FastQCFastQC Report
Thu 2 Feb 2017
SRR4062203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269012
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18240.1437338654008No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15320.12072383870286492No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14680.11568054518002982No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14400.11347410426378945No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13960.11000683996684035No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13850.10914002389260306No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13660.10764279612801139No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13590.10709118589895131No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13090.1031511128342364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC655.9359627E-727.0746443
GGTATCA6450.025.9315381
GTATCAA13550.025.3372631
TAACGGC551.5928858E-424.0017636
TTAACGG654.932528E-420.30758335
TCCCGTA654.93515E-420.30598313
CGAGCCG4550.018.85555515
GTCCTAC2400.018.3397371
TAGGACG1102.7988972E-617.9984844
AATTCCG3700.017.84055342
CTATACT1002.4849673E-517.5985184
GTTCAAA5900.017.5315481
TTCCGAT4100.017.17334644
AGCGAAA6050.017.0894717
ACGGACC5950.017.0069718
GCGAAAG6100.016.94939218
AGGCCCG5200.016.92165210
CGAAAGC6400.016.84233319
GTCCTAT3550.016.7382411
GACGGAC6050.016.7258647
Produced by FastQC (version 0.11.5)