##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062196_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1084553
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.296288885835917	32.0	32.0	32.0	32.0	32.0
2	30.87194079035326	32.0	32.0	32.0	32.0	32.0
3	30.902523896941872	32.0	32.0	32.0	32.0	32.0
4	30.90838990810039	32.0	32.0	32.0	32.0	32.0
5	30.842277878536134	32.0	32.0	32.0	32.0	32.0
6	34.48952702173153	36.0	36.0	36.0	32.0	36.0
7	34.43954606183377	36.0	36.0	36.0	32.0	36.0
8	34.39736094040586	36.0	36.0	36.0	32.0	36.0
9	34.5337341743557	36.0	36.0	36.0	32.0	36.0
10	34.25251509147086	36.0	36.0	36.0	32.0	36.0
11	34.49571021425417	36.0	36.0	36.0	32.0	36.0
12	34.35616240054659	36.0	36.0	36.0	32.0	36.0
13	34.42544993190743	36.0	36.0	36.0	32.0	36.0
14	34.34323725995871	36.0	36.0	36.0	32.0	36.0
15	34.29435998056342	36.0	36.0	36.0	32.0	36.0
16	34.29602149456965	36.0	36.0	36.0	32.0	36.0
17	34.239853653993855	36.0	36.0	36.0	32.0	36.0
18	34.24578789602721	36.0	36.0	36.0	32.0	36.0
19	34.2476116888709	36.0	36.0	36.0	32.0	36.0
20	34.224733138906075	36.0	36.0	36.0	32.0	36.0
21	34.22554084493796	36.0	36.0	36.0	32.0	36.0
22	34.18848779174462	36.0	36.0	36.0	32.0	36.0
23	34.154361289858585	36.0	36.0	36.0	32.0	36.0
24	34.13309354176329	36.0	36.0	36.0	32.0	36.0
25	33.78553099756305	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	21.0
5	96.0
6	198.0
7	54.0
8	180.0
9	152.0
10	65.0
11	21.0
12	37.0
13	45.0
14	143.0
15	261.0
16	378.0
17	504.0
18	642.0
19	908.0
20	1358.0
21	1860.0
22	2741.0
23	3982.0
24	5717.0
25	8136.0
26	11334.0
27	14788.0
28	19661.0
29	26463.0
30	34964.0
31	48023.0
32	68489.0
33	98348.0
34	221406.0
35	513577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.414704626741404	16.865444708047537	11.760713959704686	25.959136705506374
2	17.124413697465588	18.647255869018608	37.24730326190285	26.981027171612954
3	19.829329350096124	21.782222037813092	27.614621799925317	30.773826812165474
4	13.676661527225514	14.884952749109203	33.4950167093314	37.943369014333875
5	15.694855911661728	35.402270438186456	32.93629565133979	15.966577998812022
6	36.33453241060663	33.299258503407636	15.878101908959314	14.48810717702643
7	31.754800348159655	29.435643536940276	19.179701885987864	19.629854228912205
8	28.693357503752015	33.07246141378489	18.161226784122103	20.072954298340992
9	26.781926236091035	15.06045450265579	18.19746070780416	39.96015855344901
10	17.191661750463695	26.6098141259822	30.132400501129574	26.06612362242453
11	37.178499937214234	21.392034332735022	21.133597033557148	20.2958686964936
12	24.76081093931619	23.23626053535142	27.906205944020112	24.096722581312278
13	29.876974719163364	19.29537884327063	24.566281878005007	26.261364559561002
14	23.81324695632749	18.96294053519158	24.408458127983327	32.8153543804976
15	25.38376955085091	26.626271653539675	21.642506903558832	26.34745189205059
16	26.065323469517743	25.77454153381471	22.830905227243775	25.329229769423772
17	24.5648304740523	25.910218821774357	24.458115163913238	25.066835540260108
18	25.074696851240425	25.20632207196189	24.99439254406814	24.724588532729545
19	25.933100638833157	24.889390127526674	24.593322984519233	24.584186249120936
20	26.124416750339613	24.43742247947552	24.491686846612723	24.946473923572146
21	26.328690108583906	24.157934857039496	24.46847781348227	25.044897220894324
22	26.302814724023772	23.923354447378404	24.576711543626516	25.197119284971308
23	25.19701315143529	24.247658911638446	24.9062457321507	25.649082204775564
24	25.077035381365516	24.49932769960677	24.834878040002103	25.588758879025615
25	25.326754108708556	24.339593563384714	24.732722844779456	25.600929483127278
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	85.0
2	190.5
3	296.0
4	296.0
5	296.0
6	578.5
7	861.0
8	861.0
9	861.0
10	1172.5
11	1484.0
12	1484.0
13	1484.0
14	1983.5
15	2483.0
16	2483.0
17	2483.0
18	4026.5
19	5570.0
20	5570.0
21	5570.0
22	8599.0
23	11628.0
24	11628.0
25	11628.0
26	17050.5
27	22473.0
28	22473.0
29	22473.0
30	29477.5
31	36482.0
32	36482.0
33	36482.0
34	46257.5
35	56033.0
36	56033.0
37	56033.0
38	68662.0
39	81291.0
40	81291.0
41	81291.0
42	94906.0
43	108521.0
44	108521.0
45	108521.0
46	123898.5
47	139276.0
48	139276.0
49	139276.0
50	146069.5
51	152863.0
52	152863.0
53	152863.0
54	148567.5
55	144272.0
56	144272.0
57	144272.0
58	133679.0
59	123086.0
60	123086.0
61	123086.0
62	106588.5
63	90091.0
64	90091.0
65	90091.0
66	72689.5
67	55288.0
68	55288.0
69	55288.0
70	42062.5
71	28837.0
72	28837.0
73	28837.0
74	20982.0
75	13127.0
76	13127.0
77	13127.0
78	9703.0
79	6279.0
80	6279.0
81	6279.0
82	4348.5
83	2418.0
84	2418.0
85	2418.0
86	1693.5
87	969.0
88	969.0
89	969.0
90	644.5
91	320.0
92	320.0
93	320.0
94	206.5
95	93.0
96	93.0
97	93.0
98	260.0
99	427.0
100	427.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.220388491848715E-4
2	1.8440776983697432E-4
3	7.376310793478973E-4
4	0.011894301154484843
5	0.032916786915899915
6	0.06122337958587547
7	0.10529683657691234
8	0.10465140938248291
9	0.11608469111237533
10	0.12908543888588203
11	0.13876684680232315
12	0.13037629327474085
13	0.12548948737406102
14	0.11433281729892407
15	0.13056070104457781
16	0.11682232219172323
17	0.11958843873927784
18	0.10824736089430392
19	0.09358694319226446
20	0.08934556448601405
21	0.08916115671617708
22	0.09884256463261823
23	0.08012517599416534
24	0.08851572952174767
25	0.08667165182337792
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1084553.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.049590347207236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.95207662545684	46.80990268072792
2	12.673401107003585	15.22065089562816
3	3.9124648208771284	7.048257292245933
4	1.7955179117486082	4.312804602463077
5	0.9939290891775079	2.984251731964108
6	0.607647385355911	2.189338593970442
7	0.4173599846765068	1.7543607274998645
8	0.2801960791911247	1.346052781785654
9	0.21751230945052302	1.1755372570180995
>10	1.0693853466475973	11.11010836381323
>50	0.048442642771368824	2.0097148630456134
>100	0.03098753086709304	3.4634723506927347
>500	7.70833411543465E-4	0.29550406408097574
>1k	3.0833336461738597E-4	0.2800437950642079
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1838	0.1694707404801794	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1187	0.10944601139824427	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.220388491848716E-5
7	0.0	0.0	0.0	0.0	9.220388491848716E-5
8	0.0	0.0	0.0	0.0	9.220388491848716E-5
9	0.0	0.0	0.0	0.0	9.220388491848716E-5
10	0.0	0.0	0.0	0.0	9.220388491848716E-5
11	0.0	0.0	0.0	0.0	9.220388491848716E-5
12	0.0	0.0	0.0	0.0	1.8440776983697432E-4
13	0.0	0.0	0.0	9.220388491848716E-5	1.8440776983697432E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAA	55	1.1313454E-5	15.54013	1
GGTATCA	500	0.0	15.004859	1
GTATTGA	70	4.4495937E-7	14.923458	1
CCGTCGT	80	1.2852979E-7	14.251697	9
GCGTTAT	80	1.2925739E-7	14.245119	1
CGTCGTA	75	9.65194E-7	13.934992	10
TTAGACT	55	1.9596113E-4	13.817913	4
GTCGGCG	50	0.0014976908	13.301584	12
ACCGATC	60	4.0877322E-4	12.66759	8
TAGGGGC	60	4.090918E-4	12.66642	4
CCTATAG	60	4.094106E-4	12.665251	3
CTAGGAC	90	5.407219E-7	12.665251	3
GTATTAG	85	3.955318E-6	12.2899065	1
GGTTAAT	70	1.0938048E-4	12.210102	1
CGGTCGG	55	0.003064678	12.09235	10
ACAACTA	55	0.0030657381	12.09179	8
TAGAATC	55	0.0030678592	12.090673	4
ATAGTAC	80	2.8677605E-5	11.8736725	3
CGTTATT	80	2.8691156E-5	11.873124	2
GTCCTAC	80	2.8745424E-5	11.870933	1
>>END_MODULE