FastQCFastQC Report
Thu 2 Feb 2017
SRR4062198_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062198_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4286421
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69510.16216325927854497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.014.4863641
GTATCAA45550.013.0703211
CAAGACG7850.012.1023794
CGGACCA8150.011.4252249
TAGATCG1251.8317223E-811.4013755
GCGTTAT3800.011.2443911
CGTTATT3550.011.2363262
AAGACGG8950.010.93427855
ACCGCGT803.768572E-410.6890398
CGCAAGA9250.010.6781252
GCGCAAG9800.010.5610591
GTCTTAC1909.094947E-1210.4947651
ACGAACG2100.010.40492215
GACGGAC9050.010.3941257
GGTTCTA8800.010.36428113
CGGACAT5050.010.3477825
CGGTTCT8850.010.30608712
AACCGCG2053.6379788E-1210.1969467
CTTATAC3000.010.1321873
GCCGGTC5100.010.0602718
Produced by FastQC (version 0.11.5)