##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062194_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	878083
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229166263325904	32.0	32.0	32.0	32.0	32.0
2	30.728436833420076	32.0	32.0	32.0	32.0	32.0
3	30.7691835509855	32.0	32.0	32.0	32.0	32.0
4	30.765335395401117	32.0	32.0	32.0	32.0	32.0
5	30.677783307500544	32.0	32.0	32.0	32.0	32.0
6	34.358487751157924	36.0	36.0	36.0	32.0	36.0
7	34.278305126053006	36.0	36.0	36.0	32.0	36.0
8	34.21844859768382	36.0	36.0	36.0	32.0	36.0
9	34.36187923009556	36.0	36.0	36.0	32.0	36.0
10	34.02919427890074	36.0	36.0	36.0	32.0	36.0
11	34.360877046930646	36.0	36.0	36.0	32.0	36.0
12	34.158733286033325	36.0	36.0	36.0	32.0	36.0
13	34.25312185750094	36.0	36.0	36.0	32.0	36.0
14	34.15392622337524	36.0	36.0	36.0	32.0	36.0
15	34.098338084213	36.0	36.0	36.0	32.0	36.0
16	34.108487466446796	36.0	36.0	36.0	32.0	36.0
17	34.040439229548916	36.0	36.0	36.0	32.0	36.0
18	34.047698224427535	36.0	36.0	36.0	32.0	36.0
19	34.048814292043005	36.0	36.0	36.0	32.0	36.0
20	34.02176559619079	36.0	36.0	36.0	32.0	36.0
21	34.00235399159305	36.0	36.0	36.0	32.0	36.0
22	33.97049026117121	36.0	36.0	36.0	32.0	36.0
23	33.9436260581289	36.0	36.0	36.0	32.0	36.0
24	33.90486890191474	36.0	36.0	36.0	32.0	36.0
25	33.54554979426774	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	11.0
5	70.0
6	181.0
7	47.0
8	139.0
9	93.0
10	74.0
11	19.0
12	34.0
13	31.0
14	176.0
15	241.0
16	343.0
17	460.0
18	681.0
19	884.0
20	1329.0
21	1851.0
22	2827.0
23	4006.0
24	5683.0
25	7989.0
26	11100.0
27	13741.0
28	18140.0
29	23894.0
30	30820.0
31	41729.0
32	59059.0
33	83289.0
34	182238.0
35	386903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.97297494288636	17.652954079041177	11.702430546855961	25.671640431216503
2	16.49164484825432	19.504920388893506	38.15786926263067	25.845565500221507
3	19.523255827195896	23.15105326274	28.029532582984938	29.296158327079162
4	12.716885459875762	15.448098970612689	35.58926102337363	36.24575454613792
5	14.817726133515608	36.57917521075416	33.38300296195033	15.220095693779903
6	34.555221417835355	35.02983878962884	16.675650020682607	13.739289771853198
7	30.582022886926545	30.264756914756575	19.981030162497635	19.17219003581925
8	27.915376029896795	33.885841776387416	18.895720349175914	19.30306184453988
9	27.127139224919333	14.546729451469096	18.748560548188856	39.577570775422714
10	16.55814388410451	26.824095022624434	30.86456356736243	25.75319752590863
11	37.103806129722024	21.37887384176764	22.158460441910194	19.35885958660014
12	24.593760619239955	23.547059910188313	28.80656277581516	23.052616694756576
13	29.414716024155734	19.834750323840105	25.125545054824762	25.6249885971794
14	23.19248055492848	20.1675776377687	25.02160563502325	31.61833617227957
15	24.994269984640137	27.889951776646853	22.07270334712718	25.04307489158583
16	25.431070358846192	26.019170844445256	23.989095399293777	24.560663397414775
17	23.684264535858198	26.437280938796263	25.365149514616242	24.513305010729294
18	24.23629201890245	25.412393759227488	26.587355424193536	23.763958797676526
19	25.67654139726602	25.02245638673318	25.283611625340836	24.017390590659968
20	25.558308978082557	24.857744057850752	25.1199144640553	24.4640325000114
21	26.211085679894953	24.480002553253573	24.540642325649255	24.768269441202218
22	25.655099614355592	24.90125200488354	25.039071717548246	24.404576663212623
23	24.243950808648375	24.95104799462041	25.67318980157057	25.131811395160646
24	24.621333017207576	25.16801780031187	25.49231267269129	24.71833650978926
25	24.560631637257664	24.984299113569556	25.64994830960438	24.8051209395684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	681.0
1	681.0
2	527.0
3	373.0
4	373.0
5	373.0
6	768.5
7	1164.0
8	1164.0
9	1164.0
10	1320.5
11	1477.0
12	1477.0
13	1477.0
14	1927.0
15	2377.0
16	2377.0
17	2377.0
18	4083.0
19	5789.0
20	5789.0
21	5789.0
22	9298.5
23	12808.0
24	12808.0
25	12808.0
26	19238.0
27	25668.0
28	25668.0
29	25668.0
30	32900.0
31	40132.0
32	40132.0
33	40132.0
34	47973.0
35	55814.0
36	55814.0
37	55814.0
38	64303.0
39	72792.0
40	72792.0
41	72792.0
42	82756.0
43	92720.0
44	92720.0
45	92720.0
46	102601.0
47	112482.0
48	112482.0
49	112482.0
50	115525.5
51	118569.0
52	118569.0
53	118569.0
54	110508.0
55	102447.0
56	102447.0
57	102447.0
58	94060.5
59	85674.0
60	85674.0
61	85674.0
62	74990.0
63	64306.0
64	64306.0
65	64306.0
66	52632.5
67	40959.0
68	40959.0
69	40959.0
70	30779.5
71	20600.0
72	20600.0
73	20600.0
74	15557.5
75	10515.0
76	10515.0
77	10515.0
78	8403.5
79	6292.0
80	6292.0
81	6292.0
82	4342.5
83	2393.0
84	2393.0
85	2393.0
86	1800.5
87	1208.0
88	1208.0
89	1208.0
90	800.5
91	393.0
92	393.0
93	393.0
94	245.0
95	97.0
96	97.0
97	97.0
98	225.0
99	353.0
100	353.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010249600550289665
2	2.2776890111754808E-4
3	2.2776890111754808E-4
4	0.009680178297495795
5	0.03222929950813306
6	0.060814296598385345
7	0.10260988995345544
8	0.10614030792077743
9	0.11536494841603812
10	0.13153654039538404
11	0.13757241627499905
12	0.12948662028532612
13	0.12607008676856288
14	0.11354279720709774
15	0.1277783535269445
16	0.11638990847106709
17	0.11900925083391889
18	0.1079624591297178
19	0.09418244061210615
20	0.09008260039199027
21	0.0878049113808148
22	0.09680178297495794
23	0.07892192423723042
24	0.09053813819422538
25	0.0847300312157279
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	878083.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.29818708915685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9335902076933	46.01685209546943
2	12.893624745416238	15.033498953313163
3	3.773344636126109	6.599374546462392
4	1.4995463133388165	3.496833260955269
5	0.7614039623442654	2.219423532358566
6	0.4570373967365972	1.5986670997014805
7	0.3038770956552417	1.2400838642233225
8	0.203938075235823	0.9511376051760442
9	0.15976536961518584	0.8382628267374961
>10	0.8654328211685895	9.160534912528123
>50	0.07854124683766009	3.258018331365838
>100	0.06400783802785558	7.222706476425926
>500	0.00549760568400785	2.0324306772313245
>1k	3.926861202862751E-4	0.33217581805166163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1780	0.20271432199461784	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1122	0.1277783535269445	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	919	0.10465981006351337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1388445055877406E-4
2	0.0	0.0	0.0	0.0	1.1388445055877406E-4
3	0.0	0.0	0.0	0.0	1.1388445055877406E-4
4	0.0	0.0	0.0	0.0	1.1388445055877406E-4
5	0.0	0.0	0.0	0.0	2.277689011175481E-4
6	0.0	0.0	0.0	0.0	2.277689011175481E-4
7	0.0	0.0	0.0	0.0	2.277689011175481E-4
8	0.0	0.0	0.0	0.0	2.277689011175481E-4
9	0.0	0.0	0.0	0.0	2.277689011175481E-4
10	0.0	0.0	0.0	0.0	3.4165335167632215E-4
11	0.0	0.0	0.0	0.0	3.4165335167632215E-4
12	0.0	0.0	0.0	0.0	6.833067033526443E-4
13	0.0	0.0	0.0	1.1388445055877406E-4	6.833067033526443E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAT	35	0.0021747148	16.279509	1
CGAACGA	65	1.9125764E-7	16.075378	16
TAACGAA	60	1.4647576E-6	15.833618	13
GCTATAC	50	8.722655E-5	15.196809	3
CCGATAA	65	3.3614742E-6	14.618148	9
CCAACGA	65	3.3694414E-6	14.614814	19
ACGAACG	65	3.3694414E-6	14.614814	15
GTATTAG	105	8.913048E-11	14.470675	1
TTCTCGG	40	0.0052800397	14.2486315	16
TTATACT	70	7.25684E-6	13.570898	4
TCCAACG	70	7.25684E-6	13.570898	18
CCAGTAC	70	7.269675E-6	13.568579	3
CCGATCA	50	0.0014965043	13.302515	9
AGGGGCG	115	3.765308E-10	13.21763	5
TAGACTG	65	5.4462864E-5	13.154082	5
TTTATAC	65	5.457955E-5	13.151083	3
CGTTATT	95	7.403651E-8	12.995788	2
CGATAAC	75	1.4739748E-5	12.669063	10
AACGAAC	75	1.4774363E-5	12.666173	14
ATTATAC	75	1.4800373E-5	12.664006	3
>>END_MODULE