##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062200_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1182552
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332787902772985	32.0	32.0	32.0	32.0	32.0
2	30.908273801067523	32.0	32.0	32.0	32.0	32.0
3	30.927153309114527	32.0	32.0	32.0	32.0	32.0
4	30.93304734168138	32.0	32.0	32.0	32.0	32.0
5	30.85279294272049	32.0	32.0	32.0	32.0	32.0
6	34.52833025524459	36.0	36.0	36.0	32.0	36.0
7	34.47838065471962	36.0	36.0	36.0	32.0	36.0
8	34.44052016317253	36.0	36.0	36.0	32.0	36.0
9	34.57066243175776	36.0	36.0	36.0	32.0	36.0
10	34.285699064396326	36.0	36.0	36.0	32.0	36.0
11	34.54783299169931	36.0	36.0	36.0	32.0	36.0
12	34.39358607486182	36.0	36.0	36.0	32.0	36.0
13	34.46405824014504	36.0	36.0	36.0	32.0	36.0
14	34.38121029772898	36.0	36.0	36.0	32.0	36.0
15	34.32700380194697	36.0	36.0	36.0	32.0	36.0
16	34.34078670536264	36.0	36.0	36.0	32.0	36.0
17	34.2710434720841	36.0	36.0	36.0	32.0	36.0
18	34.280272664542444	36.0	36.0	36.0	32.0	36.0
19	34.283681394137425	36.0	36.0	36.0	32.0	36.0
20	34.27219606410543	36.0	36.0	36.0	32.0	36.0
21	34.25393132817838	36.0	36.0	36.0	32.0	36.0
22	34.231742874731935	36.0	36.0	36.0	32.0	36.0
23	34.19736975625596	36.0	36.0	36.0	32.0	36.0
24	34.171886733099264	36.0	36.0	36.0	32.0	36.0
25	33.82465126269289	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	13.0
5	83.0
6	258.0
7	61.0
8	211.0
9	160.0
10	75.0
11	22.0
12	51.0
13	53.0
14	140.0
15	239.0
16	354.0
17	471.0
18	678.0
19	872.0
20	1285.0
21	1906.0
22	2801.0
23	4166.0
24	6114.0
25	8624.0
26	11938.0
27	15660.0
28	21180.0
29	28322.0
30	37009.0
31	51378.0
32	73592.0
33	106405.0
34	241418.0
35	567012.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.94622934849617	16.517482269113714	11.345822259016812	26.190466123373312
2	17.230520224566128	18.690673605361987	36.72809621943145	27.35070995064044
3	19.836132947272997	21.967131878390994	27.470182927654278	30.72655224668173
4	13.35828185058798	14.688756675842646	33.87721100948467	38.075750464084706
5	15.63654143199617	35.51694618467644	32.843521233274174	16.00299115005321
6	36.43253031418441	33.5449614567732	15.88488648575466	14.137621743287724
7	31.88731888842613	29.42684260604275	19.313121975830345	19.372716529700774
8	28.831565286515016	32.66040040736611	18.360173613139334	20.14786069297954
9	26.923952862847955	14.708559208025406	18.027869472860747	40.33961845626589
10	16.6102157047705	26.445130511801356	30.35464187792028	26.590011905507865
11	37.660672625423935	21.246427497787696	21.396398494362327	19.69650138242604
12	24.994728994088852	23.107920564882484	27.83844726750207	24.058903173526595
13	29.67505076008894	19.283414871224323	24.57829754302542	26.46323682566132
14	23.897176789513473	18.86878552083395	24.415983094771047	32.81805459488153
15	25.495980504558023	26.761524614011588	21.42796661117669	26.3145282702537
16	26.372033561081338	25.678291127987606	22.702920085003345	25.246755225927714
17	24.572844239281736	25.847114645388753	24.419508444899023	25.16053267043049
18	25.376852941425465	24.97295192299552	24.965332746374543	24.684862389204472
19	25.944914648092144	24.716798586087897	24.601427286031946	24.736859479788016
20	26.1501987391743	24.279020320826646	24.295525490753718	25.275255449245332
21	26.52306874536056	24.10730231656604	24.30604165908416	25.063587278989242
22	26.254476873472615	23.811930259389904	24.588161500115547	25.345431367021938
23	25.10697613010464	24.076738446747214	24.925861479452795	25.89042394369535
24	25.118096306998765	24.481336932297655	24.800436073946358	25.60013068675722
25	25.336908516282147	24.15711507112278	24.683489662947597	25.822486749647478
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	56.0
2	179.0
3	302.0
4	302.0
5	302.0
6	607.0
7	912.0
8	912.0
9	912.0
10	1190.5
11	1469.0
12	1469.0
13	1469.0
14	2002.0
15	2535.0
16	2535.0
17	2535.0
18	4285.0
19	6035.0
20	6035.0
21	6035.0
22	9363.0
23	12691.0
24	12691.0
25	12691.0
26	18781.0
27	24871.0
28	24871.0
29	24871.0
30	32173.0
31	39475.0
32	39475.0
33	39475.0
34	50543.0
35	61611.0
36	61611.0
37	61611.0
38	75172.5
39	88734.0
40	88734.0
41	88734.0
42	104432.0
43	120130.0
44	120130.0
45	120130.0
46	135542.5
47	150955.0
48	150955.0
49	150955.0
50	157826.0
51	164697.0
52	164697.0
53	164697.0
54	158335.0
55	151973.0
56	151973.0
57	151973.0
58	141159.0
59	130345.0
60	130345.0
61	130345.0
62	114645.5
63	98946.0
64	98946.0
65	98946.0
66	81268.5
67	63591.0
68	63591.0
69	63591.0
70	48133.0
71	32675.0
72	32675.0
73	32675.0
74	24540.0
75	16405.0
76	16405.0
77	16405.0
78	12511.0
79	8617.0
80	8617.0
81	8617.0
82	5919.0
83	3221.0
84	3221.0
85	3221.0
86	2272.5
87	1324.0
88	1324.0
89	1324.0
90	871.0
91	418.0
92	418.0
93	418.0
94	262.5
95	107.0
96	107.0
97	107.0
98	282.0
99	457.0
100	457.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.301916533057321E-4
2	4.228143878662418E-4
3	6.76503020585987E-4
4	0.009893856676070059
5	0.03365602527415285
6	0.06443691271081525
7	0.10451971668053497
8	0.11035455523308911
9	0.11864171723526747
10	0.1341167238311719
11	0.14012068813887255
12	0.1321717776469872
13	0.12735169362531204
14	0.1166122081735095
15	0.13174896325912094
16	0.12024841190915918
17	0.12549131031870056
18	0.11179212415183433
19	0.09716274633166237
20	0.0933574168408662
21	0.09344197971843944
22	0.10172914172061778
23	0.0828716200217834
24	0.09310372820814646
25	0.09082053051366874
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1182552.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.482247902437464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.53313566745685	41.15215022282826
2	13.560607659678844	14.776247764446302
3	4.530769777146916	7.405395665621689
4	2.0749048432257666	4.521819201703774
5	1.1161753232599507	3.0405870332215956
6	0.7049678462315255	2.304493977698004
7	0.4750595188447673	1.8117617331879294
8	0.3334511798582622	1.4533735875518483
9	0.2442721080545344	1.197764419201032
>10	1.2943398247666575	12.384031245330313
>50	0.07788970903364961	2.89908967285872
>100	0.05005994702352494	5.324078275361776
>500	0.004054695746055779	1.5032003635954374
>1k	3.1189967277352144E-4	0.22600683739339042
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1576	0.13327109505543944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.6912575514649672E-4
13	0.0	0.0	0.0	0.0	1.6912575514649672E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	85	1.7169441E-8	14.528278	3
GGTATCA	305	0.0	14.011741	1
GGGTTAA	55	1.9650512E-4	13.813596	1
CGCGTAA	105	1.366061E-9	13.573818	10
TCGCGTA	105	1.366061E-9	13.573818	9
GCGTAAC	105	1.366061E-9	13.573818	11
GTATCAA	1095	0.0	13.443024	1
CGACCAT	225	0.0	13.091193	10
GTCCTAT	175	0.0	13.024246	1
CGAACGA	60	4.093425E-4	12.665679	16
CGGTCCA	115	5.304173E-9	12.393486	10
AGAGAGT	85	3.9332954E-6	12.296283	6
GGACCGA	70	1.0886809E-4	12.216437	6
ACTGCCG	55	0.003063651	12.093039	8
TAAGACT	55	0.003068514	12.090479	4
AAGACGG	205	0.0	12.049881	5
ATTTGCG	95	1.0400381E-6	11.9990635	16
CTACACT	175	0.0	11.942431	4
CGGACCA	210	0.0	11.763976	9
CCGACCA	230	0.0	11.567254	9
>>END_MODULE