##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062199_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3713491
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271845549107297	32.0	32.0	32.0	32.0	32.0
2	30.88256979753014	32.0	32.0	32.0	32.0	32.0
3	30.909375032819522	32.0	32.0	32.0	32.0	32.0
4	30.91137853841574	32.0	32.0	32.0	32.0	32.0
5	30.85932832474887	32.0	32.0	32.0	32.0	32.0
6	34.48428150223065	36.0	36.0	36.0	32.0	36.0
7	34.43523250763231	36.0	36.0	36.0	32.0	36.0
8	34.403464018089714	36.0	36.0	36.0	32.0	36.0
9	34.511820548373485	36.0	36.0	36.0	32.0	36.0
10	34.259911495678864	36.0	36.0	36.0	32.0	36.0
11	34.48004748092832	36.0	36.0	36.0	32.0	36.0
12	34.34152822775119	36.0	36.0	36.0	32.0	36.0
13	34.41324187940673	36.0	36.0	36.0	32.0	36.0
14	34.34008807345972	36.0	36.0	36.0	32.0	36.0
15	34.29738055107714	36.0	36.0	36.0	32.0	36.0
16	34.29622799678254	36.0	36.0	36.0	32.0	36.0
17	34.24254024043683	36.0	36.0	36.0	32.0	36.0
18	34.24074004757249	36.0	36.0	36.0	32.0	36.0
19	34.244266917571636	36.0	36.0	36.0	32.0	36.0
20	34.230804383260924	36.0	36.0	36.0	32.0	36.0
21	34.21317272614906	36.0	36.0	36.0	32.0	36.0
22	34.189113963114494	36.0	36.0	36.0	32.0	36.0
23	34.16333444728963	36.0	36.0	36.0	32.0	36.0
24	34.144232744875374	36.0	36.0	36.0	32.0	36.0
25	33.79647803104949	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	58.0
5	302.0
6	750.0
7	157.0
8	667.0
9	521.0
10	277.0
11	84.0
12	154.0
13	164.0
14	443.0
15	617.0
16	1120.0
17	1607.0
18	2279.0
19	3155.0
20	4796.0
21	6627.0
22	10166.0
23	14556.0
24	20742.0
25	28939.0
26	39654.0
27	50941.0
28	67738.0
29	90114.0
30	117418.0
31	161514.0
32	229403.0
33	330985.0
34	755228.0
35	1772315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31064357368831	17.17326627623031	11.672750852845278	25.8433392972361
2	17.003174647628654	19.22235809346643	37.055750625490944	26.71871663341397
3	19.400893451745716	22.717958950984567	27.916556594208696	29.964591003061024
4	13.32853769081464	15.503840060025428	34.43099863106041	36.736623618099514
5	15.47678471517532	35.83801762336449	32.801164582699734	15.884033078760455
6	35.31016776654472	34.054417893661	16.41321103943259	14.222203300361691
7	31.13379800427828	29.80138484701848	19.86639474744806	19.19842240125518
8	28.37030209524847	32.88594656861648	18.78277658905379	19.960974747081263
9	27.065861967164057	14.771484303911114	18.4213726174797	39.74128111144513
10	16.92414185125422	26.444566568009044	30.31095487923789	26.320336701498846
11	37.29816287631587	21.215671913074527	21.614168429432382	19.871996781177224
12	24.722615390298827	23.375702006132435	27.951351483991143	23.950331119577594
13	29.37781081753721	19.403919957138193	24.85008748435757	26.368181740967028
14	23.85886310131708	19.28129350776521	24.533769305180652	32.326074085737055
15	25.42422196311329	26.707997657233403	21.71858972353844	26.149190656114868
16	26.151123022190824	25.654164975943424	23.005231917799172	25.189480084066577
17	24.34700242478593	26.004177652980857	24.54261925953568	25.106200662697525
18	25.15798333464537	25.109671707395453	25.16488499568107	24.56745996227811
19	25.81964064631585	24.916741400609784	24.721449275127924	24.54216867794644
20	25.972435683531113	24.306215556059897	24.267347778202456	25.454000982206537
21	26.699176587250783	24.123279300774357	24.15893922637638	25.018604885598485
22	26.249432553986896	24.036770501649233	24.49282996244343	25.22096698192044
23	24.869342063985737	24.23491800998908	25.0091902549878	25.886549671037386
24	24.947635321353765	24.7459932361897	24.821787587677647	25.484583854778887
25	25.17423182880013	24.315801304693647	24.719559326958557	25.790407539547665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	310.0
1	310.0
2	690.0
3	1070.0
4	1070.0
5	1070.0
6	2133.0
7	3196.0
8	3196.0
9	3196.0
10	3984.5
11	4773.0
12	4773.0
13	4773.0
14	6621.5
15	8470.0
16	8470.0
17	8470.0
18	14240.5
19	20011.0
20	20011.0
21	20011.0
22	31783.0
23	43555.0
24	43555.0
25	43555.0
26	64475.5
27	85396.0
28	85396.0
29	85396.0
30	110289.5
31	135183.0
32	135183.0
33	135183.0
34	172941.5
35	210700.0
36	210700.0
37	210700.0
38	251201.0
39	291702.0
40	291702.0
41	291702.0
42	338895.0
43	386088.0
44	386088.0
45	386088.0
46	432205.0
47	478322.0
48	478322.0
49	478322.0
50	495830.0
51	513338.0
52	513338.0
53	513338.0
54	486939.5
55	460541.0
56	460541.0
57	460541.0
58	425590.0
59	390639.0
60	390639.0
61	390639.0
62	344102.5
63	297566.0
64	297566.0
65	297566.0
66	241357.5
67	185149.0
68	185149.0
69	185149.0
70	141813.5
71	98478.0
72	98478.0
73	98478.0
74	74355.0
75	50232.0
76	50232.0
77	50232.0
78	39634.0
79	29036.0
80	29036.0
81	29036.0
82	20128.0
83	11220.0
84	11220.0
85	11220.0
86	8058.5
87	4897.0
88	4897.0
89	4897.0
90	3339.5
91	1782.0
92	1782.0
93	1782.0
94	1106.0
95	430.0
96	430.0
97	430.0
98	918.5
99	1407.0
100	1407.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010502247076941886
2	2.154307092706028E-4
3	7.540074824471097E-4
4	0.010717677786212488
5	0.03393033671011994
6	0.06252876336579247
7	0.10922336960019562
8	0.11035438082386628
9	0.11972561667713749
10	0.13580213335645624
11	0.14280363140775082
12	0.13496733935803262
13	0.13001243304480878
14	0.1180290998416315
15	0.13612527942036212
16	0.12187992376984352
17	0.1268348300830674
18	0.11415134707476066
19	0.09866726484593608
20	0.09363157201673573
21	0.09274292034099449
22	0.10467239586685413
23	0.08183674068417024
24	0.0929583510502651
25	0.09015775182974727
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3713491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.20460658510144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.5041390312856	30.967026543603577
2	16.418751018817655	14.844063608487707
3	6.3979988519917095	8.676570631086475
4	3.0074445445014497	5.438013898427905
5	1.5869153887520575	3.5867942916190025
6	0.9420466365972057	2.5550908555316822
7	0.6418714424271632	2.031088222319205
8	0.43868495423317344	1.5864464616731067
9	0.33762737412664456	1.373608135778024
>10	1.5497116316119104	12.275900336284678
>50	0.09285281373571948	2.908853772136545
>100	0.06572862671225936	6.007675839181979
>500	0.01041925177305001	3.2664264939092664
>1k	0.0057485526772790615	4.341872316621594
>5k	5.988075705499022E-5	0.14056859333929303
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5193	0.13984145915528004	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3868	0.10416074793233644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3464419329412675E-4
2	0.0	0.0	0.0	0.0	1.3464419329412675E-4
3	0.0	0.0	0.0	0.0	1.3464419329412675E-4
4	0.0	0.0	0.0	0.0	1.3464419329412675E-4
5	0.0	0.0	0.0	0.0	1.615730319529521E-4
6	0.0	0.0	0.0	0.0	2.9621722524707884E-4
7	0.0	0.0	0.0	0.0	2.9621722524707884E-4
8	0.0	0.0	0.0	0.0	2.9621722524707884E-4
9	0.0	0.0	0.0	0.0	2.9621722524707884E-4
10	0.0	0.0	0.0	0.0	3.231460639059042E-4
11	0.0	0.0	0.0	0.0	3.231460639059042E-4
12	0.0	0.0	0.0	0.0	8.347939984235858E-4
13	0.0	0.0	0.0	0.0	0.0010502247076941886
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1545	0.0	13.767833	1
GTATTAG	235	0.0	13.739063	1
GTATCAA	4165	0.0	12.676688	1
CAAGACG	885	0.0	11.700515	4
AAGACGG	870	0.0	11.684648	5
CGTTATT	295	0.0	11.590826	2
TATACCG	100	1.930297E-6	11.40072	5
CGCGTAA	330	0.0	11.22889	10
CGTCTTA	120	1.27764E-7	11.082988	15
CGTCGTA	515	0.0	11.069555	10
CGCAAGA	875	0.0	10.9634495	2
ACGAACG	270	0.0	10.907066	15
TCGCGTA	340	0.0	10.898629	9
GTCCTAG	280	0.0	10.852705	1
GCGTTAT	315	0.0	10.852705	1
CGCCAGT	560	0.0	10.68659	18
CCGTCGT	545	0.0	10.634561	9
GCGTAAC	360	0.0	10.5572195	11
ACGGTAT	515	0.0	10.516078	9
CGCATCG	590	0.0	10.466481	13
>>END_MODULE