FastQCFastQC Report
Thu 2 Feb 2017
SRR4062202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449269
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.11885974772352423No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT4760.10594988748389049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTG300.005741646529.3328885
CGAGCAA300.005741646529.33288831
GTATCAA6850.027.3140561
TCTAGCC655.9180456E-727.0765113
GGAGTGC508.33084E-526.39966
GGACGTG1750.023.8853536
ACTGATC651.8103163E-523.6919488
TAGGACG1950.022.563764
TGTAGGA2900.022.0021132
TATAACA956.847549E-720.841794
CCTATGC856.100161E-620.7055663
GGCGTTA1401.8371793E-1020.42826342
GTAGGAC2800.020.4282633
TATAGGA654.922341E-420.3096432
GATTTCG654.9260375E-420.30738441
TAGGAGT654.9260375E-420.3073844
CTAGCCT654.9260375E-420.3073844
TTACCCT550.004479696519.9996974
CTGTAGG3200.019.9482981
AGGACGT2050.019.316785
Produced by FastQC (version 0.11.5)