##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1182552
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300661619954134	32.0	32.0	32.0	32.0	32.0
2	31.49231154317104	32.0	32.0	32.0	32.0	32.0
3	31.59872039453656	32.0	32.0	32.0	32.0	32.0
4	31.669864834696487	32.0	32.0	32.0	32.0	32.0
5	31.647003260744558	32.0	32.0	32.0	32.0	32.0
6	35.30878642123137	36.0	36.0	36.0	36.0	36.0
7	35.31129540180897	36.0	36.0	36.0	36.0	36.0
8	35.266690175146636	36.0	36.0	36.0	36.0	36.0
9	35.345779297654566	36.0	36.0	36.0	36.0	36.0
10	35.235440809368214	36.0	36.0	36.0	36.0	36.0
11	35.343179834797965	36.0	36.0	36.0	36.0	36.0
12	35.2729529018597	36.0	36.0	36.0	36.0	36.0
13	35.30604235585412	36.0	36.0	36.0	36.0	36.0
14	35.26820131376886	36.0	36.0	36.0	36.0	36.0
15	35.248457573113065	36.0	36.0	36.0	36.0	36.0
16	35.258511253627745	36.0	36.0	36.0	36.0	36.0
17	35.24740814780238	36.0	36.0	36.0	36.0	36.0
18	35.24163673140801	36.0	36.0	36.0	36.0	36.0
19	35.23628221000007	36.0	36.0	36.0	36.0	36.0
20	35.232414303979866	36.0	36.0	36.0	36.0	36.0
21	35.22863011520847	36.0	36.0	36.0	36.0	36.0
22	35.2127356767398	36.0	36.0	36.0	36.0	36.0
23	35.18455678904606	36.0	36.0	36.0	36.0	36.0
24	35.16039717492339	36.0	36.0	36.0	36.0	36.0
25	35.15173286248723	36.0	36.0	36.0	36.0	36.0
26	35.10707605246957	36.0	36.0	36.0	36.0	36.0
27	35.10012160141795	36.0	36.0	36.0	36.0	36.0
28	35.07920412802143	36.0	36.0	36.0	36.0	36.0
29	35.06686302166839	36.0	36.0	36.0	36.0	36.0
30	35.04565803448813	36.0	36.0	36.0	36.0	36.0
31	35.047137884845654	36.0	36.0	36.0	36.0	36.0
32	35.02985238704091	36.0	36.0	36.0	36.0	36.0
33	35.005250509068524	36.0	36.0	36.0	36.0	36.0
34	34.99722041821417	36.0	36.0	36.0	36.0	36.0
35	34.995004870821745	36.0	36.0	36.0	36.0	36.0
36	34.964129272962204	36.0	36.0	36.0	36.0	36.0
37	34.95401555280444	36.0	36.0	36.0	36.0	36.0
38	34.93804162523086	36.0	36.0	36.0	36.0	36.0
39	34.930643219071975	36.0	36.0	36.0	36.0	36.0
40	34.910948524885164	36.0	36.0	36.0	36.0	36.0
41	34.89457123238555	36.0	36.0	36.0	36.0	36.0
42	34.871539687049705	36.0	36.0	36.0	36.0	36.0
43	34.862651282987976	36.0	36.0	36.0	32.0	36.0
44	34.8207165519994	36.0	36.0	36.0	32.0	36.0
45	34.81794035272868	36.0	36.0	36.0	32.0	36.0
46	34.79925703055764	36.0	36.0	36.0	32.0	36.0
47	34.777415284909246	36.0	36.0	36.0	32.0	36.0
48	34.75725042112313	36.0	36.0	36.0	32.0	36.0
49	34.74061774873325	36.0	36.0	36.0	32.0	36.0
50	34.341122419986604	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	30.0
22	82.0
23	204.0
24	514.0
25	1262.0
26	2613.0
27	4936.0
28	8507.0
29	13526.0
30	20132.0
31	29835.0
32	45229.0
33	76899.0
34	180953.0
35	797824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67464730830981	17.04567947392729	11.746210858793058	26.533462358969846
2	16.75229027290463	19.122774526194906	36.378320187467075	27.74661501343339
3	19.27010089239582	22.327738347820425	27.682113269070914	30.720047490712844
4	13.07426650159993	15.116037180606012	34.02345097720861	37.786245340585445
5	15.355688375648596	35.779060878506826	32.666216792157975	16.1990339536866
6	36.54568517187434	33.70039321804575	15.715783687793328	14.038137922286584
7	31.869380796785258	29.365558554718945	19.339530101001902	19.425530547493892
8	28.947225999364086	32.67061406179179	18.289005472909437	20.09315446593469
9	27.08633531548718	14.457884304453419	18.00402857548759	40.451751804571806
10	16.61122151283244	26.48708299860471	30.3057798824574	26.595915606105454
11	37.87241491283259	21.093617870503792	21.36117481514555	19.672792401518073
12	25.0723858232027	22.94681333252153	27.88308674798233	24.09771409629344
13	29.767739600457315	19.17657743591825	24.678661065221657	26.377021898402774
14	23.973998606403775	18.69778242309852	24.427847570339402	32.9003714001583
15	25.477526569656135	26.8043181187804	21.498335802569358	26.219819508994107
16	26.499389453958738	25.57934223388818	22.669692900415036	25.25157541173804
17	24.692613940021243	25.690117643875283	24.416769833377305	25.200498582726173
18	25.43050960972541	24.939537542535128	25.021901785291472	24.608051062447995
19	25.93729493502189	24.65109356713278	24.70868088676016	24.70293061108518
20	26.28554709268353	24.034565951066806	24.410617385634954	25.26926957061471
21	26.694048126424885	23.92774271237121	24.315970883309994	25.06223827789391
22	26.32915930969632	23.693588104370885	24.629783721984317	25.34746886394848
23	25.22180842787463	23.935860748618243	24.960509136173293	25.881821687333833
24	25.276311539040474	24.377146858208842	24.80977419829486	25.536767404455823
25	25.49980973320367	23.974884782884445	24.87911716206503	25.646188321846857
26	25.214600712869277	24.69766478231272	24.979599085024248	25.108135419793747
27	25.617204868466338	24.316286259596854	24.65115610431365	25.415352767623162
28	25.43582145844062	24.116201015274786	24.96285528486635	25.48512224141824
29	25.148239225807323	24.577776124098133	25.052936540419502	25.221048109675042
30	25.332759425505863	24.53675828896541	24.987315396740563	25.143166888788166
31	25.58724988287982	24.30899093988733	24.45029622526312	25.653462951969736
32	25.68564044974225	24.270809373689268	24.284508652532168	25.759041524036313
33	25.135512698871754	24.328525063718452	24.716247824608935	25.819714412800852
34	25.748135369628088	24.222690141559696	24.77066314881526	25.258511339996954
35	25.862628831979762	24.19487411927465	24.97945115124291	24.96304589750268
36	25.13699232504727	24.635279075352543	24.62276372713835	25.60496487246184
37	26.00505019754289	24.22936285652433	24.680345835362882	25.0852411105699
38	25.285315148932142	24.13424525940508	24.86148600653502	25.718953585127757
39	25.945168873780165	24.26700153905999	24.247467316116158	25.540362271043687
40	25.846073446303798	24.29003062024386	24.682910081679353	25.18098585177299
41	25.05885636003856	24.725928932636528	24.939959747661813	25.275254959663094
42	26.02639866585933	24.492170718589005	24.598472368336484	24.882958247215186
43	25.66883494685853	23.923217173049732	24.693024294209575	25.71492358588216
44	25.236573052236345	24.483091052084127	24.635140506211364	25.645195389468167
45	25.292404955176274	24.551276467817484	24.753133348160414	25.40318522884583
46	25.33739244413437	24.448700873131646	24.567007040020293	25.646899642713684
47	25.454425527488784	24.442279607318838	24.910510199680854	25.192784665511525
48	25.835809712593427	24.921142049173532	24.060850431202883	25.182197807030153
49	25.3641652128247	24.71942893820629	24.361542800459027	25.55486304850998
50	25.327603236456138	24.93412418141473	24.478574714509932	25.259697867619202
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	32.0
2	37.0
3	101.5
4	166.0
5	181.5
6	197.0
7	152.0
8	107.0
9	152.5
10	198.0
11	342.5
12	487.0
13	895.5
14	1304.0
15	1619.5
16	1935.0
17	1832.0
18	1729.0
19	1670.5
20	1612.0
21	1875.5
22	2139.0
23	2757.0
24	3375.0
25	4241.5
26	5108.0
27	6444.0
28	7780.0
29	9136.0
30	10492.0
31	12553.5
32	14615.0
33	17128.5
34	19642.0
35	23323.5
36	27005.0
37	31530.5
38	36056.0
39	41173.5
40	46291.0
41	50968.0
42	55645.0
43	59499.0
44	63353.0
45	68954.5
46	74556.0
47	79447.5
48	84339.0
49	89050.5
50	93762.0
51	93767.5
52	93773.0
53	94057.0
54	94341.0
55	93778.5
56	93216.0
57	89602.0
58	85988.0
59	79935.5
60	73883.0
61	65485.0
62	57087.0
63	50024.5
64	42962.0
65	36440.0
66	29918.0
67	25868.5
68	21819.0
69	18681.5
70	15544.0
71	11881.0
72	8218.0
73	7006.0
74	5794.0
75	4432.0
76	3070.0
77	2575.0
78	2080.0
79	1695.0
80	1310.0
81	1027.0
82	744.0
83	599.5
84	455.0
85	345.5
86	236.0
87	166.0
88	96.0
89	56.5
90	17.0
91	12.5
92	8.0
93	11.0
94	14.0
95	12.5
96	11.0
97	10.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030273510171222914
2	0.0043127067562356665
3	4.228143878662418E-4
4	0.0
5	0.0
6	1.6912575514649674E-4
7	0.0
8	0.0
9	0.0
10	1.6912575514649674E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.382515102929935E-4
17	0.0
18	0.0
19	0.0
20	8.456287757324837E-5
21	0.0
22	0.0
23	0.0
24	8.456287757324836E-4
25	1.6912575514649674E-4
26	5.919401430127385E-4
27	2.536886327197451E-4
28	0.0012684431635987255
29	3.382515102929935E-4
30	0.001353006041171974
31	5.073772654394902E-4
32	6.76503020585987E-4
33	5.073772654394902E-4
34	0.0010147545308789804
35	3.382515102929935E-4
36	3.382515102929935E-4
37	0.002029509061757961
38	0.0
39	0.0010147545308789804
40	8.456287757324837E-5
41	0.0010147545308789804
42	0.005750275674980889
43	0.004059018123515922
44	0.003551640858076431
45	0.002621449204770699
46	0.0022831976944777056
47	0.0012684431635987255
48	0.003889892368369425
49	0.004481832511382163
50	0.002029509061757961
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1182552.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.83030137845667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.44548986273544	42.12144436681818
2	13.804137311994257	15.413782927964764
3	4.611787381598187	7.724324382239711
4	1.9949621221313978	4.455173460688057
5	1.110551094658192	3.1001201155470914
6	0.6888411536642696	2.307492552657597
7	0.44735077814567853	1.7483010136032238
8	0.3122772254417504	1.3947625288032943
9	0.23776163861811778	1.1946873546256696
>10	1.2338995215892214	11.979700946407975
>50	0.06633126832744787	2.5287793058990653
>100	0.043259554316905334	4.655963318644096
>500	0.0030464425265586993	1.190671672631629
>1k	3.0464425265586993E-4	0.18479605346964717
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.536886327197451E-4
2	0.0	0.0	0.0	0.0	2.536886327197451E-4
3	0.0	0.0	0.0	0.0	2.536886327197451E-4
4	0.0	0.0	0.0	0.0	2.536886327197451E-4
5	0.0	0.0	0.0	0.0	2.536886327197451E-4
6	0.0	0.0	0.0	0.0	2.536886327197451E-4
7	0.0	0.0	0.0	0.0	2.536886327197451E-4
8	0.0	0.0	0.0	0.0	2.536886327197451E-4
9	0.0	0.0	0.0	0.0	2.536886327197451E-4
10	0.0	0.0	0.0	0.0	2.536886327197451E-4
11	0.0	0.0	0.0	0.0	2.536886327197451E-4
12	0.0	0.0	0.0	0.0	3.3825151029299343E-4
13	0.0	0.0	0.0	0.0	4.228143878662418E-4
14	0.0	0.0	0.0	0.0	4.228143878662418E-4
15	0.0	0.0	0.0	1.6912575514649672E-4	4.228143878662418E-4
16	0.0	0.0	0.0	1.6912575514649672E-4	4.228143878662418E-4
17	0.0	0.0	0.0	1.6912575514649672E-4	4.228143878662418E-4
18	0.0	0.0	0.0	2.536886327197451E-4	4.228143878662418E-4
19	0.0	0.0	0.0	3.3825151029299343E-4	4.228143878662418E-4
20	0.0	0.0	0.0	4.228143878662418E-4	4.228143878662418E-4
21	0.0	0.0	0.0	7.610658981592353E-4	4.228143878662418E-4
22	0.0	0.0	0.0	0.0010147545308789804	4.228143878662418E-4
23	0.0	0.0	0.0	0.0014375689187452222	4.228143878662418E-4
24	0.0	0.0	0.0	0.002029509061757961	4.228143878662418E-4
25	0.0	0.0	0.0	0.0027060120823439475	4.228143878662418E-4
26	0.0	0.0	0.0	0.004059018123515922	5.073772654394902E-4
27	0.0	0.0	0.0	0.004228143878662418	5.073772654394902E-4
28	0.0	0.0	0.0	0.006342215817993628	5.073772654394902E-4
29	0.0	0.0	0.0	0.008033473369458595	5.073772654394902E-4
30	0.0	0.0	0.0	0.011077736962095536	5.073772654394902E-4
31	0.0	0.0	0.0	0.015644132351050947	5.073772654394902E-4
32	0.0	0.0	0.0	0.021478970903605083	5.073772654394902E-4
33	0.0	0.0	0.0	0.028244001109464952	5.073772654394902E-4
34	0.0	0.0	0.0	0.0363620373564968	5.919401430127386E-4
35	0.0	0.0	0.0	0.044310947848382146	5.919401430127386E-4
36	0.0	0.0	0.0	0.05454305603474519	5.919401430127386E-4
37	0.0	0.0	0.0	0.07137106867182162	5.919401430127386E-4
38	0.0	0.0	0.0	0.09251178806513372	5.919401430127386E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	545	0.0	29.071064	1
GTATCAA	1460	0.0	28.033997	1
AGCGCTA	45	0.0013986891	24.442965	14
TACGAAT	210	0.0	22.00332	41
ATACGAA	210	0.0	22.002392	40
TACACCG	95	6.867249E-7	20.841724	5
CGCAATA	230	0.0	20.089138	36
TCAACGC	2015	0.0	20.088963	4
ACTATAC	90	1.00245325E-5	19.554373	3
CAACGCA	2060	0.0	19.543331	5
ATCAACG	2085	0.0	19.413694	3
TATCAAC	2120	0.0	19.300716	2
TAAACGC	150	4.656613E-10	19.06632	28
AACGCAG	2130	0.0	19.004349	6
GTCTAGG	175	9.094947E-12	18.861584	1
AGCTACG	70	8.1241777E-4	18.856798	7
AATACGA	250	0.0	18.482006	39
AACGTCG	60	0.0074109817	18.333773	22
TGTACGC	60	0.0074145976	18.332224	2
CAATACG	255	0.0	18.119616	38
>>END_MODULE