FastQCFastQC Report
Thu 2 Feb 2017
SRR4062198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4286421
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49200.027.6849021
GGTATCA22650.026.6194951
CGCAATA9150.019.23490536
TCTAGCG9450.017.92607328
TCAACGC75750.017.7445324
TATCAAC76450.017.7265572
ATCAACG76500.017.5130523
ATACGAA9900.017.33428640
CAACGCA77800.017.3052485
CTAGCGG9650.017.09858529
AACGCAG78900.017.0918646
CGAATGC10500.016.34394643
CAATACG10500.016.34356338
TACGAAT10600.016.18975641
TAGCGGC10250.015.88287330
CGGTCCA11950.015.64793310
CCGCGTA850.0029823615.5288159
GCGCAAT10650.015.49290535
GGCGTTA10900.015.13862142
AATACGA11350.015.11977339
Produced by FastQC (version 0.11.5)