FastQCFastQC Report
Thu 2 Feb 2017
SRR4062199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713491
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT38430.1034875269658658No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA38360.10329902509525403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41400.026.7402111
GGTATCA20400.025.245441
CGCAATA8000.017.32496336
TCAACGC63900.016.9726734
ATCAACG65200.016.7354853
CAACGCA65750.016.5285745
AACGCAG66550.016.4951656
TACCGTA1753.6343408E-916.3421637
TATCAAC68850.016.0730192
GTACTAA3250.015.5754881
ATACCGT11050.015.5287526
CTAGCGG8800.015.24955729
TACGAAT9300.015.14077341
ATACGAA9450.015.13326240
CGTCGTA9950.015.034739510
AATACGA9500.014.82201839
TACCGTC10900.014.7333197
ACCGTCG10400.014.5955318
CAATACG9500.014.59022738
TCTAGCG9250.014.50788128
Produced by FastQC (version 0.11.5)