##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062195_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	655545
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.213323265374612	32.0	32.0	32.0	32.0	32.0
2	30.936041004050065	32.0	32.0	32.0	32.0	32.0
3	30.96576588945076	32.0	32.0	32.0	32.0	32.0
4	30.94357519315989	32.0	32.0	32.0	32.0	32.0
5	30.98021493566422	32.0	32.0	32.0	32.0	32.0
6	34.612330198537094	36.0	36.0	36.0	32.0	36.0
7	34.49107078842795	36.0	36.0	36.0	32.0	36.0
8	34.43712330961261	36.0	36.0	36.0	32.0	36.0
9	34.47698632435607	36.0	36.0	36.0	32.0	36.0
10	34.30058500941964	36.0	36.0	36.0	32.0	36.0
11	34.52995904171338	36.0	36.0	36.0	32.0	36.0
12	34.381429192503944	36.0	36.0	36.0	32.0	36.0
13	34.44513954038243	36.0	36.0	36.0	32.0	36.0
14	34.42148441373209	36.0	36.0	36.0	32.0	36.0
15	34.38275023072406	36.0	36.0	36.0	32.0	36.0
16	34.39648536713727	36.0	36.0	36.0	32.0	36.0
17	34.325576428773005	36.0	36.0	36.0	32.0	36.0
18	34.368871702171475	36.0	36.0	36.0	32.0	36.0
19	34.27366847432289	36.0	36.0	36.0	32.0	36.0
20	34.229187927602226	36.0	36.0	36.0	32.0	36.0
21	34.157928136131005	36.0	36.0	36.0	32.0	36.0
22	34.13562608211488	36.0	36.0	36.0	32.0	36.0
23	34.131452455590384	36.0	36.0	36.0	32.0	36.0
24	34.11359708334287	36.0	36.0	36.0	32.0	36.0
25	33.777318109359385	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	9.0
5	60.0
6	130.0
7	32.0
8	120.0
9	92.0
10	55.0
11	16.0
12	27.0
13	31.0
14	192.0
15	228.0
16	295.0
17	424.0
18	589.0
19	808.0
20	1103.0
21	1472.0
22	2068.0
23	2832.0
24	3673.0
25	4948.0
26	6684.0
27	8109.0
28	11034.0
29	14375.0
30	18905.0
31	26148.0
32	37757.0
33	55633.0
34	134412.0
35	323284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.562566262468785	21.24502507104862	12.886381699278607	24.306026967203987
2	12.233046141830297	22.392242168336526	45.14372795723857	20.23098373259461
3	19.183362774620086	27.26278407790805	31.102660088903534	22.45119305856833
4	9.946755763040262	18.948083054907165	41.72654736296093	29.378613819091644
5	10.104925197306981	41.28309050179758	37.007495528929205	11.604488771966233
6	26.876438658940906	42.7633547236859	19.037006734602915	11.323199882770286
7	24.197304623372656	33.89012331538961	22.37009888138052	19.542473179857215
8	22.089802051867846	41.36798316478777	20.751567611153025	15.790647172191358
9	25.656941162814494	15.506178797963768	21.200661637690743	37.63621840153099
10	13.965435070846802	30.573037277820887	34.83362152716252	20.627906124169787
11	33.13020922445188	23.3272431745411	26.85469777332388	16.68784982768313
12	21.823883807184448	26.730274534244984	34.176397265045	17.269444393525564
13	28.410002153661452	22.875550443791727	27.9401679244966	20.774279478050218
14	19.2677056160693	25.42855135590839	28.328985710882087	26.97475731714022
15	22.246543954785	34.087374933170395	24.481325899335523	19.18475521270908
16	19.85264694831952	29.052132556654865	31.343680221884863	19.75154027314075
17	17.84154157273166	29.812832591017674	30.427152257189466	21.9184735790612
18	17.633574379376437	30.59202639034187	33.694724222454695	18.079675007827
19	22.863438410466586	26.904612114511885	27.76552794082616	22.46642153419537
20	21.105213814332874	31.473445368399517	29.400348748079903	18.02099206918771
21	22.420364076113344	27.23195881011058	27.611548315972385	22.736128797803694
22	20.700255174109373	30.74962548503696	28.809935755985748	19.740183584867914
23	22.31172412108406	29.550995980048338	28.622116909904534	19.515162988963073
24	22.13345792908425	29.774414506854292	28.914612210994356	19.1775153530671
25	21.648222116359463	29.70565062845835	30.36037108969426	18.285756165487925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1340.0
1	1340.0
2	928.5
3	517.0
4	517.0
5	517.0
6	1424.5
7	2332.0
8	2332.0
9	2332.0
10	2379.0
11	2426.0
12	2426.0
13	2426.0
14	3009.0
15	3592.0
16	3592.0
17	3592.0
18	6811.5
19	10031.0
20	10031.0
21	10031.0
22	16022.5
23	22014.0
24	22014.0
25	22014.0
26	36819.5
27	51625.0
28	51625.0
29	51625.0
30	67746.5
31	83868.0
32	83868.0
33	83868.0
34	83196.0
35	82524.0
36	82524.0
37	82524.0
38	81645.5
39	80767.0
40	80767.0
41	80767.0
42	76786.5
43	72806.0
44	72806.0
45	72806.0
46	74527.5
47	76249.0
48	76249.0
49	76249.0
50	69154.0
51	62059.0
52	62059.0
53	62059.0
54	51447.5
55	40836.0
56	40836.0
57	40836.0
58	33855.5
59	26875.0
60	26875.0
61	26875.0
62	22127.0
63	17379.0
64	17379.0
65	17379.0
66	13503.5
67	9628.0
68	9628.0
69	9628.0
70	7257.5
71	4887.0
72	4887.0
73	4887.0
74	3575.5
75	2264.0
76	2264.0
77	2264.0
78	1530.5
79	797.0
80	797.0
81	797.0
82	538.5
83	280.0
84	280.0
85	280.0
86	208.0
87	136.0
88	136.0
89	136.0
90	107.5
91	79.0
92	79.0
93	79.0
94	50.5
95	22.0
96	22.0
97	22.0
98	117.0
99	212.0
100	212.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012203586328932415
2	1.5254482911165518E-4
3	4.576344873349655E-4
4	0.01144086218337414
5	0.033712407233675794
6	0.06422137305600684
7	0.10983227696039173
8	0.11090009076417331
9	0.12295113226399408
10	0.1362225323967081
11	0.1429345048776209
12	0.13866324966249458
13	0.12905292542846028
14	0.1191375115362027
15	0.1365276220549314
16	0.12066295982731925
17	0.1276800219664554
18	0.11593407012485794
19	0.10083213204280408
20	0.09625578716945442
21	0.0956456078530078
22	0.10647629071993533
23	0.08573019396075021
24	0.0956456078530078
25	0.09289980092899801
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	655545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.796964861340747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.321478952891695	14.354681150260692
2	14.166991399758214	6.742627930619255
3	7.179234850446299	5.1253199840215045
4	4.3485615684118955	4.139302673634987
5	2.7463564299854806	3.267747373054085
6	1.993501747701038	2.8463574624637777
7	1.4456113088055236	2.4080813063161295
8	1.0973666012489003	2.08911955599432
9	0.8387359646964487	1.7963433251841712
>10	4.966913655982245	22.35820709699634
>50	0.5022120234552039	8.266294515378846
>100	0.3487226710181275	16.037885942398113
>500	0.030826313460165965	4.853958847509614
>1k	0.013486512138822609	5.714072836168162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATATACACTGTTCTACAAATCCCG	3856	0.5882128610545424	No Hit
GTGTATATCAATGAGTTACAATGAG	2997	0.45717685284763054	No Hit
TTGTAGAACAGTGTATATCAATGAG	2883	0.4397867423289019	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2693	0.41080322479768744	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2048	0.3124118100206698	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1874	0.2858690097552418	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1825	0.2783943131287707	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1797	0.27412305791364433	No Hit
GTGTATATCAATGAGTTACAATGAA	1747	0.26649581645806164	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1693	0.2582583956860322	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1683	0.2567329473949157	No Hit
GTCCTACAGTGGACATTTCTAAATT	1600	0.24407172657864828	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1588	0.24224118862930843	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1492	0.22759688503458955	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1191	0.18168089147198133	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1109	0.1691722154848256	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1104	0.16840949133926733	No Hit
TTGATATACACTGTTCTACAAATCC	1087	0.16581622924436917	No Hit
GATATACACTGTTCTACAATGCCGG	1082	0.16505350509881092	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1031	0.1572737188141165	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1009	0.15391773257366007	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	927	0.14140905658650435	No Hit
GATTAGATGAGTGAGTTACACTGAA	877	0.1337818151309216	No Hit
CTGTAGAACAGATTAGATGAGTGAG	870	0.13271400132714	No Hit
GTAGAACAGTGTATATCAATGAGTT	865	0.13195127718158173	No Hit
GTTATATAATTTAAGCTCCATAGGG	858	0.13088346337780016	No Hit
ATTTAGAAATGTCCACTGTAGGACG	834	0.1272223874791204	No Hit
GTATATCAATGAGTTACAATGAGAA	821	0.1252393047006689	No Hit
GTACATGGGGTGGTATCAACGCAAA	817	0.12462912538422229	No Hit
GTCAGGATACCGCGGCCGTTAAACT	815	0.12432403572599898	No Hit
CCATTGGGATGTCCTGATCCAACAT	794	0.12112059431465422	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	790	0.12051041499820761	No Hit
CTGAAGGACCTGGAATATGGCGAGA	776	0.11837478739064444	No Hit
CTGTAGAACATATTAGATGAGTGAG	771	0.11761206324508615	No Hit
GTATCAACGCAGAGTACATGGGGTG	752	0.1147137114919647	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	727	0.11090009076417331	No Hit
CTCTAATACTTGTAATGCTAGAGGT	719	0.10967973213128009	No Hit
GTATCAACGCAGAGTACATGGGAGA	679	0.10357793896681389	No Hit
CTTCTACACCATTGGGATGTCCTGA	678	0.10342539413770221	No Hit
ATCTATAACTTTATAGATGCAACAC	670	0.10220503550480897	No Hit
CACTATAAATAATCCACCTATAACT	658	0.10037449755546911	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.5254482911165518E-4	0.0
8	0.0	0.0	0.0	4.5763448733496557E-4	0.0
9	0.0	0.0	0.0	4.5763448733496557E-4	0.0
10	0.0	0.0	0.0	4.5763448733496557E-4	0.0
11	0.0	0.0	0.0	4.5763448733496557E-4	0.0
12	0.0	0.0	0.0	4.5763448733496557E-4	0.0
13	0.0	0.0	0.0	6.101793164466207E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGAGCA	25	0.0060245087	19.00214	16
GACCGGG	25	0.0060245087	19.00214	7
AAAGCGT	30	7.7143236E-4	19.000687	10
GCGTTCA	35	0.0021667643	16.287548	13
TAGGGAG	35	0.0021678708	16.286303	5
AGTGGCC	60	1.4630932E-6	15.833906	8
TAGCCTA	50	8.698974E-5	15.20055	5
CTAGCCT	50	8.7046465E-5	15.1993885	4
CCAACGA	320	0.0	15.13886	19
AGCACTT	45	6.7452574E-4	14.780571	12
TAACTTT	110	1.2732926E-11	14.683471	6
TTGCTAA	85	1.7096681E-8	14.529938	10
TAGAGTG	145	0.0	14.414314	5
TTCGATT	40	0.00526817	14.252694	12
GCCGAGT	40	0.005270842	14.251605	13
AGCTGCT	40	0.005270842	14.251605	7
AGCCTAT	60	2.56106E-5	14.251605	6
ACCGGGC	40	0.0052735154	14.250516	8
CTAGAAC	40	0.0052788644	14.248339	3
ACTATAC	60	2.5666028E-5	14.248339	3
>>END_MODULE