FastQCFastQC Report
Thu 2 Feb 2017
SRR4062202_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062202_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449269
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10370.23081939773276142No Hit
TATCAACGCAGAGTACTTTTTTTTT6580.14646013858067217No Hit
GCGCAAGACGGACCAGAGCGAAAGC4940.10995639583412165No Hit
GAATAGGACCGCGGTTCTATTTTGT4550.10127562774195416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACT250.006018185519.0040155
GCGTTAT350.002173599616.2782651
CAAGACG651.9022264E-716.0785274
CGGTCCA551.1236783E-515.54700610
GCAAGAC1058.731149E-1114.4760213
GTTATTC400.005271769614.2498323
CGGACCA707.227578E-613.57278259
AAGACTC500.001494266813.3028115
CCTAGAG500.001496780713.2998452
GGTTCTA655.430553E-513.15515813
CTTGCGC801.9906365E-613.0623463
GACGGAC751.4698413E-512.6693437
CGAAGAC604.078512E-412.6679314
CGTCGTA751.4715282E-512.6679310
GTAGAAC604.0823512E-412.6665193
CCTATTC751.4732166E-512.6665183
CCCTAGA604.0977393E-412.6608721
GTCCTAT905.4062184E-712.6608721
CGCAAGA701.0876868E-412.2141422
CTACACC550.003060309212.0921154
Produced by FastQC (version 0.11.5)