##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062196_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1084553 Sequences flagged as poor quality 0 Sequence length 50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29916841316192 32.0 32.0 32.0 32.0 32.0 2 31.46730311934963 32.0 32.0 32.0 32.0 32.0 3 31.582452863068934 32.0 32.0 32.0 32.0 32.0 4 31.660738571558973 32.0 32.0 32.0 32.0 32.0 5 31.626960600357936 32.0 32.0 32.0 32.0 32.0 6 35.28219644406497 36.0 36.0 36.0 36.0 36.0 7 35.286062552959606 36.0 36.0 36.0 36.0 36.0 8 35.235475813537924 36.0 36.0 36.0 36.0 36.0 9 35.322125336428925 36.0 36.0 36.0 36.0 36.0 10 35.20239766982342 36.0 36.0 36.0 36.0 36.0 11 35.31718044208075 36.0 36.0 36.0 36.0 36.0 12 35.25254644079174 36.0 36.0 36.0 36.0 36.0 13 35.27729488554271 36.0 36.0 36.0 36.0 36.0 14 35.248204559850926 36.0 36.0 36.0 36.0 36.0 15 35.216929924125424 36.0 36.0 36.0 36.0 36.0 16 35.24092598517546 36.0 36.0 36.0 36.0 36.0 17 35.22065588311498 36.0 36.0 36.0 36.0 36.0 18 35.21533664099403 36.0 36.0 36.0 36.0 36.0 19 35.20668422843328 36.0 36.0 36.0 36.0 36.0 20 35.20327176265245 36.0 36.0 36.0 36.0 36.0 21 35.209192174103066 36.0 36.0 36.0 36.0 36.0 22 35.18473048343419 36.0 36.0 36.0 36.0 36.0 23 35.15803377059489 36.0 36.0 36.0 36.0 36.0 24 35.14288836045818 36.0 36.0 36.0 36.0 36.0 25 35.12454255347595 36.0 36.0 36.0 36.0 36.0 26 35.084366554700416 36.0 36.0 36.0 36.0 36.0 27 35.07481607630056 36.0 36.0 36.0 36.0 36.0 28 35.06025984898848 36.0 36.0 36.0 36.0 36.0 29 35.040140039260415 36.0 36.0 36.0 36.0 36.0 30 35.021353497708276 36.0 36.0 36.0 36.0 36.0 31 35.019408917775344 36.0 36.0 36.0 36.0 36.0 32 35.00255220353454 36.0 36.0 36.0 36.0 36.0 33 34.98074229659593 36.0 36.0 36.0 36.0 36.0 34 34.97401694522997 36.0 36.0 36.0 36.0 36.0 35 34.95840406139672 36.0 36.0 36.0 36.0 36.0 36 34.93956865178557 36.0 36.0 36.0 36.0 36.0 37 34.92187103811432 36.0 36.0 36.0 36.0 36.0 38 34.921098369558706 36.0 36.0 36.0 36.0 36.0 39 34.902137562664066 36.0 36.0 36.0 36.0 36.0 40 34.88589861445222 36.0 36.0 36.0 32.0 36.0 41 34.86716923930873 36.0 36.0 36.0 32.0 36.0 42 34.8419809820267 36.0 36.0 36.0 32.0 36.0 43 34.83425982870362 36.0 36.0 36.0 32.0 36.0 44 34.79739118328012 36.0 36.0 36.0 32.0 36.0 45 34.77700121616924 36.0 36.0 36.0 32.0 36.0 46 34.76960738663763 36.0 36.0 36.0 32.0 36.0 47 34.74423195546921 36.0 36.0 36.0 32.0 36.0 48 34.7224091399867 36.0 36.0 36.0 32.0 36.0 49 34.70519651875012 36.0 36.0 36.0 32.0 36.0 50 34.28193642910951 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 8.0 21 34.0 22 77.0 23 199.0 24 472.0 25 1114.0 26 2401.0 27 4433.0 28 7697.0 29 12628.0 30 19188.0 31 28377.0 32 43782.0 33 74461.0 34 171290.0 35 718390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10857296227395 17.34865261466633 12.149480128867905 26.393294294191815 2 16.556338411291566 19.142159778181654 36.94848551071424 27.353016299812538 3 19.185468627541375 22.2318934120142 27.8902770734406 30.69236088700383 4 13.371499594763925 15.318292420932863 33.581669130047125 37.72853885425608 5 15.343279673745775 35.76948291139299 32.82919322522736 16.058044189633886 6 36.578304365866025 33.30911437923562 15.750680005532248 14.361901249366097 7 31.750868791105646 29.402343638346856 19.23603549111938 19.610752079428114 8 28.658995918134018 33.22253499828962 18.154576124910445 19.963892958665923 9 26.93386123130912 14.775672558187566 18.17449216405284 40.115974046450475 10 17.168932425554516 26.683460083057337 30.116398291644845 26.031209199743305 11 37.34229678033254 21.328141639919856 21.158947511094432 20.17061406865317 12 24.891913995904304 23.19231978520183 27.81145780796328 24.104308410930585 13 30.015591676939717 19.215013005357967 24.506593960829946 26.262801356872373 14 23.76453709500596 18.847857135612553 24.494515251905625 32.89309051747586 15 25.529319452345806 26.520972234644134 21.69114833484394 26.25855997816612 16 26.160948748967307 25.610855806680043 22.895152248318187 25.33304319603446 17 24.702435012396812 25.7672054754355 24.51360145608375 25.016758056083937 18 25.278202684797673 24.938154130142333 25.19236070963929 24.5912824754207 19 26.14100002489505 24.673944011957 24.6824267693695 24.50262919377845 20 26.199413397802406 24.20181254731221 24.693075752085424 24.905698302799962 21 26.538675380548483 23.962498835925953 24.520701155222476 24.97812462830309 22 26.382943018921157 23.800219998469416 24.57583907840373 25.2409979042057 23 25.329513633727448 24.078306915383575 24.911276811737185 25.6809026391518 24 25.290584562274443 24.32344463725568 24.900925555397784 25.485045245072097 25 25.541790527332946 24.011665646276064 24.856991642632163 25.589552183758823 26 25.248721584884365 24.69033125381496 24.966207058068537 25.094740103232137 27 25.520771863566623 24.425104006845274 24.67304231086982 25.381081818718283 28 25.45876846645656 24.149542568513297 24.980867358699683 25.410821606330458 29 25.21656467342647 24.597044485772425 24.96429391387572 25.222096926925385 30 25.3564644918583 24.550685083076694 24.92771128773489 25.16513913733011 31 25.469804443698614 24.2434859900032 24.690400692639418 25.596308873658774 32 25.58079709075888 24.405095597781187 24.396428360675085 25.617678950784846 33 25.28433477878823 24.316374824927227 24.67385060518243 25.725439791102104 34 25.767740602980417 24.212988660693046 24.697154385337274 25.32211635098926 35 25.70335817627083 24.30895111046363 24.9068044077389 25.08088630552664 36 25.259208649477987 24.55587611831993 24.76434948656172 25.42056574564036 37 25.803566489018387 24.304497759418737 24.574473970531287 25.317461781031593 38 25.301944672136816 24.325505530850037 24.725393779741516 25.647156017271634 39 25.72466006419294 24.296869741448702 24.506727718495256 25.471742475863106 40 25.80971146638292 24.303099986814843 24.677171147929148 25.210017398873084 41 25.278297968076757 24.635444184118782 24.806207983299817 25.280049864504644 42 25.824813183732786 24.506034183872945 24.59861243277934 25.070540199614932 43 25.609447988300666 24.153116718579703 24.681652291458043 25.555783001661585 44 25.326807480265227 24.51418017587715 24.57476069771795 25.584251646139673 45 25.290377903894296 24.548769749247374 24.65222422512874 25.508628121729586 46 25.41515526484186 24.42330069873328 24.609186987222163 25.5523570492027 47 25.466512007878013 24.48000899922363 24.93467264401985 25.118806348878508 48 25.682753253555074 24.778101734799073 24.283314246302005 25.255830765343852 49 25.349397101550835 24.699611161159268 24.333639769444478 25.617351967845416 50 25.31810956246565 25.02803052376509 24.459766977830068 25.19409293593919 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 37.0 1 39.5 2 42.0 3 100.5 4 159.0 5 185.5 6 212.0 7 158.5 8 105.0 9 149.5 10 194.0 11 332.0 12 470.0 13 919.0 14 1368.0 15 1693.0 16 2018.0 17 1909.0 18 1800.0 19 1642.0 20 1484.0 21 1752.5 22 2021.0 23 2602.5 24 3184.0 25 4090.5 26 4997.0 27 6044.0 28 7091.0 29 8506.5 30 9922.0 31 11638.5 32 13355.0 33 15567.0 34 17779.0 35 20752.5 36 23726.0 37 27765.0 38 31804.0 39 36167.5 40 40531.0 41 45123.5 42 49716.0 43 53365.0 44 57014.0 45 62626.0 46 68238.0 47 72875.5 48 77513.0 49 81725.0 50 85937.0 51 87830.0 52 89723.0 53 90670.0 54 91617.0 55 89819.5 56 88022.0 57 84650.5 58 81279.0 59 75252.0 60 69225.0 61 61186.5 62 53148.0 63 45672.5 64 38197.0 65 32226.0 66 26255.0 67 21919.0 68 17583.0 69 14595.0 70 11607.0 71 9002.0 72 6397.0 73 5375.5 74 4354.0 75 3444.5 76 2535.0 77 2093.5 78 1652.0 79 1362.0 80 1072.0 81 805.0 82 538.0 83 429.0 84 320.0 85 247.5 86 175.0 87 124.0 88 73.0 89 48.5 90 24.0 91 17.0 92 10.0 93 9.0 94 8.0 95 8.5 96 9.0 97 8.0 98 7.0 99 6.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03153372864212261 2 0.0057166408649462035 3 2.766116547554615E-4 4 0.0 5 0.0 6 2.766116547554615E-4 7 0.0 8 0.0 9 0.0 10 9.220388491848716E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 8.298349642663844E-4 17 0.0 18 1.8440776983697432E-4 19 0.0 20 1.8440776983697432E-4 21 0.0 22 0.0 23 0.0 24 0.0013830582737773074 25 9.220388491848716E-5 26 6.454271944294101E-4 27 8.298349642663844E-4 28 0.001751873813451256 29 3.6881553967394864E-4 30 0.0011986505039403331 31 5.53223309510923E-4 32 8.298349642663844E-4 33 3.6881553967394864E-4 34 6.454271944294101E-4 35 5.53223309510923E-4 36 1.8440776983697432E-4 37 0.0011986505039403331 38 0.0 39 9.220388491848715E-4 40 0.0 41 0.0012908543888588203 42 0.005993252519701665 43 0.004333582591168897 44 0.004241378706250409 45 0.0016596699285327688 46 0.001751873813451256 47 0.0013830582737773074 48 0.004333582591168897 49 0.0038725631665764604 50 0.0019362815832882302 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1084553.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.565445519612624 #Duplication Level Percentage of deduplicated Percentage of total 1 78.01916544693758 48.03284679809079 2 12.851113860124489 15.823691004436657 3 3.9287603749750737 7.256276484754223 4 1.7186957217316352 4.232490712842411 5 0.9820164197088828 3.022913919347613 6 0.5662849335782056 2.0918150536071884 7 0.38674599863734566 1.6667132796324975 8 0.2629479000826581 1.2950803693628345 9 0.2194793636793107 1.2161110326550062 >10 0.9945427851654479 10.43582358899013 >50 0.045990429643835394 1.9476443580453842 >100 0.02335278770005059 2.6455980987565746 >500 9.039780355374326E-4 0.3329952994785408 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8440776983697432E-4 2 0.0 0.0 0.0 0.0 1.8440776983697432E-4 3 0.0 0.0 0.0 0.0 1.8440776983697432E-4 4 0.0 0.0 0.0 0.0 1.8440776983697432E-4 5 0.0 0.0 0.0 0.0 1.8440776983697432E-4 6 0.0 0.0 0.0 0.0 1.8440776983697432E-4 7 0.0 0.0 0.0 0.0 1.8440776983697432E-4 8 0.0 0.0 0.0 0.0 1.8440776983697432E-4 9 0.0 0.0 0.0 0.0 1.8440776983697432E-4 10 0.0 0.0 0.0 0.0 1.8440776983697432E-4 11 0.0 0.0 0.0 0.0 2.7661165475546145E-4 12 0.0 0.0 0.0 0.0 5.532233095109229E-4 13 0.0 0.0 0.0 9.220388491848716E-5 5.532233095109229E-4 14 0.0 0.0 0.0 9.220388491848716E-5 5.532233095109229E-4 15 0.0 0.0 0.0 9.220388491848716E-5 5.532233095109229E-4 16 0.0 0.0 0.0 9.220388491848716E-5 7.376310793478973E-4 17 0.0 0.0 0.0 9.220388491848716E-5 7.376310793478973E-4 18 0.0 0.0 0.0 9.220388491848716E-5 8.298349642663844E-4 19 0.0 0.0 0.0 1.8440776983697432E-4 8.298349642663844E-4 20 0.0 0.0 0.0 4.6101942459243577E-4 8.298349642663844E-4 21 0.0 0.0 0.0 4.6101942459243577E-4 8.298349642663844E-4 22 0.0 0.0 0.0 8.298349642663844E-4 8.298349642663844E-4 23 0.0 0.0 0.0 9.220388491848715E-4 8.298349642663844E-4 24 0.0 0.0 0.0 0.001198650503940333 8.298349642663844E-4 25 0.0 0.0 0.0 0.001751873813451256 8.298349642663844E-4 26 0.0 0.0 0.0 0.001844077698369743 9.220388491848715E-4 27 0.0 0.0 0.0 0.0022128932380436916 9.220388491848715E-4 28 0.0 0.0 0.0 0.0025817087777176406 9.220388491848715E-4 29 0.0 0.0 0.0 0.0032271359721470503 9.220388491848715E-4 30 0.0 0.0 0.0 0.0057166408649462035 9.220388491848715E-4 31 0.0 0.0 0.0 0.010511242880707535 0.0010142427341033587 32 0.0 0.0 0.0 0.015121437126631894 0.0011064466190218458 33 0.0 0.0 0.0 0.020561466336822636 0.001198650503940333 34 0.0 0.0 0.0 0.02664692274144279 0.0013830582737773074 35 0.0 0.0 0.0 0.03347001022541084 0.0013830582737773074 36 0.0 0.0 0.0 0.04352023368152594 0.0013830582737773074 37 0.0 0.0 0.0 0.059194894117668755 0.0013830582737773074 38 0.0 0.0 0.0 0.08123162261318718 0.0015674660436142817 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 590 0.0 27.984016 1 GTATCAA 1300 0.0 25.400877 1 TCTAGCG 150 0.0 21.999884 28 CTAGCGG 155 1.8189894E-12 21.291191 29 CGCAATA 160 1.8189894E-12 20.625841 36 TAGCGGC 165 3.6379788E-12 20.000818 30 TCGATCG 55 0.004482259 20.000818 41 ATACGAA 185 2.1827873E-11 17.838566 40 TATCAAC 1975 0.0 17.15434 2 TCAACGC 1955 0.0 16.766403 4 TACGAAT 210 9.094947E-12 16.76259 41 ATCAACG 1965 0.0 16.681078 3 AATACGA 200 7.2759576E-11 16.500675 39 ACACCGT 200 7.2759576E-11 16.499153 6 TATACTA 120 6.4084415E-6 16.499153 5 CAACGCA 2010 0.0 16.307621 5 GCGCAAT 205 1.05501385E-10 16.098219 35 AACGCAG 2065 0.0 15.873277 6 GGCGCAA 310 0.0 15.612821 34 ACCTAAG 85 0.0029669846 15.539361 1 >>END_MODULE