##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449269
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26027613745885	32.0	32.0	32.0	32.0	32.0
2	31.442966240715474	32.0	32.0	32.0	32.0	32.0
3	31.581422265947573	32.0	32.0	32.0	32.0	32.0
4	31.65427171694464	32.0	32.0	32.0	32.0	32.0
5	31.63986386774961	32.0	32.0	32.0	32.0	32.0
6	35.293574673525214	36.0	36.0	36.0	36.0	36.0
7	35.291206381922635	36.0	36.0	36.0	36.0	36.0
8	35.247226049426956	36.0	36.0	36.0	36.0	36.0
9	35.31407018957462	36.0	36.0	36.0	36.0	36.0
10	35.20461683312225	36.0	36.0	36.0	36.0	36.0
11	35.321849493287985	36.0	36.0	36.0	36.0	36.0
12	35.24171710044539	36.0	36.0	36.0	36.0	36.0
13	35.27365787534862	36.0	36.0	36.0	36.0	36.0
14	35.2379732409759	36.0	36.0	36.0	36.0	36.0
15	35.219391945582714	36.0	36.0	36.0	36.0	36.0
16	35.227469511584374	36.0	36.0	36.0	36.0	36.0
17	35.21141454228981	36.0	36.0	36.0	36.0	36.0
18	35.210321655845384	36.0	36.0	36.0	36.0	36.0
19	35.19667059156096	36.0	36.0	36.0	36.0	36.0
20	35.19168916617884	36.0	36.0	36.0	36.0	36.0
21	35.18138353636685	36.0	36.0	36.0	36.0	36.0
22	35.1624216226804	36.0	36.0	36.0	36.0	36.0
23	35.12955044750473	36.0	36.0	36.0	36.0	36.0
24	35.121279233599466	36.0	36.0	36.0	36.0	36.0
25	35.088347960798544	36.0	36.0	36.0	36.0	36.0
26	35.048258393078534	36.0	36.0	36.0	36.0	36.0
27	35.048280651458256	36.0	36.0	36.0	36.0	36.0
28	35.0139916174942	36.0	36.0	36.0	36.0	36.0
29	34.99288177016442	36.0	36.0	36.0	36.0	36.0
30	34.959456361333636	36.0	36.0	36.0	36.0	36.0
31	34.97514406736276	36.0	36.0	36.0	36.0	36.0
32	34.92887112175556	36.0	36.0	36.0	36.0	36.0
33	34.916829338325144	36.0	36.0	36.0	36.0	36.0
34	34.905152592322196	36.0	36.0	36.0	32.0	36.0
35	34.8925454460468	36.0	36.0	36.0	36.0	36.0
36	34.859353750203105	36.0	36.0	36.0	32.0	36.0
37	34.832999828610475	36.0	36.0	36.0	32.0	36.0
38	34.814171465202364	36.0	36.0	36.0	32.0	36.0
39	34.7727664272407	36.0	36.0	36.0	32.0	36.0
40	34.78254675929121	36.0	36.0	36.0	32.0	36.0
41	34.743901760415255	36.0	36.0	36.0	32.0	36.0
42	34.71270441539479	36.0	36.0	36.0	32.0	36.0
43	34.70386338696861	36.0	36.0	36.0	32.0	36.0
44	34.65318328217616	36.0	36.0	36.0	32.0	36.0
45	34.629264427325275	36.0	36.0	36.0	32.0	36.0
46	34.578904843200846	36.0	36.0	36.0	32.0	36.0
47	34.56307913521743	36.0	36.0	36.0	32.0	36.0
48	34.53788042353245	36.0	36.0	36.0	32.0	36.0
49	34.502629827564334	36.0	36.0	36.0	32.0	36.0
50	34.112829952656426	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	11.0
22	39.0
23	101.0
24	234.0
25	546.0
26	1115.0
27	2272.0
28	3812.0
29	5757.0
30	8660.0
31	12592.0
32	18878.0
33	31846.0
34	71767.0
35	291636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.876149189194	17.900966554670124	11.941770703218063	26.281113552917812
2	15.8776233427419	19.87774799882467	37.99272542716968	26.251903231263746
3	18.98973666110949	23.375527801829193	28.309988002733327	29.32474753432799
4	12.408378944463117	15.901163890675743	35.59493310243974	36.095524062421404
5	14.398500675541825	36.86521883326025	33.33437205772043	15.401908433477493
6	34.64813875014468	35.13248217099816	16.521986876430102	13.697392202427059
7	30.403388615729103	30.175685391157636	20.175440548980678	19.24548544413258
8	27.97945106383926	33.70052240417211	19.03314050157033	19.2868860304183
9	27.27363784280687	14.389152156057952	18.689248534842154	39.647961466293026
10	16.682692735739025	26.65313354167223	31.04182804027885	25.622345682309888
11	37.10405124769348	21.04195927161678	22.311577251045588	19.542412229644153
12	24.75332150671424	23.61391504866795	28.86689266341546	22.765870781202352
13	29.76969254500088	19.50791174107272	25.07985193725808	25.64254377666832
14	23.16340544306418	19.951966416556672	25.151969087562243	31.73265905281691
15	24.958766351562204	27.895759556078875	22.09811938949716	25.047354702861757
16	25.453683444366042	25.828293642755867	24.34654670830486	24.371476204573227
17	23.66199314887072	26.249974959322813	25.421740649811138	24.66629124199533
18	24.31916877936728	24.74653157253927	27.18094140010283	23.753358247990615
19	25.661685983230537	24.744640738622074	25.5281357048895	24.065537573257892
20	25.717115733475786	24.469299856432173	25.394143768154652	24.41944064193739
21	26.339676229608543	24.14410965368187	24.75554734468659	24.760666772022997
22	25.65434071792178	24.762892609995347	25.337158806861815	24.245607865221057
23	24.037492014806276	24.59150308612435	26.164947948779016	25.20605695029036
24	24.555272623668934	24.798336835357386	26.133631895722782	24.512758645250898
25	24.64876198616416	24.66189445943179	25.885885484833103	24.803458069570947
26	23.93454226551249	25.6515613209962	26.237181898514695	24.17671451497662
27	24.845526500885885	25.059875174728667	25.544218595582148	24.5503797288033
28	24.26168132768261	25.134442109761746	25.877435093842372	24.726441468713272
29	24.2129419411131	25.02960371092533	25.728740974206932	25.02871337375464
30	24.207646745892717	25.238223490472173	26.10030205024674	24.45382771338837
31	25.011574383218928	24.882252909176707	25.227926315695754	24.878246391908615
32	24.634071422847832	25.265988229742604	25.241949126134067	24.857991221275498
33	24.198696546382116	24.780086718840426	26.07441438072598	24.946802354051478
34	24.771793930085806	25.227427019687713	26.024729280046298	23.976049770180182
35	25.21880035969622	24.844190995129857	26.005858418583117	23.931150226590812
36	24.04350177733162	25.287300036281156	25.855333886825044	24.813864299562177
37	24.773462314946922	25.128933386457376	25.632424659943066	24.465179638652636
38	24.39073250101832	25.096768305847945	25.754503426677562	24.757995766456176
39	25.22920566520281	24.61131304407827	25.389020831617582	24.77046045910134
40	25.5884559139402	24.94006931259446	25.512109671488574	23.95936510197677
41	24.40547206097083	25.26487766658357	26.033690658499236	24.295959613946366
42	25.30985112899779	25.6453005912102	25.476796780397464	23.568051499394542
43	24.678354242162527	24.85108580484895	25.9593724680135	24.511187484975025
44	24.121919567632062	25.414972164533093	25.506235990446235	24.95687227738861
45	24.393783582853658	25.580618881632546	25.34801518935499	24.67758234615881
46	24.372692043155315	25.045797431782418	25.585572751696674	24.995937773365593
47	24.634235918667155	25.134831335625968	25.793907827228917	24.43702491847796
48	25.130994938275453	25.477124299394106	24.85720772658674	24.534673035743698
49	24.61575445680312	25.494983940150068	25.006399455100308	24.8828621479465
50	24.557557087433533	26.0706884770081	24.787709758339695	24.58404467721867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	138.5
2	29.0
3	102.5
4	176.0
5	162.5
6	149.0
7	102.0
8	55.0
9	97.5
10	140.0
11	225.0
12	310.0
13	581.0
14	852.0
15	1120.0
16	1388.0
17	1327.0
18	1266.0
19	1192.0
20	1118.0
21	1246.0
22	1374.0
23	1599.5
24	1825.0
25	2236.0
26	2647.0
27	3442.0
28	4237.0
29	5189.5
30	6142.0
31	7178.5
32	8215.0
33	9552.5
34	10890.0
35	12811.0
36	14732.0
37	16346.5
38	17961.0
39	19028.0
40	20095.0
41	21411.5
42	22728.0
43	23561.0
44	24394.0
45	26547.0
46	28700.0
47	30524.0
48	32348.0
49	33414.0
50	34480.0
51	33536.0
52	32592.0
53	32562.0
54	32532.0
55	32552.5
56	32573.0
57	31304.0
58	30035.0
59	27357.0
60	24679.0
61	21516.0
62	18353.0
63	15665.5
64	12978.0
65	10912.5
66	8847.0
67	7640.0
68	6433.0
69	5813.5
70	5194.0
71	4164.5
72	3135.0
73	2715.0
74	2295.0
75	1652.0
76	1009.0
77	913.0
78	817.0
79	691.5
80	566.0
81	448.0
82	330.0
83	264.5
84	199.0
85	168.5
86	138.0
87	87.5
88	37.0
89	23.5
90	10.0
91	8.0
92	6.0
93	5.0
94	4.0
95	4.5
96	5.0
97	3.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03294240199078948
2	0.007345265308757114
3	0.0
4	0.0
5	0.0
6	2.2258379723506406E-4
7	0.0
8	0.0
9	0.0
10	2.2258379723506406E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.4516759447012813E-4
17	0.0
18	4.4516759447012813E-4
19	0.0
20	8.903351889402563E-4
21	0.0
22	0.0
23	0.0
24	0.0011129189861753205
25	2.2258379723506406E-4
26	4.4516759447012813E-4
27	2.2258379723506406E-4
28	0.0011129189861753205
29	2.2258379723506406E-4
30	0.0013355027834103844
31	2.2258379723506406E-4
32	2.2258379723506406E-4
33	2.2258379723506406E-4
34	8.903351889402563E-4
35	2.2258379723506406E-4
36	0.0
37	0.0013355027834103844
38	0.0
39	0.0
40	0.0
41	0.0011129189861753205
42	0.004674259741936346
43	0.003783924552996089
44	0.0048968435391714095
45	0.0015580865806454486
46	0.0017806703778805125
47	8.903351889402563E-4
48	0.003338756958525961
49	0.002671005566820769
50	4.4516759447012813E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	449269.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.85197346651942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.34887776825555	52.756352625580405
2	12.253620110577017	15.893428925598993
3	3.077672365789085	5.987793798143814
4	1.169016098006101	3.03252003879303
5	0.5517858895910412	1.7892201935479013
6	0.3517461152224828	1.3686857838815842
7	0.2102552109070057	0.9544825751257006
8	0.16192151493221782	0.8400743832034254
9	0.11499490298447967	0.6711881757421007
>10	0.6448476207147158	8.053611900377083
>50	0.06936450010284352	3.1493449063417556
>100	0.04555280590312053	5.383786305928685
>500	3.4509701441757975E-4	0.11951038773553033
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	534	0.11885974772352423	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	476	0.10594988748389049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	4.4516759447012813E-4
13	0.0	0.0	0.0	0.0	6.677513917051922E-4
14	0.0	0.0	0.0	4.4516759447012813E-4	6.677513917051922E-4
15	0.0	0.0	0.0	6.677513917051922E-4	6.677513917051922E-4
16	0.0	0.0	0.0	6.677513917051922E-4	6.677513917051922E-4
17	0.0	0.0	0.0	6.677513917051922E-4	6.677513917051922E-4
18	0.0	0.0	0.0	0.0013355027834103844	6.677513917051922E-4
19	0.0	0.0	0.0	0.0015580865806454486	6.677513917051922E-4
20	0.0	0.0	0.0	0.002671005566820769	8.903351889402563E-4
21	0.0	0.0	0.0	0.003116173161290897	8.903351889402563E-4
22	0.0	0.0	0.0	0.003783924552996089	8.903351889402563E-4
23	0.0	0.0	0.0	0.005342011133641538	8.903351889402563E-4
24	0.0	0.0	0.0	0.00600976252534673	8.903351889402563E-4
25	0.0	0.0	0.0	0.008013016700462306	8.903351889402563E-4
26	0.0	0.0	0.0	0.010016270875577883	8.903351889402563E-4
27	0.0	0.0	0.0	0.011574357456223332	8.903351889402563E-4
28	0.0	0.0	0.0	0.012687276442398652	8.903351889402563E-4
29	0.0	0.0	0.0	0.015135698211984357	8.903351889402563E-4
30	0.0	0.0	0.0	0.019809957953920702	8.903351889402563E-4
31	0.0	0.0	0.0	0.030493980221203778	8.903351889402563E-4
32	0.0	0.0	0.0	0.037839245529960895	8.903351889402563E-4
33	0.0	0.0	0.0	0.048300684000008906	8.903351889402563E-4
34	0.0	0.0	0.0	0.05675886829494134	8.903351889402563E-4
35	0.0	0.0	0.0	0.06944614473734	8.903351889402563E-4
36	0.0	0.0	0.0	0.08257858877420877	8.903351889402563E-4
37	0.0	0.0	0.0	0.10238854672812947	8.903351889402563E-4
38	0.0	0.0	0.0	0.1282082672073969	8.903351889402563E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACGTG	30	0.0057416465	29.332888	5
CGAGCAA	30	0.0057416465	29.332888	31
GTATCAA	685	0.0	27.314056	1
TCTAGCC	65	5.9180456E-7	27.076511	3
GGAGTGC	50	8.33084E-5	26.3996	6
GGACGTG	175	0.0	23.885353	6
ACTGATC	65	1.8103163E-5	23.691948	8
TAGGACG	195	0.0	22.56376	4
TGTAGGA	290	0.0	22.002113	2
TATAACA	95	6.847549E-7	20.84179	4
CCTATGC	85	6.100161E-6	20.705566	3
GGCGTTA	140	1.8371793E-10	20.428263	42
GTAGGAC	280	0.0	20.428263	3
TATAGGA	65	4.922341E-4	20.309643	2
GATTTCG	65	4.9260375E-4	20.307384	41
TAGGAGT	65	4.9260375E-4	20.307384	4
CTAGCCT	65	4.9260375E-4	20.307384	4
TTACCCT	55	0.0044796965	19.999697	4
CTGTAGG	320	0.0	19.948298	1
AGGACGT	205	0.0	19.31678	5
>>END_MODULE