FastQCFastQC Report
Thu 2 Feb 2017
SRR4062200_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062200_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182552
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15760.13327109505543944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC851.7169441E-814.5282783
GGTATCA3050.014.0117411
GGGTTAA551.9650512E-413.8135961
CGCGTAA1051.366061E-913.57381810
TCGCGTA1051.366061E-913.5738189
GCGTAAC1051.366061E-913.57381811
GTATCAA10950.013.4430241
CGACCAT2250.013.09119310
GTCCTAT1750.013.0242461
CGAACGA604.093425E-412.66567916
CGGTCCA1155.304173E-912.39348610
AGAGAGT853.9332954E-612.2962836
GGACCGA701.0886809E-412.2164376
ACTGCCG550.00306365112.0930398
TAAGACT550.00306851412.0904794
AAGACGG2050.012.0498815
ATTTGCG951.0400381E-611.999063516
CTACACT1750.011.9424314
CGGACCA2100.011.7639769
CCGACCA2300.011.5672549
Produced by FastQC (version 0.11.5)