FastQCFastQC Report
Thu 2 Feb 2017
SRR4062196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084553
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5900.027.9840161
GTATCAA13000.025.4008771
TCTAGCG1500.021.99988428
CTAGCGG1551.8189894E-1221.29119129
CGCAATA1601.8189894E-1220.62584136
TAGCGGC1653.6379788E-1220.00081830
TCGATCG550.00448225920.00081841
ATACGAA1852.1827873E-1117.83856640
TATCAAC19750.017.154342
TCAACGC19550.016.7664034
TACGAAT2109.094947E-1216.7625941
ATCAACG19650.016.6810783
AATACGA2007.2759576E-1116.50067539
ACACCGT2007.2759576E-1116.4991536
TATACTA1206.4084415E-616.4991535
CAACGCA20100.016.3076215
GCGCAAT2051.05501385E-1016.09821935
AACGCAG20650.015.8732776
GGCGCAA3100.015.61282134
ACCTAAG850.002966984615.5393611
Produced by FastQC (version 0.11.5)