FastQCFastQC Report
Thu 2 Feb 2017
SRR4062195_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062195_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655545
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCG38560.5882128610545424No Hit
GTGTATATCAATGAGTTACAATGAG29970.45717685284763054No Hit
TTGTAGAACAGTGTATATCAATGAG28830.4397867423289019No Hit
GTATCAACGCAGAGTACTTTTTTTT26930.41080322479768744No Hit
GTCCTACAGTGTGCATTTCTCATTT20480.3124118100206698No Hit
GTCCTAAAGTGTGTATTTCTCATTT18740.2858690097552418No Hit
TATCAACGCAGAGTACTTTTTTTTT18250.2783943131287707No Hit
CTGTAGGACGTGGAATATGGCAAGA17970.27412305791364433No Hit
GTGTATATCAATGAGTTACAATGAA17470.26649581645806164No Hit
GTTCTACAAATCCCGTTTCCAACGA16930.2582583956860322No Hit
CCATAGGGTCTTCTCGTCTTATTAT16830.2567329473949157No Hit
GTCCTACAGTGGACATTTCTAAATT16000.24407172657864828No Hit
GTTCTACAGTGTGGTTTTTATCATT15880.24224118862930843No Hit
CTTTAGGACGTGAAATATGGCGAGG14920.22759688503458955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11910.18168089147198133No Hit
CTGTAGGACCTGGAATATGGCGAGA11090.1691722154848256No Hit
GGTATCAACGCAGAGTACTTTTTTT11040.16840949133926733No Hit
TTGATATACACTGTTCTACAAATCC10870.16581622924436917No Hit
GATATACACTGTTCTACAATGCCGG10820.16505350509881092No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10310.1572737188141165No Hit
GTATTAGAGGCACTGCCTGCCCAGT10090.15391773257366007No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9270.14140905658650435No Hit
GATTAGATGAGTGAGTTACACTGAA8770.1337818151309216No Hit
CTGTAGAACAGATTAGATGAGTGAG8700.13271400132714No Hit
GTAGAACAGTGTATATCAATGAGTT8650.13195127718158173No Hit
GTTATATAATTTAAGCTCCATAGGG8580.13088346337780016No Hit
ATTTAGAAATGTCCACTGTAGGACG8340.1272223874791204No Hit
GTATATCAATGAGTTACAATGAGAA8210.1252393047006689No Hit
GTACATGGGGTGGTATCAACGCAAA8170.12462912538422229No Hit
GTCAGGATACCGCGGCCGTTAAACT8150.12432403572599898No Hit
CCATTGGGATGTCCTGATCCAACAT7940.12112059431465422No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7900.12051041499820761No Hit
CTGAAGGACCTGGAATATGGCGAGA7760.11837478739064444No Hit
CTGTAGAACATATTAGATGAGTGAG7710.11761206324508615No Hit
GTATCAACGCAGAGTACATGGGGTG7520.1147137114919647No Hit
GTCCTTCAGTGTGCATTTCTCATTT7270.11090009076417331No Hit
CTCTAATACTTGTAATGCTAGAGGT7190.10967973213128009No Hit
GTATCAACGCAGAGTACATGGGAGA6790.10357793896681389No Hit
CTTCTACACCATTGGGATGTCCTGA6780.10342539413770221No Hit
ATCTATAACTTTATAGATGCAACAC6700.10220503550480897No Hit
CACTATAAATAATCCACCTATAACT6580.10037449755546911No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGCA250.006024508719.0021416
GACCGGG250.006024508719.002147
AAAGCGT307.7143236E-419.00068710
GCGTTCA350.002166764316.28754813
TAGGGAG350.002167870816.2863035
AGTGGCC601.4630932E-615.8339068
TAGCCTA508.698974E-515.200555
CTAGCCT508.7046465E-515.19938854
CCAACGA3200.015.1388619
AGCACTT456.7452574E-414.78057112
TAACTTT1101.2732926E-1114.6834716
TTGCTAA851.7096681E-814.52993810
TAGAGTG1450.014.4143145
TTCGATT400.0052681714.25269412
GCCGAGT400.00527084214.25160513
AGCTGCT400.00527084214.2516057
AGCCTAT602.56106E-514.2516056
ACCGGGC400.005273515414.2505168
CTAGAAC400.005278864414.2483393
ACTATAC602.5666028E-514.2483393
Produced by FastQC (version 0.11.5)