FastQCFastQC Report
Thu 2 Feb 2017
SRR4062199_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062199_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713491
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51930.13984145915528004No Hit
GCGCAAGACGGACCAGAGCGAAAGC38680.10416074793233644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15450.013.7678331
GTATTAG2350.013.7390631
GTATCAA41650.012.6766881
CAAGACG8850.011.7005154
AAGACGG8700.011.6846485
CGTTATT2950.011.5908262
TATACCG1001.930297E-611.400725
CGCGTAA3300.011.2288910
CGTCTTA1201.27764E-711.08298815
CGTCGTA5150.011.06955510
CGCAAGA8750.010.96344952
ACGAACG2700.010.90706615
TCGCGTA3400.010.8986299
GTCCTAG2800.010.8527051
GCGTTAT3150.010.8527051
CGCCAGT5600.010.6865918
CCGTCGT5450.010.6345619
GCGTAAC3600.010.557219511
ACGGTAT5150.010.5160789
CGCATCG5900.010.46648113
Produced by FastQC (version 0.11.5)