##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062197_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3619
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.169660127106937	32.0	32.0	32.0	21.0	32.0
2	28.877866814037027	32.0	32.0	32.0	14.0	32.0
3	30.464216634429402	32.0	32.0	32.0	21.0	32.0
4	30.97043382149765	32.0	32.0	32.0	32.0	32.0
5	30.586902459242886	32.0	32.0	32.0	32.0	32.0
6	34.06714562033711	36.0	36.0	36.0	32.0	36.0
7	34.15114672561481	36.0	36.0	36.0	32.0	36.0
8	33.882287924841116	36.0	36.0	36.0	32.0	36.0
9	34.362254766510084	36.0	36.0	36.0	32.0	36.0
10	33.93727549046698	36.0	36.0	36.0	32.0	36.0
11	34.560928433268856	36.0	36.0	36.0	32.0	36.0
12	34.360596849958554	36.0	36.0	36.0	32.0	36.0
13	34.5407571152252	36.0	36.0	36.0	32.0	36.0
14	34.21027908261951	36.0	36.0	36.0	32.0	36.0
15	34.20033158331031	36.0	36.0	36.0	32.0	36.0
16	34.20613429124067	36.0	36.0	36.0	32.0	36.0
17	34.05802707930368	36.0	36.0	36.0	32.0	36.0
18	34.21635810997513	36.0	36.0	36.0	32.0	36.0
19	34.32135949157226	36.0	36.0	36.0	32.0	36.0
20	34.27079303675048	36.0	36.0	36.0	32.0	36.0
21	34.269964078474715	36.0	36.0	36.0	32.0	36.0
22	34.209173804918485	36.0	36.0	36.0	32.0	36.0
23	34.107764575849686	36.0	36.0	36.0	32.0	36.0
24	34.02846090080133	36.0	36.0	36.0	32.0	36.0
25	33.933130699088146	36.0	36.0	36.0	32.0	36.0
26	33.8543796628903	36.0	36.0	36.0	32.0	36.0
27	33.98590770931197	36.0	36.0	36.0	32.0	36.0
28	33.8469190384084	36.0	36.0	36.0	32.0	36.0
29	33.70185134014921	36.0	36.0	36.0	27.0	36.0
30	33.88919591047251	36.0	36.0	36.0	32.0	36.0
31	33.71649626968776	36.0	36.0	36.0	27.0	36.0
32	33.719812102790826	36.0	36.0	36.0	32.0	36.0
33	33.67670627245095	36.0	36.0	36.0	32.0	36.0
34	33.60569218016027	36.0	36.0	36.0	27.0	36.0
35	33.76982591876209	36.0	36.0	36.0	32.0	36.0
36	33.8170765404808	36.0	36.0	36.0	32.0	36.0
37	33.47471677258911	36.0	36.0	36.0	21.0	36.0
38	33.51892788063001	36.0	36.0	36.0	21.0	36.0
39	33.54545454545455	36.0	36.0	36.0	27.0	36.0
40	33.43354517822603	36.0	36.0	36.0	21.0	36.0
41	33.42000552638851	36.0	36.0	36.0	21.0	36.0
42	33.00690798563139	36.0	36.0	36.0	14.0	36.0
43	33.59795523625311	36.0	36.0	36.0	27.0	36.0
44	33.39043934788616	36.0	36.0	36.0	21.0	36.0
45	33.19231831997789	36.0	36.0	36.0	21.0	36.0
46	33.07322464769273	36.0	36.0	36.0	14.0	36.0
47	33.25006907985632	36.0	36.0	36.0	21.0	36.0
48	33.07571152252003	36.0	36.0	36.0	14.0	36.0
49	33.025421387123515	36.0	36.0	36.0	14.0	36.0
50	32.44404531638574	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	4.0
24	2.0
25	14.0
26	27.0
27	32.0
28	64.0
29	112.0
30	183.0
31	288.0
32	417.0
33	671.0
34	925.0
35	878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79662890301188	16.44100580270793	12.323846366399557	27.43851892788063
2	19.789939192924265	19.04367053620785	33.41625207296849	27.750138197899393
3	24.150317767339043	19.839734733351754	26.554296767062723	29.455650732246475
4	17.408123791102515	14.783089251174358	33.46228239845261	34.34650455927052
5	21.49765128488533	31.00303951367781	28.405636916275213	19.09367228516165
6	37.3583862945565	32.743851892788065	13.097540757115226	16.800221055540206
7	32.10831721470019	28.599060513954132	17.62917933130699	21.663442940038685
8	29.897761812655432	31.61094224924012	15.943630837247857	22.54766510085659
9	24.924012158054712	13.37386018237082	16.55153357281017	45.1505940867643
10	18.706825089803814	24.758220502901356	26.388505111909367	30.146449295385462
11	38.43603205305333	19.922630560928432	19.09367228516165	22.54766510085659
12	24.758220502901356	21.221331859629732	26.443768996960486	27.57667864050843
13	29.980657640232106	17.656811273832552	22.823984526112184	29.538546559823153
14	24.012158054711247	17.13180436584692	21.96739430781984	36.888643271621994
15	26.13981762917933	23.901630284609006	20.254213871235148	29.70433821497651
16	27.880630008289582	24.39900525006908	21.110804089527495	26.609560652113846
17	25.780602376347055	24.28847747996684	22.326609560652113	27.604310583033985
18	27.466150870406192	23.901630284609006	23.404255319148938	25.227963525835868
19	27.27272727272727	22.07792207792208	23.65294280187897	26.996407847471676
20	26.25034539928157	23.956894169660128	22.962144238739985	26.830616192318317
21	27.217463387676155	22.54766510085659	23.625310859353412	26.609560652113846
22	26.084553744128215	21.24896380215529	23.92926222713457	28.73722022658193
23	25.36612323846366	22.796352583586625	23.791102514506772	28.04642166344294
24	25.11743575573363	24.39900525006908	23.1555678364189	27.327991157778392
25	25.227963525835868	22.934512296214425	22.57529704338215	29.26222713456756
26	25.64244266371926	25.172699640784746	23.18319977894446	26.001657916551537
27	27.659574468085108	22.879248411163307	24.039789997236806	25.421387123514783
28	25.58717877866814	23.570046974302294	24.675324675324674	26.167449571704893
29	26.554296767062723	23.293727549046697	22.906880353688862	27.245095330201714
30	26.25034539928157	24.095053882287925	23.625310859353412	26.029289859077092
31	26.74772036474164	23.597678916827853	23.68057474440453	25.97402597402597
32	24.81348438795247	24.37137330754352	22.962144238739985	27.852998065764023
33	25.64244266371926	24.675324675324674	22.962144238739985	26.720088422216083
34	24.86874827300359	24.039789997236806	23.542415031776734	27.54904669798287
35	25.670074606244818	23.818734457032328	22.796352583586625	27.714838353136223
36	25.918762088974855	24.924012158054712	23.072672008842222	26.084553744128215
37	27.02403978999724	24.012158054711247	23.072672008842222	25.891130146449292
38	25.227963525835868	24.48190107764576	24.122685824813484	26.167449571704893
39	26.222713456756008	24.509533020171318	23.072672008842222	26.195081514230452
40	27.493782812931748	25.227963525835868	22.934512296214425	24.34374136501796
41	26.305609284332686	24.095053882287925	22.685824813484388	26.913512019895
42	24.758220502901356	26.74772036474164	22.74108869853551	25.7529704338215
43	26.056921801602652	22.962144238739985	23.65294280187897	27.327991157778392
44	25.172699640784746	25.283227410886987	23.73583862945565	25.808234318872618
45	25.891130146449292	24.122685824813484	23.072672008842222	26.913512019895
46	25.891130146449292	25.36612323846366	22.07792207792208	26.66482453716496
47	25.7529704338215	24.841116330478034	23.68057474440453	25.72533849129594
48	25.808234318872618	26.13981762917933	23.34899143409782	24.702956617850237
49	25.697706548770377	24.095053882287925	23.1555678364189	27.0516717325228
50	26.41613705443493	24.620060790273556	23.431887261674493	25.53191489361702
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	1.0
10	2.0
11	1.5
12	1.0
13	1.0
14	1.0
15	1.0
16	1.0
17	2.0
18	3.0
19	3.5
20	4.0
21	8.5
22	13.0
23	13.5
24	14.0
25	20.5
26	27.0
27	30.0
28	33.0
29	34.0
30	35.0
31	42.0
32	49.0
33	55.0
34	61.0
35	64.5
36	68.0
37	81.0
38	94.0
39	102.0
40	110.0
41	116.0
42	122.0
43	148.0
44	174.0
45	177.0
46	180.0
47	201.5
48	223.0
49	223.0
50	223.0
51	226.5
52	230.0
53	240.0
54	250.0
55	267.0
56	284.0
57	276.0
58	268.0
59	256.0
60	244.0
61	231.0
62	218.0
63	183.0
64	148.0
65	129.5
66	111.0
67	108.5
68	106.0
69	97.0
70	88.0
71	71.5
72	55.0
73	52.0
74	49.0
75	42.0
76	35.0
77	28.0
78	21.0
79	20.5
80	20.0
81	20.5
82	21.0
83	15.5
84	10.0
85	6.5
86	3.0
87	4.0
88	5.0
89	4.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	3.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.027631942525559547
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3619.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.22492401215806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.95307917888563	90.41171594363084
2	2.932551319648094	5.5263885051119095
3	0.7038123167155426	1.9894998618402875
4	0.17595307917888564	0.6631666206134291
5	0.08797653958944282	0.41447913788339324
6	0.05865102639296188	0.33158331030671456
7	0.02932551319648094	0.19342359767891684
8	0.02932551319648094	0.22105554020447638
9	0.02932551319648094	0.24868748273003594
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	9	0.24868748273003594	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	8	0.22105554020447638	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	7	0.19342359767891684	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	6	0.16579165515335728	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	6	0.16579165515335728	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	5	0.13815971262779772	No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA	5	0.13815971262779772	No Hit
GAGTACGAGCAAATGGTGAAGGAGCTGCGGGAGATCCGCCTGCAGCCGCA	5	0.13815971262779772	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	4	0.11052777010223819	No Hit
GTGAAGGAGCTGCGGGAGATCCGCCTGCAGCCGCAGGAGGCGCCGCTGGC	4	0.11052777010223819	No Hit
CTTTTGTGCCGTCTGGTGCGCTCCCGACGATCCTTGAAAATCCACCGGAA	4	0.11052777010223819	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG	4	0.11052777010223819	No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	4	0.11052777010223819	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC	4	0.11052777010223819	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE