FastQCFastQC Report
Thu 2 Feb 2017
SRR4062196_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062196_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084553
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18380.1694707404801794No Hit
TATCAACGCAGAGTACTTTTTTTTT11870.10944601139824427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAA551.1313454E-515.540131
GGTATCA5000.015.0048591
GTATTGA704.4495937E-714.9234581
CCGTCGT801.2852979E-714.2516979
GCGTTAT801.2925739E-714.2451191
CGTCGTA759.65194E-713.93499210
TTAGACT551.9596113E-413.8179134
GTCGGCG500.001497690813.30158412
ACCGATC604.0877322E-412.667598
TAGGGGC604.090918E-412.666424
CCTATAG604.094106E-412.6652513
CTAGGAC905.407219E-712.6652513
GTATTAG853.955318E-612.28990651
GGTTAAT701.0938048E-412.2101021
CGGTCGG550.00306467812.0923510
ACAACTA550.003065738112.091798
TAGAATC550.003067859212.0906734
ATAGTAC802.8677605E-511.87367253
CGTTATT802.8691156E-511.8731242
GTCCTAC802.8745424E-511.8709331
Produced by FastQC (version 0.11.5)