FastQCFastQC Report
Thu 2 Feb 2017
SRR4062195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655545
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG19980.30478456856508707No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC19480.2971573271095043No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG16390.2500209749140028No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA13210.20151171925649652No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.1854945121997727No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT10430.15910425676345638No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA8540.13027328406135355No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC8440.128747835770237No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC8300.12661220816267382No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA7690.11730697358686283No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC7680.11715442875775117No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA7670.11700188392863953No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC7620.11623915978308125No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC7230.1102899114477267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.10769664935282856No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA6860.10464575277059547No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6730.10266266999214395No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6690.10205249067569731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACA250.002351809835.2006126
GGTGAGC250.0023526935.1979268
CTCTAGG1400.031.4387111
AGGGGTC1250.029.9182365
CACGTTA407.027963E-427.50047728
TAAGACC757.0062015E-826.3984454
CTAGGAC2300.025.8245663
ATAGGGG1550.025.546883
GTAAGAC701.1315278E-625.1413753
CGAGTGT450.001397390224.4448715
TAGGACC5850.024.4430054
GTGTTAC551.5890523E-424.0077441
TTTAGAG752.062925E-623.4652842
GCGTTTA1600.023.37362335
TAGGGCG1600.023.37362331
TAGGACA5300.023.2439144
TCCTACA13050.022.9258522
CCTACAG13100.022.8383483
GGGCGTT1550.022.7083433
GTATAGG1650.022.6739771
Produced by FastQC (version 0.11.5)