##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062202_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449269
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22567548617866	32.0	32.0	32.0	32.0	32.0
2	30.739338792571935	32.0	32.0	32.0	32.0	32.0
3	30.779386069370467	32.0	32.0	32.0	32.0	32.0
4	30.787686219169363	32.0	32.0	32.0	32.0	32.0
5	30.69778684930409	32.0	32.0	32.0	32.0	32.0
6	34.36052120221961	36.0	36.0	36.0	32.0	36.0
7	34.28010835379249	36.0	36.0	36.0	32.0	36.0
8	34.248330065061246	36.0	36.0	36.0	32.0	36.0
9	34.3713410006032	36.0	36.0	36.0	32.0	36.0
10	34.048986242095495	36.0	36.0	36.0	32.0	36.0
11	34.365774179834354	36.0	36.0	36.0	32.0	36.0
12	34.15113884999855	36.0	36.0	36.0	32.0	36.0
13	34.2722021773147	36.0	36.0	36.0	32.0	36.0
14	34.1662856774004	36.0	36.0	36.0	32.0	36.0
15	34.119500789059565	36.0	36.0	36.0	32.0	36.0
16	34.10893696204278	36.0	36.0	36.0	32.0	36.0
17	34.05652292946987	36.0	36.0	36.0	32.0	36.0
18	34.08506930146527	36.0	36.0	36.0	32.0	36.0
19	34.055113974033375	36.0	36.0	36.0	32.0	36.0
20	34.031373186220286	36.0	36.0	36.0	32.0	36.0
21	34.00409331603115	36.0	36.0	36.0	32.0	36.0
22	33.9821688120035	36.0	36.0	36.0	32.0	36.0
23	33.940734838148195	36.0	36.0	36.0	32.0	36.0
24	33.92103617209289	36.0	36.0	36.0	32.0	36.0
25	33.56280980882278	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	7.0
5	33.0
6	98.0
7	21.0
8	64.0
9	65.0
10	21.0
11	9.0
12	23.0
13	31.0
14	105.0
15	110.0
16	160.0
17	265.0
18	374.0
19	454.0
20	661.0
21	945.0
22	1489.0
23	2074.0
24	2915.0
25	3966.0
26	5553.0
27	7104.0
28	9303.0
29	11892.0
30	15776.0
31	21059.0
32	29851.0
33	42395.0
34	92345.0
35	200100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.23234513106905	17.45131514222055	11.510527147553681	25.80581257915672
2	16.397339672533988	19.60789551002965	38.11288584987135	25.88187896756502
3	19.59195665819359	23.097007118277368	27.94157581477343	29.369460408755614
4	12.828617857222358	15.42795449992209	35.4270640874385	36.31636355541705
5	14.789077355373372	36.65507367708551	33.21680968645491	15.339039281086208
6	34.505798479722586	35.190435570839	16.608351503462163	13.695414445976253
7	30.555450338341426	30.173326173611375	20.062744119409903	19.2084793686373
8	27.817489850396406	33.78269375604408	19.171498219641432	19.228318173918083
9	27.236425462883528	14.721356261545626	18.681352963591028	39.36086531197982
10	16.74701077569396	26.67312985435541	30.99477373271376	25.585085637236876
11	36.84677415759888	21.302640318460835	22.33861872517597	19.511966798764316
12	24.733440313809396	23.774404921101898	28.660292413301242	22.831862351787464
13	29.518828125522322	19.717327402349365	25.093990198722132	25.66985427340618
14	23.213676889719824	20.26999021742279	25.02668482051533	31.489648072342046
15	24.872796646244296	28.059399453075724	21.89234907765032	25.175454823029657
16	25.285127889719348	26.015607938566582	24.270299902394672	24.42896426931939
17	23.493796017328368	26.431328329738115	25.47554953380992	24.5993261191236
18	24.17149261679765	25.308329749615076	26.83243127616764	23.687746357419634
19	25.445702496646927	24.996323873434303	25.33920622401647	24.2187674059023
20	25.611750238373183	24.75717124551101	25.179336832443706	24.451741683672104
21	26.231180855377183	24.446508850957276	24.72364428642081	24.59866600724473
22	25.668281206136733	25.130447956723806	25.128442778909093	24.072828058230364
23	24.043420053150136	25.080360066560186	25.72234956573264	25.153870314557036
24	24.46064321805074	25.153497241950863	25.928558061612765	24.45730147838563
25	24.512509997081633	25.017766401340218	25.6027766824615	24.866946919116646
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	504.0
1	504.0
2	342.0
3	180.0
4	180.0
5	180.0
6	406.0
7	632.0
8	632.0
9	632.0
10	737.0
11	842.0
12	842.0
13	842.0
14	1036.5
15	1231.0
16	1231.0
17	1231.0
18	2039.0
19	2847.0
20	2847.0
21	2847.0
22	4668.0
23	6489.0
24	6489.0
25	6489.0
26	9758.5
27	13028.0
28	13028.0
29	13028.0
30	16726.5
31	20425.0
32	20425.0
33	20425.0
34	24645.5
35	28866.0
36	28866.0
37	28866.0
38	33009.0
39	37152.0
40	37152.0
41	37152.0
42	42696.0
43	48240.0
44	48240.0
45	48240.0
46	53016.5
47	57793.0
48	57793.0
49	57793.0
50	59281.5
51	60770.0
52	60770.0
53	60770.0
54	56298.5
55	51827.0
56	51827.0
57	51827.0
58	47566.5
59	43306.0
60	43306.0
61	43306.0
62	38207.0
63	33108.0
64	33108.0
65	33108.0
66	26885.0
67	20662.0
68	20662.0
69	20662.0
70	15524.0
71	10386.0
72	10386.0
73	10386.0
74	7897.5
75	5409.0
76	5409.0
77	5409.0
78	4326.0
79	3243.0
80	3243.0
81	3243.0
82	2258.0
83	1273.0
84	1273.0
85	1273.0
86	948.5
87	624.0
88	624.0
89	624.0
90	418.0
91	212.0
92	212.0
93	212.0
94	129.5
95	47.0
96	47.0
97	47.0
98	110.0
99	173.0
100	173.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.4516759447012813E-4
2	2.2258379723506406E-4
3	6.677513917051922E-4
4	0.008680768092167498
5	0.03271981819355441
6	0.06054279284793743
7	0.1028337143225996
8	0.10750797406453595
9	0.11262740140094243
10	0.12776309961292676
11	0.13599870011062415
12	0.13110185657145274
13	0.12286625607375537
14	0.1139629041843528
15	0.12865343480186703
16	0.11641132595393851
17	0.11685649354840864
18	0.10684022267283075
19	0.09504328141937236
20	0.08747543231338017
21	0.08703026471891005
22	0.09593361660831262
23	0.07834949662674255
24	0.08925610269126069
25	0.08636251332720486
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	449269.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.29967568263709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.17995449863213	50.574848371960165
2	12.062642143150795	15.029973867880098
3	3.179373966447384	5.942219009504745
4	1.2269166698790388	3.057460424923409
5	0.5963174827542599	1.857519288973846
6	0.346507299615074	1.295237543261128
7	0.2391763058979416	1.0430424401889073
8	0.15305445475591012	0.7628194314460841
9	0.11781079775225028	0.660561704269015
>10	0.7537922885649615	9.008327526225893
>50	0.08480389517626624	3.653952999756196
>100	0.05893152029722389	6.734583260489021
>500	3.593385383977067E-4	0.14730431756812074
>1k	3.593385383977067E-4	0.23214981355340608
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1037	0.23081939773276142	No Hit
TATCAACGCAGAGTACTTTTTTTTT	658	0.14646013858067217	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	494	0.10995639583412165	No Hit
GAATAGGACCGCGGTTCTATTTTGT	455	0.10127562774195416	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.2258379723506406E-4
13	0.0	0.0	0.0	0.0	4.4516759447012813E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAACT	25	0.0060181855	19.004015	5
GCGTTAT	35	0.0021735996	16.278265	1
CAAGACG	65	1.9022264E-7	16.078527	4
CGGTCCA	55	1.1236783E-5	15.547006	10
GCAAGAC	105	8.731149E-11	14.476021	3
GTTATTC	40	0.0052717696	14.249832	3
CGGACCA	70	7.227578E-6	13.5727825	9
AAGACTC	50	0.0014942668	13.302811	5
CCTAGAG	50	0.0014967807	13.299845	2
GGTTCTA	65	5.430553E-5	13.155158	13
CTTGCGC	80	1.9906365E-6	13.062346	3
GACGGAC	75	1.4698413E-5	12.669343	7
CGAAGAC	60	4.078512E-4	12.667931	4
CGTCGTA	75	1.4715282E-5	12.66793	10
GTAGAAC	60	4.0823512E-4	12.666519	3
CCTATTC	75	1.4732166E-5	12.666518	3
CCCTAGA	60	4.0977393E-4	12.660872	1
GTCCTAT	90	5.4062184E-7	12.660872	1
CGCAAGA	70	1.0876868E-4	12.214142	2
CTACACC	55	0.0030603092	12.092115	4
>>END_MODULE