	12.091739	12
GAATTTC	220	0.0	12.089352	18
GTATAAT	55	0.0030763173	12.086491	1
GTATCAA	1360	0.0	12.080143	1
GACCCGG	205	0.0	12.05151	6
CAAGACG	355	0.0	12.043554	4
TTTCGTC	150	1.4551915E-11	12.031309	17
>>END_MODULE