##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062198_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4286421
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243365502361993	32.0	32.0	32.0	32.0	32.0
2	30.699967175412773	32.0	32.0	32.0	32.0	32.0
3	30.73207648058835	32.0	32.0	32.0	32.0	32.0
4	30.745529195568984	32.0	32.0	32.0	32.0	32.0
5	30.627178011679206	32.0	32.0	32.0	32.0	32.0
6	34.316801592750686	36.0	36.0	36.0	32.0	36.0
7	34.242701311887004	36.0	36.0	36.0	32.0	36.0
8	34.18693870714053	36.0	36.0	36.0	32.0	36.0
9	34.35766902037854	36.0	36.0	36.0	32.0	36.0
10	33.976502774692456	36.0	36.0	36.0	32.0	36.0
11	34.34883367732661	36.0	36.0	36.0	32.0	36.0
12	34.12597386024378	36.0	36.0	36.0	32.0	36.0
13	34.23958169297883	36.0	36.0	36.0	32.0	36.0
14	34.12582058551878	36.0	36.0	36.0	32.0	36.0
15	34.06060253997449	36.0	36.0	36.0	32.0	36.0
16	34.07204985231269	36.0	36.0	36.0	32.0	36.0
17	33.996613725063405	36.0	36.0	36.0	32.0	36.0
18	34.006025773016695	36.0	36.0	36.0	32.0	36.0
19	34.01411480580186	36.0	36.0	36.0	32.0	36.0
20	34.00215587782908	36.0	36.0	36.0	32.0	36.0
21	33.99173949549053	36.0	36.0	36.0	32.0	36.0
22	33.956535533957116	36.0	36.0	36.0	32.0	36.0
23	33.9189330679371	36.0	36.0	36.0	32.0	36.0
24	33.89279191194705	36.0	36.0	36.0	32.0	36.0
25	33.52599989595049	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	75.0
5	348.0
6	944.0
7	208.0
8	749.0
9	563.0
10	319.0
11	90.0
12	188.0
13	214.0
14	590.0
15	895.0
16	1421.0
17	1856.0
18	2653.0
19	3718.0
20	5875.0
21	8773.0
22	13687.0
23	20174.0
24	28651.0
25	39994.0
26	54625.0
27	69871.0
28	91687.0
29	119776.0
30	155231.0
31	210094.0
32	294410.0
33	414301.0
34	887081.0
35	1857354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73638166178336	16.86659880817596	11.665905727712325	25.73111380232836
2	17.039771949179887	18.884671169110394	37.07129947580879	27.004257405900923
3	19.630816938197075	22.217193377260035	27.814313373303317	30.33767631123957
4	13.267829548612317	15.026011669261575	34.435894307017726	37.270264475108384
5	15.494523343992434	35.820298843804096	32.886377649586436	15.798800162617033
6	35.7194132163358	33.927985726101475	16.164307643319688	14.188293414243036
7	31.5307770169749	29.681222607476315	19.41853464040503	19.369465735143756
8	28.672260766516867	32.929774334871524	18.457142963910673	19.940821934700935
9	26.996429075287466	14.7536790708495	18.27111465732857	39.978777196534466
10	16.69485991952016	26.50982636678484	30.46235610870035	26.332957604994654
11	37.369413114806186	21.27548774709763	21.48701537184846	19.868083766247725
12	24.809179475834306	23.306981065473874	27.874479073343345	24.00936038534848
13	29.50422250420008	19.31713912492595	24.84159769318027	26.3370406776937
14	23.661650229644938	19.233183973810387	24.598845422236508	32.50632037430817
15	25.474940703225986	26.798614302197336	21.648783215946786	26.077661778629896
16	26.103835221400047	25.862920727359022	22.962159793533896	25.07108425770703
17	24.44979522293522	26.01415044205824	24.470397900302196	25.065656434704348
18	25.16729420727969	25.122427058184122	25.184437615627502	24.52584111890869
19	25.810059988529133	24.953434628196288	24.894398891024885	24.34210649224969
20	26.012770522435986	24.359220448901798	24.493235275299003	25.134773753363216
21	26.542488713554174	24.191185725404445	24.30268994890421	24.963635612137168
22	26.212185542192312	24.0505486590747	24.571182752305404	25.166083046427584
23	24.841931938562723	24.266047944724104	25.081120863022775	25.810899253690394
24	24.845822796042416	24.734506971541727	24.927902746350767	25.491767486065093
25	25.180202678831414	24.30866362221103	24.83106400198574	25.68006969697182
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	373.0
1	373.0
2	805.5
3	1238.0
4	1238.0
5	1238.0
6	2625.0
7	4012.0
8	4012.0
9	4012.0
10	4949.5
11	5887.0
12	5887.0
13	5887.0
14	7972.0
15	10057.0
16	10057.0
17	10057.0
18	16790.5
19	23524.0
20	23524.0
21	23524.0
22	36876.0
23	50228.0
24	50228.0
25	50228.0
26	74319.5
27	98411.0
28	98411.0
29	98411.0
30	126162.5
31	153914.0
32	153914.0
33	153914.0
34	194880.5
35	235847.0
36	235847.0
37	235847.0
38	283447.5
39	331048.0
40	331048.0
41	331048.0
42	385465.0
43	439882.0
44	439882.0
45	439882.0
46	495517.5
47	551153.0
48	551153.0
49	551153.0
50	573616.5
51	596080.0
52	596080.0
53	596080.0
54	568111.5
55	540143.0
56	540143.0
57	540143.0
58	498101.5
59	456060.0
60	456060.0
61	456060.0
62	400828.5
63	345597.0
64	345597.0
65	345597.0
66	281792.0
67	217987.0
68	217987.0
69	217987.0
70	165708.5
71	113430.0
72	113430.0
73	113430.0
74	85405.5
75	57381.0
76	57381.0
77	57381.0
78	44709.0
79	32037.0
80	32037.0
81	32037.0
82	22225.0
83	12413.0
84	12413.0
85	12413.0
86	8979.0
87	5545.0
88	5545.0
89	5545.0
90	3738.5
91	1932.0
92	1932.0
93	1932.0
94	1218.0
95	504.0
96	504.0
97	504.0
98	1121.0
99	1738.0
100	1738.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011664743150521146
2	1.8663589040833833E-4
3	8.865204794396071E-4
4	0.010731563698479454
5	0.03515753585567073
6	0.06527590267031633
7	0.10934530229298521
8	0.1112116611970686
9	0.12077675058049593
10	0.13638417691589322
11	0.14298642153908822
12	0.1359642461624745
13	0.1294553194844837
14	0.11732398660794169
15	0.13610422308028072
16	0.12210653129965535
17	0.12653913369685338
18	0.1141278469846989
19	0.09959357701914956
20	0.09469438489593066
21	0.09499766821784422
22	0.10561258448481844
23	0.08431276349196684
24	0.09357456955348063
25	0.09056506582064618
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4286421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.587525863120156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.40192970027435	33.73026772451338
2	14.98607659056092	13.96328461498913
3	5.147755522043351	7.194635805606427
4	2.340350092713487	4.361244818921809
5	1.2717712922493294	2.9624338984819687
6	0.7895418356848906	2.206968041399112
7	0.519233721721096	1.6932870107779985
8	0.40356218737467614	1.5040771073356052
9	0.28336977373122263	1.1881346996276858
>10	1.650863626554877	14.124214916386531
>50	0.12052396315747524	3.84922038734554
>100	0.07233188963714758	6.711577663466456
>500	0.007654167826406185	2.4834059567826676
>1k	0.00498528010615471	3.8641783927764393
>5k	5.035636470863272E-5	0.16306896158934395
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6951	0.16216325927854497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.998845890312688E-5
2	0.0	0.0	0.0	0.0	6.998845890312688E-5
3	0.0	0.0	0.0	0.0	6.998845890312688E-5
4	0.0	0.0	0.0	2.332948630104229E-5	6.998845890312688E-5
5	0.0	0.0	0.0	2.332948630104229E-5	9.331794520416917E-5
6	0.0	0.0	0.0	2.332948630104229E-5	1.3997691780625376E-4
7	0.0	0.0	0.0	2.332948630104229E-5	1.3997691780625376E-4
8	0.0	0.0	0.0	2.332948630104229E-5	1.3997691780625376E-4
9	0.0	0.0	0.0	2.332948630104229E-5	1.3997691780625376E-4
10	0.0	0.0	0.0	2.332948630104229E-5	1.3997691780625376E-4
11	0.0	0.0	0.0	2.332948630104229E-5	1.3997691780625376E-4
12	0.0	0.0	0.0	6.998845890312688E-5	3.499422945156344E-4
13	0.0	0.0	0.0	6.998845890312688E-5	4.665897260208458E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1560	0.0	14.486364	1
GTATCAA	4555	0.0	13.070321	1
CAAGACG	785	0.0	12.102379	4
CGGACCA	815	0.0	11.425224	9
TAGATCG	125	1.8317223E-8	11.401375	5
GCGTTAT	380	0.0	11.244391	1
CGTTATT	355	0.0	11.236326	2
AAGACGG	895	0.0	10.9342785	5
ACCGCGT	80	3.768572E-4	10.689039	8
CGCAAGA	925	0.0	10.678125	2
GCGCAAG	980	0.0	10.561059	1
GTCTTAC	190	9.094947E-12	10.494765	1
ACGAACG	210	0.0	10.404922	15
GACGGAC	905	0.0	10.394125	7
GGTTCTA	880	0.0	10.364281	13
CGGACAT	505	0.0	10.347782	5
CGGTTCT	885	0.0	10.306087	12
AACCGCG	205	3.6379788E-12	10.196946	7
CTTATAC	300	0.0	10.132187	3
GCCGGTC	510	0.0	10.060271	8
>>END_MODULE