##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062194_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	878083
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28678951761963	32.0	32.0	32.0	32.0	32.0
2	31.47010931768409	32.0	32.0	32.0	32.0	32.0
3	31.588870300415792	32.0	32.0	32.0	32.0	32.0
4	31.66307740839989	32.0	32.0	32.0	32.0	32.0
5	31.64362822193346	32.0	32.0	32.0	32.0	32.0
6	35.28467582221726	36.0	36.0	36.0	36.0	36.0
7	35.29339595459655	36.0	36.0	36.0	36.0	36.0
8	35.24800389029283	36.0	36.0	36.0	36.0	36.0
9	35.3219194540835	36.0	36.0	36.0	36.0	36.0
10	35.21038671742876	36.0	36.0	36.0	36.0	36.0
11	35.326512413974534	36.0	36.0	36.0	36.0	36.0
12	35.2422709470517	36.0	36.0	36.0	36.0	36.0
13	35.27687359850948	36.0	36.0	36.0	36.0	36.0
14	35.24650175438996	36.0	36.0	36.0	36.0	36.0
15	35.222365083938534	36.0	36.0	36.0	36.0	36.0
16	35.24120271090546	36.0	36.0	36.0	36.0	36.0
17	35.217198146416685	36.0	36.0	36.0	36.0	36.0
18	35.21582014456492	36.0	36.0	36.0	36.0	36.0
19	35.20111538430877	36.0	36.0	36.0	36.0	36.0
20	35.19279726403996	36.0	36.0	36.0	36.0	36.0
21	35.18997862388863	36.0	36.0	36.0	36.0	36.0
22	35.17327063614715	36.0	36.0	36.0	36.0	36.0
23	35.13737653502004	36.0	36.0	36.0	36.0	36.0
24	35.11898875163282	36.0	36.0	36.0	36.0	36.0
25	35.10320550562988	36.0	36.0	36.0	36.0	36.0
26	35.0650359931806	36.0	36.0	36.0	36.0	36.0
27	35.046861173715925	36.0	36.0	36.0	36.0	36.0
28	35.01800854816686	36.0	36.0	36.0	36.0	36.0
29	34.999945335463735	36.0	36.0	36.0	36.0	36.0
30	34.96940152582387	36.0	36.0	36.0	36.0	36.0
31	34.98706386526103	36.0	36.0	36.0	36.0	36.0
32	34.950666394862445	36.0	36.0	36.0	36.0	36.0
33	34.927691345806714	36.0	36.0	36.0	36.0	36.0
34	34.92019205473742	36.0	36.0	36.0	36.0	36.0
35	34.90754518650287	36.0	36.0	36.0	36.0	36.0
36	34.8756757618585	36.0	36.0	36.0	32.0	36.0
37	34.84956889041241	36.0	36.0	36.0	32.0	36.0
38	34.83134737832301	36.0	36.0	36.0	32.0	36.0
39	34.80029564403365	36.0	36.0	36.0	32.0	36.0
40	34.795552356667876	36.0	36.0	36.0	32.0	36.0
41	34.7702233160191	36.0	36.0	36.0	32.0	36.0
42	34.73722415762519	36.0	36.0	36.0	32.0	36.0
43	34.742715665831135	36.0	36.0	36.0	32.0	36.0
44	34.6782434006808	36.0	36.0	36.0	32.0	36.0
45	34.65690714886861	36.0	36.0	36.0	32.0	36.0
46	34.62108707263437	36.0	36.0	36.0	32.0	36.0
47	34.58981554135543	36.0	36.0	36.0	32.0	36.0
48	34.568636450085016	36.0	36.0	36.0	32.0	36.0
49	34.544216207351695	36.0	36.0	36.0	32.0	36.0
50	34.14670594920981	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	10.0
21	31.0
22	71.0
23	172.0
24	458.0
25	1038.0
26	2254.0
27	4273.0
28	7069.0
29	11061.0
30	16544.0
31	24281.0
32	36343.0
33	61156.0
34	139792.0
35	573522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.802434367324246	18.17846254801974	12.18790016450891	25.8312029201471
2	16.01840905829535	20.0220717365605	37.69608881539236	26.26343038975179
3	18.91042066686255	23.49871652078052	28.550067078017772	29.040795734339163
4	12.60484487229567	15.77538797585194	35.75538986633382	35.864377285518565
5	14.413785484971239	36.85209712521482	33.275555955416515	15.45856143439743
6	34.588231810577405	35.35183053005481	16.487354782428458	13.572582876939318
7	30.507480500134953	30.2225416048369	20.074525984445664	19.195451910582484
8	27.879482919040683	33.76002040809354	18.939211896825242	19.421284776040533
9	27.179662970357015	14.363790211175937	18.687413376639796	39.76913344182725
10	16.481300109329446	26.753598760932945	31.112313228862977	25.652787900874635
11	37.41388911982125	21.125793347553707	22.131734699339354	19.328582833285694
12	24.66281661300811	23.576928376930198	28.787711412246907	22.972543597814784
13	29.52112727384541	19.609763541715306	25.29339481575204	25.57571436868724
14	23.289142370368175	19.932512074598872	25.056173505238117	31.72217204979484
15	24.955727419845278	27.85169511310434	22.009308914988672	25.183268552061705
16	25.429147035745075	25.95062631038893	23.996132466440947	24.62409418742505
17	23.772012440737377	26.303549892208366	25.340429093832817	24.58400857322144
18	24.43994474326459	25.24260235080283	26.687454375041998	23.62999853089059
19	25.675961926107128	24.881844747984815	25.437487615051896	24.00470571085616
20	25.66753750805737	24.58039035256549	25.38248310514556	24.369589034231584
21	26.517311005907185	24.177554969177173	24.733311087903992	24.57182293701165
22	25.653383563968323	24.775562219061296	25.30546656751127	24.265587649459107
23	24.253857551051553	24.675685555921252	25.85723673046853	25.213220162558663
24	24.614925570308756	24.98892466150463	25.905359097209015	24.490790670977603
25	24.791220855479164	24.64897885274821	25.802972618699187	24.756827673073442
26	24.098287283945975	25.690626925367766	26.175663009820298	24.035422780865957
27	24.68465287900875	25.168891217201168	25.554505284256564	24.591950619533527
28	24.44745356582782	25.020527952750975	25.791536219736216	24.74048226168499
29	24.31264961586731	25.11348598422471	25.630977516906167	24.942886883001815
30	24.32525695413262	25.193292144748458	25.912592887851265	24.568858013267658
31	24.918401326533633	24.834012468140642	25.327248832108307	24.920337373217414
32	24.741450881646514	25.18275815336538	25.12456253637226	24.951228428615842
33	24.325546276482466	24.799648324015667	25.886791765224395	24.988013634277475
34	24.852403903096786	25.00706092438889	25.940583460126277	24.199951712388053
35	25.217918144319096	24.80759200165816	25.988461214328503	23.986028639694243
36	24.151958473126655	25.488621791586663	25.575629610901945	24.783790124384737
37	24.96082290129193	25.163883270467757	25.358060460285355	24.517233367954955
38	24.568634172396003	25.119151606397118	25.503056089230746	24.809158131976133
39	25.219258036044756	24.707114995871652	25.19420322865359	24.879423739430003
40	25.602678571428573	24.909803206997083	25.551316508746357	23.93620171282799
41	24.537709279296823	25.100077100651085	26.10876316661599	24.253450453436106
42	25.36826592136523	25.667229287248556	25.38216059970343	23.582344191682783
43	24.725071977841758	24.756733117096104	25.804625715222855	24.71356918983928
44	24.25941427359334	25.433372624721684	25.321534528019313	24.98567857366566
45	24.615859436214215	25.65633029027497	25.353391096776544	24.374419176734268
46	24.587499715281417	25.1039792269321	25.27492426485661	25.03359679292987
47	24.885544693373664	25.0473765249319	25.609972758498163	24.457106023196275
48	25.19887796950291	25.537241003085242	24.685695933379723	24.578185094032122
49	24.704089616458234	25.73762631640687	24.844969495007703	24.713314572127196
50	24.74799988611451	25.934914443527035	24.737294650229195	24.57979102012926
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	365.0
1	212.5
2	60.0
3	147.5
4	235.0
5	242.0
6	249.0
7	186.5
8	124.0
9	180.5
10	237.0
11	377.0
12	517.0
13	1003.5
14	1490.0
15	1816.5
16	2143.0
17	2161.0
18	2179.0
19	2068.5
20	1958.0
21	2304.5
22	2651.0
23	3058.5
24	3466.0
25	4375.0
26	5284.0
27	7099.5
28	8915.0
29	10585.5
30	12256.0
31	14106.5
32	15957.0
33	18658.5
34	21360.0
35	24747.5
36	28135.0
37	31529.5
38	34924.0
39	37237.0
40	39550.0
41	42278.5
42	45007.0
43	46154.0
44	47301.0
45	51409.5
46	55518.0
47	59387.5
48	63257.0
49	65707.0
50	68157.0
51	66527.5
52	64898.0
53	64029.5
54	63161.0
55	63624.0
56	64087.0
57	61387.5
58	58688.0
59	53475.0
60	48262.0
61	41947.5
62	35633.0
63	30777.0
64	25921.0
65	21696.5
66	17472.0
67	15007.0
68	12542.0
69	11209.0
70	9876.0
71	7834.5
72	5793.0
73	5031.0
74	4269.0
75	3126.5
76	1984.0
77	1776.5
78	1569.0
79	1341.0
80	1113.0
81	886.0
82	659.0
83	566.5
84	474.0
85	357.0
86	240.0
87	154.5
88	69.0
89	51.5
90	34.0
91	26.0
92	18.0
93	11.5
94	5.0
95	7.0
96	9.0
97	9.0
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03632913972824892
2	0.00421372467067464
3	1.1388445055877404E-4
4	0.0
5	0.0
6	4.5553780223509617E-4
7	0.0
8	0.0
9	0.0
10	3.416533516763222E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.5553780223509617E-4
17	0.0
18	0.0
19	1.1388445055877404E-4
20	5.694222527938702E-4
21	0.0
22	0.0
23	0.0
24	6.833067033526444E-4
25	2.2776890111754808E-4
26	4.5553780223509617E-4
27	3.416533516763222E-4
28	0.0013666134067052888
29	1.1388445055877404E-4
30	9.110756044701923E-4
31	5.694222527938702E-4
32	0.0011388445055877405
33	2.2776890111754808E-4
34	0.0012527289561465147
35	1.1388445055877404E-4
36	1.1388445055877404E-4
37	0.002163804560616707
38	0.0
39	9.110756044701923E-4
40	3.416533516763222E-4
41	0.0011388445055877405
42	0.005580338077379929
43	0.003985955769557092
44	0.003188764615645674
45	0.002163804560616707
46	0.002619342362851803
47	0.0012527289561465147
48	0.0036443024178807694
49	0.003416533516763222
50	9.110756044701923E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	878083.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.570612186969065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.78703166897549	47.72178740583964
2	13.314951166220695	16.1298948675517
3	3.7248820122411894	6.7685515141703405
4	1.4961145733589243	3.624823024407844
5	0.7337983807408194	2.222330857163902
6	0.44764831540982836	1.6268599505303232
7	0.2767335994423225	1.173334647164747
8	0.2048143939849568	0.9924586582697527
9	0.14714910503654016	0.8021620237345065
>10	0.7430193682699345	8.2233107796613
>50	0.06570733675764562	2.7654387403158935
>100	0.054374100409545295	6.408548635306769
>500	0.003775979152119731	1.540498895883278
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	888	0.10112939209619136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.4165335167632215E-4
13	0.0	0.0	0.0	1.1388445055877406E-4	4.555378022350962E-4
14	0.0	0.0	0.0	1.1388445055877406E-4	4.555378022350962E-4
15	0.0	0.0	0.0	4.555378022350962E-4	5.694222527938702E-4
16	0.0	0.0	0.0	4.555378022350962E-4	6.833067033526443E-4
17	0.0	0.0	0.0	4.555378022350962E-4	6.833067033526443E-4
18	0.0	0.0	0.0	9.110756044701924E-4	0.0011388445055877405
19	0.0	0.0	0.0	0.0010249600550289665	0.0011388445055877405
20	0.0	0.0	0.0	0.0011388445055877405	0.0011388445055877405
21	0.0	0.0	0.0	0.0012527289561465147	0.0012527289561465147
22	0.0	0.0	0.0	0.0013666134067052886	0.0012527289561465147
23	0.0	0.0	0.0	0.0015943823078228368	0.0012527289561465147
24	0.0	0.0	0.0	0.001822151208940385	0.0012527289561465147
25	0.0	0.0	0.0	0.0019360356594991589	0.0012527289561465147
26	0.0	0.0	0.0	0.0030748801650868993	0.0012527289561465147
27	0.0	0.0	0.0	0.003758186868439544	0.0012527289561465147
28	0.0	0.0	0.0	0.004555378022350962	0.0012527289561465147
29	0.0	0.0	0.0	0.007174720385202765	0.0012527289561465147
30	0.0	0.0	0.0	0.010819022803083535	0.0012527289561465147
31	0.0	0.0	0.0	0.017765974287168754	0.0012527289561465147
32	0.0	0.0	0.0	0.023232427913989908	0.0012527289561465147
33	0.0	0.0	0.0	0.02892665044192861	0.0012527289561465147
34	0.0	0.0	0.0	0.0359874863765726	0.0013666134067052886
35	0.0	0.0	0.0	0.04487047352015698	0.0013666134067052886
36	0.0	0.0	0.0	0.05614503412547561	0.0013666134067052886
37	0.0	0.0	0.0	0.07083612824755746	0.0013666134067052886
38	0.0	0.0	0.0	0.0944102095132237	0.0013666134067052886
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1325	0.0	26.078522	1
GGTATCA	645	0.0	20.814661	1
TAGGACG	540	0.0	20.777939	4
ATACACT	235	0.0	20.595905	4
TACCGAT	75	5.472521E-5	20.532324	7
CGATCAA	65	4.930427E-4	20.307848	10
ATACGAA	230	0.0	20.087112	40
GTAGGAC	495	0.0	20.000154	3
TACCGTC	215	0.0	19.440905	7
TTAGGAC	400	0.0	19.250149	3
GATATAC	230	0.0	19.138212	1
GGACGTG	580	0.0	18.965666	6
CGCAATA	245	0.0	18.85729	36
AAACCGT	70	8.120484E-4	18.857288	6
ACCGTCG	210	0.0	18.856215	8
AACGCAG	1915	0.0	18.49623	6
AATGCCG	120	3.161149E-7	18.332434	18
ATCAACG	1925	0.0	18.285856	3
AGGACGT	605	0.0	18.18196	5
ATACCGT	230	0.0	18.174055	6
>>END_MODULE