FastQCFastQC Report
Thu 2 Feb 2017
SRR4062194_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062194_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878083
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17800.20271432199461784No Hit
TATCAACGCAGAGTACTTTTTTTTT11220.1277783535269445No Hit
GCGCAAGACGGACCAGAGCGAAAGC9190.10465981006351337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT350.002174714816.2795091
CGAACGA651.9125764E-716.07537816
TAACGAA601.4647576E-615.83361813
GCTATAC508.722655E-515.1968093
CCGATAA653.3614742E-614.6181489
CCAACGA653.3694414E-614.61481419
ACGAACG653.3694414E-614.61481415
GTATTAG1058.913048E-1114.4706751
TTCTCGG400.005280039714.248631516
TTATACT707.25684E-613.5708984
TCCAACG707.25684E-613.57089818
CCAGTAC707.269675E-613.5685793
CCGATCA500.001496504313.3025159
AGGGGCG1153.765308E-1013.217635
TAGACTG655.4462864E-513.1540825
TTTATAC655.457955E-513.1510833
CGTTATT957.403651E-812.9957882
CGATAAC751.4739748E-512.66906310
AACGAAC751.4774363E-512.66617314
ATTATAC751.4800373E-512.6640063
Produced by FastQC (version 0.11.5)