FastQCFastQC Report
Thu 2 Feb 2017
SRR4062194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878083
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8880.10112939209619136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.026.0785221
GGTATCA6450.020.8146611
TAGGACG5400.020.7779394
ATACACT2350.020.5959054
TACCGAT755.472521E-520.5323247
CGATCAA654.930427E-420.30784810
ATACGAA2300.020.08711240
GTAGGAC4950.020.0001543
TACCGTC2150.019.4409057
TTAGGAC4000.019.2501493
GATATAC2300.019.1382121
GGACGTG5800.018.9656666
CGCAATA2450.018.8572936
AAACCGT708.120484E-418.8572886
ACCGTCG2100.018.8562158
AACGCAG19150.018.496236
AATGCCG1203.161149E-718.33243418
ATCAACG19250.018.2858563
AGGACGT6050.018.181965
ATACCGT2300.018.1740556
Produced by FastQC (version 0.11.5)