##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062197_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3619
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.570875932578062	32.0	32.0	32.0	14.0	32.0
2	26.73970710140923	32.0	14.0	32.0	14.0	32.0
3	27.138988670903565	32.0	21.0	32.0	14.0	32.0
4	26.634705719812104	32.0	21.0	32.0	14.0	32.0
5	26.942525559546837	32.0	21.0	32.0	14.0	32.0
6	30.120198949986182	36.0	21.0	36.0	14.0	36.0
7	29.72257529704338	36.0	21.0	36.0	14.0	36.0
8	29.622547665100857	36.0	21.0	36.0	14.0	36.0
9	30.058579718154185	36.0	21.0	36.0	14.0	36.0
10	28.84885327438519	36.0	21.0	36.0	14.0	36.0
11	30.415584415584416	36.0	21.0	36.0	14.0	36.0
12	29.587455098093397	36.0	21.0	36.0	14.0	36.0
13	29.982868195634154	36.0	21.0	36.0	14.0	36.0
14	29.569494335451783	36.0	21.0	36.0	14.0	36.0
15	29.29483282674772	36.0	21.0	36.0	14.0	36.0
16	29.529704338214977	36.0	21.0	36.0	14.0	36.0
17	29.34871511467256	36.0	21.0	36.0	14.0	36.0
18	29.322188449848024	36.0	21.0	36.0	14.0	36.0
19	29.22160817905499	36.0	21.0	36.0	14.0	36.0
20	29.51616468637745	36.0	21.0	36.0	14.0	36.0
21	29.051671732522795	36.0	21.0	36.0	14.0	36.0
22	29.061619231831997	36.0	21.0	36.0	14.0	36.0
23	28.90909090909091	36.0	21.0	36.0	14.0	36.0
24	29.02873722022658	36.0	21.0	36.0	14.0	36.0
25	27.994749930920143	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	15.0
16	22.0
17	40.0
18	66.0
19	95.0
20	122.0
21	117.0
22	107.0
23	140.0
24	150.0
25	156.0
26	162.0
27	185.0
28	180.0
29	203.0
30	226.0
31	238.0
32	301.0
33	339.0
34	413.0
35	335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06106659298149	19.176568112738327	11.356728378004973	28.405636916275213
2	18.20945012434374	18.596297319701574	36.99917104172423	26.195081514230452
3	18.95551257253385	20.641061066592982	27.521414755457307	32.88201160541586
4	12.572533849129593	15.556783641890023	32.246476927328	39.62420558165239
5	15.998894722298978	34.37413650179607	33.57281016855485	16.054158607350097
6	36.39126830616192	34.18071290411716	15.142304504006631	14.285714285714285
7	30.671456203371093	29.31749101961868	18.87261674495717	21.138436032053054
8	27.41088698535507	33.73860182370821	18.26471400939486	20.585797181541864
9	26.167449571704893	13.760707377728654	18.623929262227136	41.44791378833932
10	17.297596021000274	24.785852445426915	31.914893617021278	26.001657916551537
11	39.071566731141196	20.17131804365847	19.95026250345399	20.80685272174634
12	23.21083172147002	22.879248411163307	29.289859077093123	24.620060790273556
13	29.372754904669797	18.043658469190387	23.791102514506772	28.792484111633048
14	22.188449848024316	18.706825089803814	25.58717877866814	33.51754628350373
15	25.20033158331031	25.310859353412546	22.768720641061066	26.720088422216083
16	26.029289859077092	24.785852445426915	23.87399834208345	25.310859353412546
17	24.150317767339043	25.36612323846366	25.58717877866814	24.896380215529152
18	24.592428847747996	23.376623376623375	26.167449571704893	25.863498203923736
19	24.067421939762365	24.4542691351202	26.33324122685825	25.145067698259187
20	26.360873169383808	23.514783089251175	24.067421939762365	26.056921801602652
21	24.785852445426915	24.095053882287925	25.670074606244818	25.44901906604034
22	24.924012158054712	23.73583862945565	25.227963525835868	26.11218568665377
23	23.791102514506772	24.592428847747996	24.924012158054712	26.692456479690524
24	23.625310859353412	25.97402597402597	25.421387123514783	24.97927604310583
25	25.06217187068251	22.823984526112184	25.58717877866814	26.526664824537168
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.0
9	2.0
10	4.0
11	6.0
12	6.0
13	6.0
14	9.0
15	12.0
16	12.0
17	12.0
18	24.0
19	36.0
20	36.0
21	36.0
22	43.0
23	50.0
24	50.0
25	50.0
26	78.5
27	107.0
28	107.0
29	107.0
30	111.5
31	116.0
32	116.0
33	116.0
34	168.0
35	220.0
36	220.0
37	220.0
38	242.0
39	264.0
40	264.0
41	264.0
42	309.0
43	354.0
44	354.0
45	354.0
46	391.0
47	428.0
48	428.0
49	428.0
50	444.5
51	461.0
52	461.0
53	461.0
54	441.5
55	422.0
56	422.0
57	422.0
58	418.0
59	414.0
60	414.0
61	414.0
62	345.0
63	276.0
64	276.0
65	276.0
66	234.0
67	192.0
68	192.0
69	192.0
70	150.0
71	108.0
72	108.0
73	108.0
74	83.5
75	59.0
76	59.0
77	59.0
78	54.5
79	50.0
80	50.0
81	50.0
82	35.5
83	21.0
84	21.0
85	21.0
86	15.5
87	10.0
88	10.0
89	10.0
90	7.5
91	5.0
92	5.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	3.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3619.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.81044487427465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.52282768777614	89.6103896103896
2	3.122238586156112	5.857971815418623
3	0.9131075110456555	2.5697706548770376
4	0.29455081001472755	1.1052777010223818
5	0.029455081001472753	0.13815971262779772
6	0.05891016200294551	0.33158331030671456
7	0.05891016200294551	0.3868471953578337
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCTGGTGGTGCCCTTCCGTCAAT	7	0.19342359767891684	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7	0.19342359767891684	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	6	0.16579165515335728	No Hit
GGCGGTGACTCGCCTCGCGGCGGAC	6	0.16579165515335728	No Hit
TTCGTATTGCGCCGCTAGAGGTGAA	5	0.13815971262779772	No Hit
GGCCCGAGCCGCCTGGATACCGCAG	4	0.11052777010223819	No Hit
CCCTTCACCTGACCAATGACTTCCA	4	0.11052777010223819	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	4	0.11052777010223819	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4	0.11052777010223819	No Hit
GAATAGGACCGCGGTTCTATTTTGT	4	0.11052777010223819	No Hit
GAATAACGCCGCCGCATCGCCAGTC	4	0.11052777010223819	No Hit
TGTCAATCCTGTCCGTGTCCGGGCC	4	0.11052777010223819	No Hit
TCCCGGCTCCGGCCGGGGGTCGGGC	4	0.11052777010223819	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	4	0.11052777010223819	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	4	0.11052777010223819	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE