##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3713491
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307223041606942	32.0	32.0	32.0	32.0	32.0
2	31.4519660879749	32.0	32.0	32.0	32.0	32.0
3	31.569603103925658	32.0	32.0	32.0	32.0	32.0
4	31.643834601995803	32.0	32.0	32.0	32.0	32.0
5	31.6148914862053	32.0	32.0	32.0	32.0	32.0
6	35.25888119831178	36.0	36.0	36.0	36.0	36.0
7	35.26215386007399	36.0	36.0	36.0	36.0	36.0
8	35.21371615011319	36.0	36.0	36.0	36.0	36.0
9	35.29916404806151	36.0	36.0	36.0	36.0	36.0
10	35.176600401078126	36.0	36.0	36.0	36.0	36.0
11	35.29800960874821	36.0	36.0	36.0	36.0	36.0
12	35.22199811444272	36.0	36.0	36.0	36.0	36.0
13	35.256223321936154	36.0	36.0	36.0	36.0	36.0
14	35.2194624949946	36.0	36.0	36.0	36.0	36.0
15	35.19775327313302	36.0	36.0	36.0	36.0	36.0
16	35.207610035947305	36.0	36.0	36.0	36.0	36.0
17	35.19244990764755	36.0	36.0	36.0	36.0	36.0
18	35.187771829795736	36.0	36.0	36.0	36.0	36.0
19	35.178705428396086	36.0	36.0	36.0	36.0	36.0
20	35.17951086996037	36.0	36.0	36.0	36.0	36.0
21	35.17252956853807	36.0	36.0	36.0	36.0	36.0
22	35.15990829114706	36.0	36.0	36.0	36.0	36.0
23	35.12791333007135	36.0	36.0	36.0	36.0	36.0
24	35.11176895271861	36.0	36.0	36.0	36.0	36.0
25	35.0969995618678	36.0	36.0	36.0	36.0	36.0
26	35.05599044133943	36.0	36.0	36.0	36.0	36.0
27	35.04283866582685	36.0	36.0	36.0	36.0	36.0
28	35.02615517312416	36.0	36.0	36.0	36.0	36.0
29	35.00515175612382	36.0	36.0	36.0	36.0	36.0
30	34.98795553833307	36.0	36.0	36.0	36.0	36.0
31	34.993559698946356	36.0	36.0	36.0	36.0	36.0
32	34.969740063999076	36.0	36.0	36.0	36.0	36.0
33	34.94419940697311	36.0	36.0	36.0	36.0	36.0
34	34.935301849391855	36.0	36.0	36.0	36.0	36.0
35	34.92653301165938	36.0	36.0	36.0	36.0	36.0
36	34.905032219009016	36.0	36.0	36.0	32.0	36.0
37	34.89686470224379	36.0	36.0	36.0	32.0	36.0
38	34.877478362004915	36.0	36.0	36.0	32.0	36.0
39	34.865106176371505	36.0	36.0	36.0	32.0	36.0
40	34.85005484057993	36.0	36.0	36.0	32.0	36.0
41	34.82727735168875	36.0	36.0	36.0	32.0	36.0
42	34.800021327640216	36.0	36.0	36.0	32.0	36.0
43	34.79229490525223	36.0	36.0	36.0	32.0	36.0
44	34.75239498358822	36.0	36.0	36.0	32.0	36.0
45	34.736117577772504	36.0	36.0	36.0	32.0	36.0
46	34.729731134396175	36.0	36.0	36.0	32.0	36.0
47	34.702841342553405	36.0	36.0	36.0	32.0	36.0
48	34.67942967951181	36.0	36.0	36.0	32.0	36.0
49	34.67062852717295	36.0	36.0	36.0	32.0	36.0
50	34.25459843581148	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	14.0
20	48.0
21	118.0
22	261.0
23	693.0
24	1812.0
25	4147.0
26	8716.0
27	16281.0
28	28695.0
29	45185.0
30	67880.0
31	100807.0
32	152652.0
33	259622.0
34	599460.0
35	2427094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.901914065709384	17.713707705963085	12.066062272123009	26.31831595620452
2	16.4166712672032	19.719659012720374	36.77626715607399	27.08740256400244
3	18.8096458230723	23.07351346431369	28.18324467891734	29.93359603369667
4	13.045386696984965	15.80424770344008	34.58911551912501	36.561250080449945
5	15.129006710400919	36.16544979667369	32.637393028496916	16.068150464428467
6	35.47083130805699	34.2053084905419	16.25987448448021	14.063985716920898
7	31.222884342522978	29.7479379915018	19.901731281966214	19.12744638400901
8	28.502640776563076	32.917812376548106	18.65341803709771	19.92612880979111
9	27.202920378694873	14.52888939275738	18.351518826893614	39.91667140165413
10	16.87357462137339	26.482387039459137	30.308332841881498	26.33570549728597
11	37.53586584698873	21.07790216806773	21.59803268676294	19.788199298180604
12	24.765052614911415	23.320697424606657	27.99505909668288	23.91919086379905
13	29.574570128216283	19.280348329913817	24.820472164871276	26.324609376998627
14	23.969251574865808	19.108219193206608	24.593677485686648	32.328851746240936
15	25.522264629158926	26.635879823055987	21.724167367040877	26.11768818074421
16	26.21224761503649	25.55940879079805	23.054153603876046	25.174189990289413
17	24.532279733544527	25.83076140483443	24.528401980777655	25.10855688084339
18	25.362052088536778	24.924807828764624	25.22961584603142	24.483524236667172
19	26.00870717069195	24.813551453335958	24.758778195503908	24.41896318046819
20	26.22700910547274	24.140987930192722	24.34198532320483	25.290017641129708
21	26.936271018295184	24.026152210951903	24.197608126692646	24.839968644060264
22	26.36737237278884	23.98473565709463	24.576146811719752	25.071745158396773
23	25.012784473038696	24.03424380683503	25.103803996726526	25.849167723399745
24	25.211561739673698	24.547570927882525	24.837166466269284	25.403700866174496
25	25.366139236586292	24.12183403267772	24.834885247738104	25.67714148299788
26	25.117585312297862	24.94507838212951	25.046492852784212	24.890843452788413
27	25.602986096318897	24.45796755978292	24.51855767519228	25.420488668705904
28	25.309855623773537	24.317132737099975	24.943212771612608	25.42979886751388
29	25.11596957458164	24.672287997707805	25.021852852611865	25.18988957509868
30	25.038380823402974	24.714665069586363	25.164570992472484	25.08238311453818
31	25.58193518087655	24.453772641883393	24.407724092170348	25.556568085069713
32	25.490518698563903	24.46996297530044	24.33976119890119	25.69975712723447
33	25.036286698037046	24.307025050882164	24.82710297235856	25.829585278722234
34	25.684456743260185	24.36808191614496	24.838560090654095	25.108901249940757
35	25.997243560363497	24.16882681124959	24.94890245984501	24.885027168541903
36	25.023623374605457	24.804018222245798	24.568255731882978	25.60410267126576
37	25.828922770675337	24.514667555691037	24.627393404595317	25.02901626903831
38	25.20749343407591	24.34232370564517	24.914022950372036	25.536159909906875
39	25.82555038936712	24.3412750689249	24.325952440068054	25.507222101639925
40	25.858821254716922	24.541704827075115	24.611396661524157	24.988077256683805
41	24.99473535391617	24.92902826212821	25.00119834655105	25.075038037404568
42	26.071895521644468	24.740224678554547	24.481344785844417	24.70653501395657
43	25.69156293222683	24.103661190177654	24.726983449886465	25.47779242770905
44	25.289598944342334	24.626581298431997	24.519184289013214	25.564635468212447
45	25.40070323522133	24.845552967637015	24.49611664107024	25.257627156071415
46	25.356996764967178	24.604749975022948	24.590261939904813	25.447991320105057
47	25.326650243493116	24.551468552710524	24.94269482950096	25.179186374295398
48	25.947186615603908	25.09730460914018	23.994604317031115	24.960904458224796
49	25.34601589242679	25.096186964784543	24.15477059607507	25.40302654671359
50	25.538450103892995	25.08466552271266	24.27299137510381	25.103892998290533
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	106.0
1	77.0
2	48.0
3	167.0
4	286.0
5	324.5
6	363.0
7	335.5
8	308.0
9	422.0
10	536.0
11	823.0
12	1110.0
13	2053.0
14	2996.0
15	3976.5
16	4957.0
17	5071.5
18	5186.0
19	5303.5
20	5421.0
21	6507.5
22	7594.0
23	9419.5
24	11245.0
25	14439.5
26	17634.0
27	22162.0
28	26690.0
29	31527.5
30	36365.0
31	42894.0
32	49423.0
33	57434.0
34	65445.0
35	76767.5
36	88090.0
37	104509.5
38	120929.0
39	135712.5
40	150496.0
41	166485.0
42	182474.0
43	193693.5
44	204913.0
45	223785.5
46	242658.0
47	258992.0
48	275326.0
49	286720.5
50	298115.0
51	292409.0
52	286703.0
53	285428.5
54	284154.0
55	285390.0
56	286626.0
57	277317.0
58	268008.0
59	245519.5
60	223031.0
61	197180.5
62	171330.0
63	148192.5
64	125055.0
65	105491.0
66	85927.0
67	74033.0
68	62139.0
69	54630.0
70	47121.0
71	36998.5
72	26876.0
73	23329.0
74	19782.0
75	14714.0
76	9646.0
77	8331.0
78	7016.0
79	6002.5
80	4989.0
81	3827.0
82	2665.0
83	2279.0
84	1893.0
85	1471.5
86	1050.0
87	717.0
88	384.0
89	254.0
90	124.0
91	98.5
92	73.0
93	60.5
94	48.0
95	59.0
96	70.0
97	57.0
98	44.0
99	33.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03328404458230813
2	0.0042008988307767545
3	2.154307092706028E-4
4	5.38576773176507E-5
5	5.38576773176507E-5
6	1.615730319529521E-4
7	0.0
8	0.0
9	0.0
10	1.077153546353014E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.5779025720003093E-4
17	0.0
18	1.615730319529521E-4
19	0.0
20	2.692883865882535E-4
21	0.0
22	0.0
23	5.38576773176507E-5
24	9.694381917177125E-4
25	3.500749025647295E-4
26	4.5779025720003093E-4
27	3.7700374122355486E-4
28	0.0018311610288001237
29	4.5779025720003093E-4
30	0.0012925842556236167
31	5.116479345176816E-4
32	6.462921278118084E-4
33	2.4235954792942814E-4
34	9.425093530588872E-4
35	1.3464419329412675E-4
36	2.154307092706028E-4
37	0.0015349438035530446
38	0.0
39	7.80936321105935E-4
40	0.0
41	0.0011040823850118392
42	0.0050087639905415145
43	0.004389400701388532
44	0.0044701872173650075
45	0.0022081647700236784
46	0.0022081647700236784
47	0.0010502247076941886
48	0.004093183476141453
49	0.004039325798823802
50	0.001481086126235394
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3713491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.27294072196072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.05569890483275	31.49137321214933
2	16.85899388107429	15.602304489816984
3	6.520262582042394	9.051351718513988
4	3.0034089369608763	5.559062548151908
5	1.5887565733394247	3.675821936988032
6	0.9538282023428487	2.6481861519566996
7	0.6218955715863727	2.014385584348627
8	0.42510742520804334	1.5736776549693712
9	0.315204113183868	1.312687912020779
>10	1.498305118200762	12.107741662617439
>50	0.08569891485623905	2.7705205416550154
>100	0.05909649226423576	5.550132358446571
>500	0.008772309024899732	2.8111891337036723
>1k	0.004970975082885986	3.8315650946615127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3843	0.1034875269658658	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3836	0.10329902509525403	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.078651597647605E-5
2	0.0	0.0	0.0	0.0	8.078651597647605E-5
3	0.0	0.0	0.0	0.0	8.078651597647605E-5
4	0.0	0.0	0.0	2.692883865882535E-5	8.078651597647605E-5
5	0.0	0.0	0.0	2.692883865882535E-5	1.077153546353014E-4
6	0.0	0.0	0.0	2.692883865882535E-5	1.8850187061177743E-4
7	0.0	0.0	0.0	2.692883865882535E-5	1.8850187061177743E-4
8	0.0	0.0	0.0	2.692883865882535E-5	1.8850187061177743E-4
9	0.0	0.0	0.0	2.692883865882535E-5	1.8850187061177743E-4
10	0.0	0.0	0.0	2.692883865882535E-5	2.4235954792942814E-4
11	0.0	0.0	0.0	2.692883865882535E-5	2.692883865882535E-4
12	0.0	0.0	0.0	2.692883865882535E-5	6.462921278118084E-4
13	0.0	0.0	0.0	2.692883865882535E-5	7.540074824471097E-4
14	0.0	0.0	0.0	2.692883865882535E-5	7.540074824471097E-4
15	0.0	0.0	0.0	5.38576773176507E-5	8.617228370824112E-4
16	0.0	0.0	0.0	5.38576773176507E-5	9.694381917177125E-4
17	0.0	0.0	0.0	5.38576773176507E-5	9.694381917177125E-4
18	0.0	0.0	0.0	5.38576773176507E-5	0.001077153546353014
19	0.0	0.0	0.0	5.38576773176507E-5	0.001077153546353014
20	0.0	0.0	0.0	5.38576773176507E-5	0.0012117977396471408
21	0.0	0.0	0.0	5.38576773176507E-5	0.0012117977396471408
22	0.0	2.692883865882535E-5	0.0	8.078651597647605E-5	0.0012925842556236167
23	0.0	2.692883865882535E-5	0.0	1.077153546353014E-4	0.0012925842556236167
24	0.0	2.692883865882535E-5	0.0	1.077153546353014E-4	0.0012925842556236167
25	0.0	2.692883865882535E-5	0.0	1.615730319529521E-4	0.0012925842556236167
26	0.0	2.692883865882535E-5	0.0	1.8850187061177743E-4	0.0015080149648942194
27	0.0	2.692883865882535E-5	0.0	1.8850187061177743E-4	0.0015349438035530448
28	0.0	2.692883865882535E-5	0.0	3.231460639059042E-4	0.0015349438035530448
29	0.0	2.692883865882535E-5	0.0	5.116479345176816E-4	0.0015349438035530448
30	0.0	2.692883865882535E-5	0.0	8.886516757412365E-4	0.0015349438035530448
31	0.0	2.692883865882535E-5	0.0	0.002073520576729552	0.0015618726422118702
32	0.0	2.692883865882535E-5	0.0	0.003500749025647295	0.0015888014808706956
33	0.0	2.692883865882535E-5	0.0	0.004847190958588563	0.0016157303195295208
34	0.0	2.692883865882535E-5	0.0	0.006489850116776909	0.001696516835505997
35	0.0	2.692883865882535E-5	0.0	0.008482584177529985	0.0017234456741648224
36	0.0	2.692883865882535E-5	0.0	0.011687115977930202	0.0017234456741648224
37	0.0	2.692883865882535E-5	0.0	0.016776666484448192	0.0017234456741648224
38	0.0	2.692883865882535E-5	0.0	0.024289812470260463	0.0017503745128236475
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4140	0.0	26.740211	1
GGTATCA	2040	0.0	25.24544	1
CGCAATA	800	0.0	17.324963	36
TCAACGC	6390	0.0	16.972673	4
ATCAACG	6520	0.0	16.735485	3
CAACGCA	6575	0.0	16.528574	5
AACGCAG	6655	0.0	16.495165	6
TACCGTA	175	3.6343408E-9	16.342163	7
TATCAAC	6885	0.0	16.073019	2
GTACTAA	325	0.0	15.575488	1
ATACCGT	1105	0.0	15.528752	6
CTAGCGG	880	0.0	15.249557	29
TACGAAT	930	0.0	15.140773	41
ATACGAA	945	0.0	15.133262	40
CGTCGTA	995	0.0	15.0347395	10
AATACGA	950	0.0	14.822018	39
TACCGTC	1090	0.0	14.733319	7
ACCGTCG	1040	0.0	14.595531	8
CAATACG	950	0.0	14.590227	38
TCTAGCG	925	0.0	14.507881	28
>>END_MODULE